Publication date: February 2018
Source:Molecular Immunology, Volume 94
Author(s): Yanqin Fu, Chongxian Wang, Dongming Zhang, Yaping Xin, Jun Li, Yuanyuan Zhang, Xiaojing Chu
Although TRPC6 expression is shown to be significantly elevated in a rat model diabetic nephropathy (DN), its expression and role in human DN are unclear. We thus explored the role of TRPC6 in the pathophysiology of tubular epithelial cell injury following DN. HK-2 cells were cultured in a high-glucose medium to induce a DN cell model. Ad-TRPC6 and TRP6 siRNA were transfected to overexpress and knock down TRPC6. We found that TRPC6 expression was significantly upregulated in DN tissues and cells. TRPC6 siRNA inhibited cell proliferation and promoted cell apoptosis in HK-2 cells treated with high glucose, whereas Ad-TRPC6 showed the opposite effect. Furthermore, Ad-TRPC6 significantly promoted release of IL-8 and IL-6. Subsequent experiments demonstrated that the signaling pathway of nuclear factor of activated T cells (NFAT) was activated by Ad-TRPC6 and deactivated by TRPC6 siRNA. The NFAT signaling inhibitor, FK-506, eliminated the effect of TRPC6 on HK-2 cells. These results suggest that TRPC6 was upregulated in DN and could promote cell proliferation and inflammation by inhibiting the NFAT signaling pathway in tubular epithelial cells.
http://ift.tt/2m0Sabk
Αρχειοθήκη ιστολογίου
-
►
2023
(256)
- ► Φεβρουαρίου (140)
- ► Ιανουαρίου (116)
-
►
2022
(1695)
- ► Δεκεμβρίου (78)
- ► Σεπτεμβρίου (142)
- ► Φεβρουαρίου (155)
-
►
2021
(5507)
- ► Δεκεμβρίου (139)
- ► Σεπτεμβρίου (333)
- ► Φεβρουαρίου (628)
-
►
2020
(1810)
- ► Δεκεμβρίου (544)
- ► Σεπτεμβρίου (32)
- ► Φεβρουαρίου (28)
-
►
2019
(7684)
- ► Δεκεμβρίου (18)
- ► Σεπτεμβρίου (53)
- ► Φεβρουαρίου (2841)
- ► Ιανουαρίου (2803)
-
▼
2018
(31838)
- ► Δεκεμβρίου (2810)
- ► Σεπτεμβρίου (2870)
- ► Φεβρουαρίου (2420)
-
▼
Ιανουαρίου
(2395)
-
▼
Ιαν 05
(119)
- The effects of transcutaneous low-level laser ther...
- Comments concerning ‘Meta-analysis of the associat...
- Identifying criteria for diagnosis of post-traumat...
- KLICK syndrome: an unusual phenotype
- Striate palmoplantar keratoderma resulting from a ...
- Long-term management of moderate-to-severe atopic ...
- Pneumocephalus and seizures following combined spi...
- Table of Contents
- Editorial Board
- Nasotracheal intubation through pharyngeal flap af...
- Clinical experiences of unilateral anterior sub-co...
- Clinical effects of prophylactic use of phentolami...
- Emergency insertion of the LMA protector airway in...
- Respiratory effect of interscalene brachial plexus...
- Under pressure: Evaluating the role of intraoperat...
- Incidence of lower-extremity muscle weakness after...
- Lack of generalizability of observational studies'...
- Lidocaine post-nasal dripping (LPND): An easy way ...
- Severe type B lactic acidosis and insulin-resistan...
- Use of dexmedetomidine in a cardiac intensive care...
- Bilateral adductor canal block catheters in patien...
- Videolaryngoscope versus Macintosh laryngoscope fo...
- Retroclavicular approach to infraclavicular brachi...
- Anesthetic management for laparoscopic sleeve gast...
- Ketorolac dose-response for acute pain is well kno...
- Takotsubo cardiomyopathy complicating thoracoabdom...
- Detection of human papillomavirus type 52 in a cas...
- Case of successfully switching from nivolumab to v...
- Two cases of Japanese spotted fever infected in Ro...
- Dabrafenib and trametinib combination therapy safe...
- Isotopic response
- Assessment of a visual risk communication aid used...
- Local Anesthetic Systemic Toxicity: A Review of Re...
- The Prevalence of Cosmetic Facial Plastic Procedur...
- Ablative Fractional CO2 Laser for Facial Atrophic ...
- Complications in Orthognathic Surgery
- Protection of Inferior Alveolar Neurovascular Bund...
- Interpositional Arthroplasty by Temporalis Fascia ...
- Patient Perception and Satisfaction Questionnaire ...
- Wedge Osteotomy for Correction of Chin Asymmetry
- Review of “Readiness of US General Surgery Residen...
- Maxillary Sagittal Position in Relation to the For...
- Reconstruction of a Compound Oromandibular Defect ...
- Surgical Treatment of Synovial Chondromatosis in t...
- Three-Dimensional Radiological Assessment of Alveo...
- Mandibular Osteonecrosis Associated With Raloxifene
- Review of “Modern Surgeons: Still Masters of Their...
- Review of “A Lifetime of Surgical Education: Can W...
- Unusual Craniofacial Distraction
- Peripheral Giant Cell Granuloma Associated With De...
- Facing a New Face: An Interpretative Phenomenologi...
- Factors Influencing Perception of Facial Attractiv...
- Is Osteoplasty With Unilateral Osteotomy Effective...
- The efficacy of pre-operative preparation with int...
- Effect of pressure-controlled inverse ratio ventil...
- Primary mucinous eccrine carcinoma of the buccal s...
- Risk factors of sensorineural hearing loss in pati...
- Dermatomyositis: A Diagnostic Dilemma
- Fluorescence microscopy for the evaluation of elas...
- Reply to “Serious infections among a large cohort ...
- Comment on “Association of bullous pemphigoid with...
- The alveolar bone protective effects of natural pr...
- Epigenetic mechanisms in odontogenic tumors: A lit...
- Influence on proliferation and adhesion of human g...
- Epigenetic mechanisms in odontogenic tumors: A lit...
- Influence on proliferation and adhesion of human g...
- The alveolar bone protective effects of natural pr...
- Modification of the measurement of the major varia...
- Quality improving the provision of the division of...
- Re: Styloid Process Fracture Associated With Maxil...
- Varicocele due to renal arteriovenous malformation...
- Treatment modalities and risk factors associated w...
- What you should know about alopecia totalis
- The Use of Omalizumab in Allergen Immunotherapy
- A Decade of Research on the 17q12-21 Asthma Locus:...
- Role of airway glucose in bacterial infections in ...
- Transplantation and Risk for Malignant Melanoma
- Degree of tinnitus improvement with stapes surgery...
- Comparison of anxiety and child-care education cha...
- Treatment of a complex orthopaedic infection due t...
- Axillary artery aneurysm combined with brachial pl...
- Uterocutaneous fistula following B-Lynch suture fo...
- Efficacy and safety of maintenance electroconvulsi...
- Varicella zoster meningitis: an atypical case of z...
- Postsurgical pyoderma gangrenosum after an autolog...
- Embolic stroke in a postpartum patient with Fontan...
- Rash and subcutaneous fat necrosis after DEB-TACE ...
- Radius-Maumenee syndrome: a rare cause of glaucoma
- Vedolizumab-induced acute pancreatitis: the first ...
- Isolated lateral leg compartment syndrome followin...
- Bilateral secondary spontaneous pneumothorax
- Blue toe syndrome as a first sign of systemic scle...
- A Multicenter, Randomized, Case-control Study of U...
- Vaccination Against Human Papillomavirus (HPV) Wit...
- Early Non Invasive Ventilation and Hematological M...
- Indications for Trans-Nasal Flexible Laryngo-Oesop...
- Asthma onset pattern and patient outcomes in a chr...
- Are multiple sinus cultures necessary during sinus...
- Comparative analysis of the Hom family of outer me...
- Levels of malondialdehyde in the gastric juice: It...
-
▼
Ιαν 05
(119)
-
►
2017
(31987)
- ► Δεκεμβρίου (2460)
- ► Σεπτεμβρίου (2605)
- ► Φεβρουαρίου (2785)
- ► Ιανουαρίου (2830)
-
►
2016
(5308)
- ► Δεκεμβρίου (2118)
- ► Σεπτεμβρίου (877)
- ► Φεβρουαρίου (41)
- ► Ιανουαρίου (39)
Αλέξανδρος Γ. Σφακιανάκης
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5
Άγιος Νικόλαος Κρήτη 72100
2841026182
6032607174
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου