miR-29b/HDAC4 Epigenetic Loop in Multiple Myeloma
Epigenetic abnormalities are common in hematologic malignancies, including multiple myeloma, and their effects can be efficiently counteracted by a class of tumor suppressor miRNAs, named epi-miRNAs. Given the oncogenic role of histone deacetylases (HDAC) in multiple myeloma, we investigated whether their activity could be antagonized by miR-29b, a well-established epi-miRNA. We demonstrated here that miR-29b specifically targets HDAC4 and highlighted that both molecules are involved in a functional loop. In fact, silencing of HDAC4 by shRNAs inhibited multiple myeloma cell surviva...περισσότερα »
Reversal of MDR by osimertinib
The overexpression of ATP-binding cassette (ABC) transporters has been proved to be a major trigger for multidrug resistance (MDR) in certain types of cancer. In our study, we investigated whether osimertinib (AZD9291), a third generation irreversible tyrosine kinase inhibitor of both activating EGFR mutations and resistance-associated T790M point mutation, could reverse MDR induced by ABCB1 and ABCG2 in vitro, in vivo and ex vivo. Our results showed that osimertinib significantly increased the sensitivity of ABCB1- and ABCG2-overexpressing cells to their substrate chemotherapeuti...περισσότερα »
Emodin blocks cancer cell-macrophage interaction
Macrophage infiltration correlates with severity in many types of cancer. Tumor cells recruit macrophages and educate them to adopt an M2-like phenotype through the secretion of chemokines and growth factors, such as MCP1 and CSF1. Macrophages in turn promote tumor growth through supporting angiogenesis, suppressing anti-tumor immunity, modulating extracellular matrix remodeling, and promoting tumor cell migration. Thus tumor cells and macrophages interact to create a feedforward loop supporting tumor growth and metastasis. In this study, we tested the ability of emodin, a Chinese ...περισσότερα »
Low MSLN shedding enhances efficacy of anticancer agents
Mesothelin (MSLN) is a differentiation antigen that is highly expressed in many epithelial cancers. MSLN is an important therapeutic target due to its high expression in cancers and limited expression in normal human tissues. Although it has been assumed that shed antigen is a barrier to immunotoxin action, a modeling study predicted that shed MSLN may enhance the action of MSLN targeting recombinant immunotoxins such as SS1P and similar therapeutics by facilitating their redistribution within tumors. We aimed to determine if shed MSLN enhances or reduces the anti-tumor effect of M...περισσότερα »
An Examination of Sources of Variability Across the Consonant-Nucleus-Consonant Test in Cochlear Implant Listeners
The 10 consonant-nucleus-consonant (CNC) word lists are considered the gold standard in the testing of cochlear implant (CI) users. However, variance in scores across lists could degrade the sensitivity and reliability of them to identify deficits in speech perception. This study examined the relationship between variability in performance among lists and the lexical characteristics of the words. Data are from 28 adult CI users. Each subject was tested on all 10 CNC word lists. Data were analyzed in terms of lexical characteristics, lexical frequency, neighborhood density, bi-, and... περισσότερα »
An Examination of Sources of Variability Across the Consonant-Nucleus-Consonant Test in Cochlear Implant Listeners
The 10 consonant-nucleus-consonant (CNC) word lists are considered the gold standard in the testing of cochlear implant (CI) users. However, variance in scores across lists could degrade the sensitivity and reliability of them to identify deficits in speech perception. This study examined the relationship between variability in performance among lists and the lexical characteristics of the words. Data are from 28 adult CI users. Each subject was tested on all 10 CNC word lists. Data were analyzed in terms of lexical characteristics, lexical frequency, neighborhood density, bi-, and... περισσότερα »
An Examination of Sources of Variability Across the Consonant-Nucleus-Consonant Test in Cochlear Implant Listeners
The 10 consonant-nucleus-consonant (CNC) word lists are considered the gold standard in the testing of cochlear implant (CI) users. However, variance in scores across lists could degrade the sensitivity and reliability of them to identify deficits in speech perception. This study examined the relationship between variability in performance among lists and the lexical characteristics of the words. Data are from 28 adult CI users. Each subject was tested on all 10 CNC word lists. Data were analyzed in terms of lexical characteristics, lexical frequency, neighborhood density, bi-, and... περισσότερα »
Cochleural Alternating Acoustic Beam Therapy (CAABT) Versus Masking Intervention for Tinnitus
Cochleural Alternating Acoustic Beam Therapy (CAABT) Versus Masking Intervention for Tinnitus: *Condition*: Tinnitus, Subjective *Interventions*: Device: CAABT; Device: masking therapy *Sponsor*: Beijing Friendship Hospital ArmsAssigned Interventions Active Comparator: masking therapy Masking intervention was a standard monophone tinnitus masking therapy. Device: masking therapy Masking intervention was a standard monophone tinnitus masking therapy. Experimental: CAABT CAABT was an innovative tinnitus intervention which based on neural science and cochlear plastic research an...περισσότερα »
Postoperative Concurrent Chemoradiotherapy With Docetaxel for High-Risk Squamous Cell Carcinoma of Head and Neck
Postoperative Concurrent Chemoradiotherapy With Docetaxel for High-Risk Squamous Cell Carcinoma of Head and Neck: *Conditions*: Head-and-neck Cancer; Squamous Cell Carcinoma *Interventions*: Drug: Docetaxel; Radiation: Intensity-modulated radiotherapy *Sponsor*: Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University ArmsAssigned Interventions Experimental: Docetaxel Group Concurrent Chemoradiotherapy With Docetaxel Drug: Docetaxel Docetaxel: 20mg/m2/w for 6 weeks Radiation: Intensity-modulated radiotherapy a total dose of 60Gy in 30 fractions over 6 ... περισσότερα »
Apatinib in Metastatic Adenoid Cystic Carcinoma of the Head and Neck
A Study of *Condition*: Adenoid Cystic Carcinoma *Intervention*: Drug: Apatinib Mesylate *Sponsor*: Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University ArmsAssigned Interventions Experimental: Apatinib group Apatinib Mesylate administered as a daily oral treatment Drug: Apatinib Mesylate Apatinib Mesylate will be administered orally at 500 mg once daily for 5 consecutive days, and this was followed by a 2-day rest every week; each cycle consisted of 28 days
Postoperative Concurrent Chemoradiotherapy in Treating Patients With High-Risk Malignant Salivary Gland Tumors
Postoperative Concurrent Chemoradiotherapy in Treating Patients With High-Risk Malignant Salivary Gland Tumors: *Conditions*: Salivary Gland Tumors; Head and Neck Cancer *Interventions*: Drug: Cisplatin; Drug: Docetaxel; Radiation: Intensity-modulated radiotherapy *Sponsor*: Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University ArmsAssigned Interventions Experimental: Cisplatin Group Intensity-modulated radiotherapy+concurrent chemotherapy with cisplatin for squamous carcinoma of salivary gland Drug: Cisplatin Cisplatin 80mg/m^2，D1-3，2 cycles Oth... περισσότερα »
Treating Patients With Stage III-IV Head and Neck Squamous Cell Carcinoma
Cisplatin, Radiation Therapy, and Pembrolizumab in Treating Patients With Stage III-IV Head and Neck Squamous Cell Carcinoma: This phase I trial studies the side effects and best dose of pembrolizumab when given together with cisplatin and radiation therapy, in treating patients with stage III-IV squamous cell carcinoma of the head and neck. Monoclonal antibodies, such as pembrolizumab, may block tumor growth in different ways by targeting certain cells. Drugs used in chemotherapy, such as cisplatin, work in different ways to stop the growth of tumor cells, either by killing the...περισσότερα »
Nasal Congenital Panfollicular Nevus
Nasal Congenital Panfollicular Nevus: AbstractCongenital nasal midline lesions are rarely observed pathologies. While relatively frequently encountered lesions are dermoid cyst, glioma, encephalocele, nasal polyp and epidermoid cyst, an other very rare reason is the congenital panfollicular nevus. The treatment of this syndrome appearing with a recurrent discharge in clinic is the surgical excision. We will hereby present the excision of the case of congenital panfollicular nevus with three different fistula openings.
Osteoblastoma of the thyroid cartilage
Osteoblastoma de cartílago tiroides via Acta Otorrinolaringológica Española Publication date: Available online 18 May 2016 *Source:*Acta Otorrinolaringológica Española Author(s): Francisco Javier García Callejo, Jaume Redondo Martínez, Verónica Andrade Gamarra, Tomás Pérez Carbonell [image: Corte axial (A) y reconstrucción 3D (B) de la TC cervical, apreciando ...] [image: Imagen microscópica de la lesión nodular bien delimitada por una seudocápsula ...]
Granular cell tumour in the masseter muscle
Tumor de células granulares en músculo masetero via Acta Otorrinolaringológica Española Publication date: Available online 17 May 2016 *Source:*Acta Otorrinolaringológica Española Author(s): María Martel-Martín, David Virós-Porcuna, Rosa D. Ramirez-Ruiz, Irene Moysset-Agusti [image: Resonancia magnética: se observa la tumoración homogénea (*) en el músculo ...] [image: A) Glándula parótida (*) y tumoración localizada en el músculo masetero (**) ...]
Total ossicular replacement prostheses
Análisis experimental mediante vibrometría láser doppler en huesos temporales de cadáver fresco de una nueva prótesis de maleovestibulopexia via Acta Otorrinolaringológica Española Publication date: Available online 17 May 2016 *Source:*Acta Otorrinolaringológica Española Author(s): Luis A. Vallejo, María T. Manzano, Antonio Hidalgo, Alberto Hernández, Juan Sabas, Hugo Lara, Elisa Gil-Carcedo, David Herrero Introducción y objetivosConfirmar en temporales frescos de cadáver el comportamiento óptimo teórico (previamente determinado en un modelo computadorizado del oído medio humano) de un...περισσότερα »
Isolated osteoma of the sphenoid sinus
Isolated osteoma of the sphenoid sinus via Acta Otorrinolaringológica Española Publication date: Available online 18 May 2016 *Source:*Acta Otorrinolaringológica Española Author(s): Ramanuj Sinha, Neeraj Aggarwal, Mainak Dutta [image: Image for figure Figure 1]
Follicular Dendritic Cell Sarcomas
: Clinicopathological Study of 15 Cases with Emphasis on Novel Expression of MDM2, Somatostatin Receptor 2A and PD-L1 via Annals of Diagnostic Pathology Publication date: Available online 18 May 2016 *Source:*Annals of Diagnostic Pathology Author(s): Abbas Agaimy, Michael Michal, Ladislav Hadravsky, Michal Michal Follicular dendritic cell sarcoma (FDCS) is a rare low-grade neoplasm with the phenotype of FDC cells. This rare sarcoma has been well known for being mistaken for a variety of neoplasms (mainly meningioma), particularly at extranodal sites. Diagnosis of FDCS mainly relies on c... περισσότερα »
Salivary epidermal growth factor (EGF) in Sjögren's syndrome
: association between salivary EGF levels and the severity of intraoral manifestations Nihon Rinsho Meneki Gakkai Kaishi. 2016;39(1):42-50 Authors: Azuma N, Katada Y, Kitano S, Nishioka A, Sekiguchi M, Kitano M, Hashimoto N, Matsui K, Iwasaki T, Sano H Abstract Sjögren's syndrome (SS) is a chronic inflammatory autoimmune disease characterized by lymphocytic infiltration of the exocrine glands, especially the salivary and lacrimal glands. As a result of salivary gland dysfunction, most patients with SS have xerostomia, related to a reduced salivary flow rate. In addition to the d...περισσότερα »
Recombinant Vesicular Stomatitis Virus-Based Dengue-2 Vaccine
Candidate Induces Humoral Response and Protects Mice Against Lethal Infection Hum Vaccin Immunother. 2016 May 16;:0 Authors: Lauretti F, Chattopadhyay A, de Oliveira França RF, Castro-Jorge L, Rose J, da Fonseca BA Abstract Dengue is the most important arbovirus disease throughout the world and it is responsible for more than 500,000 dengue hemorrhagic cases and 22,000 deaths every year. One vaccine was recently licensed for human use in Brazil, Mexico and Philippines and although at least seven candidates have been in clinical trials the results of the most developed CYD vaccine...περισσότερα »
Intrathecal hyperbaric versus isobaric bupivacaine for adult non-caesarean-section surgery
systematic review protocol: Introduction Bupivacaine is the most commonly used local anaesthetic for spinal anaesthesia (SA). There are two forms of commercially available bupivacaine; isobaric bupivacaine (IB): a formulation with a specific gravity or density equal to cerebrospinal fluid, and hyperbaric bupivacaine (HB): a formulation with density heavier than cerebrospinal fluid. The difference in densities of the two available preparations is believed to affect the diffusion pattern that determines the effectiveness, spread and side-effect profile of bupivacaine. This systematic ... περισσότερα »
Serotonin-induced contractile responses of esophageal smooth muscle
in the house musk shrew (Suncus murinus): Abstract Background Serotonin (5-hydroxytryptamine, 5-HT) is a regulatory factor in motility of the gastrointestinal tract including the esophagus. Although we proposed that vagal cholinergic and mast cell-derived non-cholinergic components including serotonin coordinately shorten the esophagus, the precise mechanism of serotonin-induced contractions in the suncus esophagus is still unclear. Therefore, the aims of this study were to determine characteristics of contractile responses induced by serotonin and to identify 5-HT receptor subtyp... περισσότερα »
Esophageal acid sensitivity and mucosal integrity in patients with functional heartburn
Esophageal acid sensitivity and mucosal integrity in patients with functional heartburn: Abstract Background Patients with functional heartburn (FH) experience troublesome heartburn that is not related to gastroesophageal reflux. The etiology of the heartburn sensation in FH patients is unknown. In patients with reflux disease, esophageal hypersensitivity seems associated with impaired mucosal integrity. We aimed to determine esophageal sensitivity and mucosal integrity in FH and non-erosive reflux disease (NERD) patients. Methods In this prospective experimental study, we performed... περισσότερα »
Intracerebral fibromas
Intracerebral fibroma: a case report and review of the literature: Intracerebral fibromas are among the most rare neoplasms found in the central nervous system. Ten previously reported cases have been documented in the literature including only two reported cases since 1985. As a result, little is known about these uncommon intracerebral fibrous tumors. We report a case of an intracerebral fibroma without dural or leptomeningeal attachment, discuss the pertinent diagnostic findings and briefly review all prior reports of this entity.
Olfactory Hallucinations and Paresthesias
Case 15-2016: A 32-Year-Old Man with Olfactory Hallucinations and Paresthesias: Presentation of Case. Dr. Ivana Vodopivec (Neurology): A 32-year-old man was seen in an outpatient clinic of this hospital because of olfactory hallucinations, intermittent numbness and paresthesias on the left side, and a mass in the right temporal lobe. The patient had been well until…
Spontaneously active NaV1.5 sodium channels may underlie odor sensitivity
Spontaneously active NaV1.5 sodium channels may underlie odor sensitivity: The olfactory system is remarkably sensitive to airborne odor molecules, but precisely how very low odor concentrations bordering on just a few molecules per olfactory sensory neuron can trigger graded changes in firing is not clear. This report re-examines signaling in olfactory sensory neurons in light of the recent account of NaV1.5 sodium channel-mediated spontaneous firing. Using a model of spontaneous channel activity, the study shows how even sub-millivolt changes in membrane potential elicited by odo...περισσότερα »
Herbs to Cure Stomatitis Recurrent Aphthous Ulcer (RAU)
The Most Common Herbs to Cure the Most Common Oral Disease: Stomatitis Recurrent Aphthous Ulcer (RAU) Iran Red Crescent Med J. 2016 ;18(2):e21694 Authors: Hamedi S, Sadeghpour O, Shamsardekani MR, Amin G, Hajighasemali D, Feyzabadi Z Abstract CONTEXT: Recurrent aphthous ulcer (RAU) is an oral disease and the most common oral lesion, with 2% to 66% of the world's population infected annually. Its prevalence is about 25% in Tehran and 27.6% in Mashhad. The etiology of RAU is multifactorial. Aphthous risk factors include: immunological factors, psychological factors, stress, trauma, ... περισσότερα »
Intraoral administration of botulinum toxin for trigeminal neuropathic pain.
Intraoral administration of botulinum toxin for trigeminal neuropathic pain Oral Surg Oral Med Oral Pathol Oral Radiol. 2016 Jun;121(6):e148-53 Authors: Herrero Babiloni A, Kapos FP, Nixdorf DR Abstract This article presents 2 cases of different neuropathic trigeminal pain conditions treated with intraoral botulinum toxin injections. There is a growing body of evidence to support the use of this substance when administered subcutaneously in the treatment of neuropathic pain, such as in extraoral injections for trigeminal neuralgia. However, reports of intraoral submucosal administr... περισσότερα »
Evaluating cone-beam computed tomography imaging for adenoid hypertrophy screening: a comparison with nasopharyngoscopy.
Accuracy and reliability of oral maxillofacial radiologists when evaluating cone-beam computed tomography imaging for adenoid hypertrophy screening: a comparison with nasopharyngoscopy.: Oral Surg Oral Med Oral Pathol Oral Radiol. 2016 Jun;121(6):e168-74 Authors: Pachêco-Pereira C, Alsufyani NA, Major MP, Flores-Mir C Abstract OBJECTIVE: To determine how accurate and reliable oral maxillofacial radiologists (OMFRs) are in screening for adenoid hypertrophy when using cone-beam computed tomography (CBCT) imaging compared with nasopharyngoscopy (NP). STUDY DESIGN: CBCT scans of 10 pa... περισσότερα »
Can tongue shadow in panoramic radiographs be avoided by using the tongue repositioning maneuver?
Can tongue shadow in panoramic radiographs be avoided by using the tongue repositioning maneuver?: Authors: Cordesmeyer R, Engelke W, Sömmer C, Kauffmann POral Surg Oral Med Oral Pathol Oral Radiol. 2016 Jun;121(6):e175-80 Abstract OBJECTIVE: The purpose of this study was to evaluate the use of the tongue repositioning maneuver (TRM) during panoramic radiography so as to avoid the error of tongue shadow. STUDY DESIGN: A total of 300 panoramic radiographs were evaluated. One hundred and fifty orthopantomograms with conventional positioning technique were used as a control group, w... περισσότερα »
Air Quality in Homes
Associations between Parents’ Perceived Air Quality in Homes and Health among Children in Nanjing, China: by Hua Qian, Xiaohong Zheng, Min Zhang, Louise Weschler, Jan Sundell Abstract The increasing prevalence of respiratory diseases in Chinese children has focused attention on indoor environmental quality. We investigated associations between perceived air quality in domestic environments and children’s allergic diseases with a questionnaire survey study. A total of 4017 children aged 1–8 years old from 23 kindergartens in urban, suburban and industrial areas in Nanjing were ra... περισσότερα »
Pain and paresthesia on the one side of the face
Pterygopalatine fossa metastasis with severe trigeminal neuralgia from neuroendocrine carcinoma of the breast via Auris Nasus Larynx Publication date: Available online 18 May 2016 *Source:*Auris Nasus Larynx Author(s): Taro Takanami, Yasushi Ota, Mitsuya Suzuki [image: Magnetic resonance imaging showing an enhancing soft tissue mass within the ...] [image: Hematoxylin and eosin stains showing that the shape of the tumor cells ranged ...] A 57-year-old woman presented to our department with a 2-month history of pain and paresthesia on the left side of the face 12 years after having unde...περισσότερα »
Megamap: Flexible representation of a large space embedded with nonspatial information by a hippocampal attractor network
AlexAndros SfakianakisστοMedicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026Πριν από 28 δευτερόλεπτα
The problem of how the hippocampus encodes both spatial and nonspatial information at the cellular network level remains largely unresolved. Spatial memory is widely modeled through the theoretical framework of attractor networks, but standard computational models can only represent spaces that are much smaller than the natural habitat of an animal. We propose that hippocampal networks are built upon a basic unit called a megamap, or a cognitive attractor map in which place cells are flexibly recombined to represent a large space. Its inherent flexibility gives the megamap a huge ... περισσότερα »
Prefrontal and Anterior Cingulate Cortex Neurons Encode Attentional Targets Even When They Do Not Apparently Bias Behavior
AlexAndros SfakianakisστοMedicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026Πριν από 28 δευτερόλεπτα
Neurons in anterior cingulate and prefrontal cortex (ACC/PFC) carry information about behaviorally relevant target stimuli. This information is believed to affect behavior by exerting a top-down attentional bias on stimulus selection. However, attention information may not necessarily be a biasing signal, but could be a corollary signal that is not directly related to ongoing behavioral success, or it could reflect the monitoring of targets similar to an eligibility trace useful for later attentional adjustment. To test this suggestion we quantified how attention information relat... περισσότερα »
Spontaneously active NaV1.5 sodium channels may underlie odor sensitivity
AlexAndros SfakianakisστοMedicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026Πριν από 28 δευτερόλεπτα
The olfactory system is remarkably sensitive to airborne odor molecules, but precisely how very low odor concentrations bordering on just a few molecules per olfactory sensory neuron can trigger graded changes in firing is not clear. This report re-examines signaling in olfactory sensory neurons in light of the recent account of NaV1.5 sodium channel-mediated spontaneous firing. Using a model of spontaneous channel activity, the study shows how even sub-millivolt changes in membrane potential elicited by odor are expected to cause meaningful changes in NaV1.5-dependent firing. The...περισσότερα »
Dopamine D1 receptor modulation of calcium channel currents in horizontal cells of mouse retina
AlexAndros SfakianakisστοMedicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026Πριν από 28 δευτερόλεπτα
Horizontal cells form the first laterally interacting network of inhibitory interneurons in the retina. Dopamine, released onto horizontal cells under photic and circadian control modulates horizontal cell function. Using isolated, identified horizontal cells from a connexin-57-iCre x ROSA26-tdTomato transgenic mouse line, we investigated dopaminergic modulation of calcium channel currents (ICa) with whole cell patch-clamp techniques. Dopamine (10 µM) blocked 27% of steady-state ICa, an action blunted to 9% in the presence of the L-type Ca channel blocker verapamil (50 µM). The do... περισσότερα »
Promiscuous and specific bacterial symbiont acquisition in the amoeboid genus Nuclearia (Opisthokonta)
fasoladaellinikiστοOTOLARYNGOLOGY LIFELONG LEARNING : OTOLARYNGOLOGY BY ALEXANDROS SFAKIANAKIS,GREECE CRETE,ANAPAFSEOS 5 AGIOS NIKOLAOS 72100,TEL : 00302841026182,00306932607174Πριν από 31 δευτερόλεπτα
We isolated 17 strains of the amoeboid genus Nuclearia (Opisthokonta) from five Swiss lakes. Eight of these nucleariid isolates were associated with bacterial endo- and /or ectosymbionts. Amoebae were characterized morphologically and by their 18S rRNA genes. Phylogeny based on molecular data resulted in four established monophyletic branches and two new clusters. A heterogeneous picture […]
Role of secondary metabolites in the interaction between Pseudomonas fluorescens and soil microorganisms under iron limited conditions
fasoladaellinikiστοOTOLARYNGOLOGY LIFELONG LEARNING : OTOLARYNGOLOGY BY ALEXANDROS SFAKIANAKIS,GREECE CRETE,ANAPAFSEOS 5 AGIOS NIKOLAOS 72100,TEL : 00302841026182,00306932607174Πριν από 31 δευτερόλεπτα
Microorganisms can be versatile in their interactions with each other, being variously beneficial, neutral or antagonistic in their effect. Although this versatility has been observed among many microorganisms and in many environments, little is known regarding the mechanisms leading to these changes in behavior. In the present work, we analyzed the mechanism by which the […]
Paraburkholderia nodosa is the main N2-fixing species trapped by promiscuous common bean (Phaseolus vulgaris L.) in the Brazilian «Cerradao»
fasoladaellinikiστοOTOLARYNGOLOGY LIFELONG LEARNING : OTOLARYNGOLOGY BY ALEXANDROS SFAKIANAKIS,GREECE CRETE,ANAPAFSEOS 5 AGIOS NIKOLAOS 72100,TEL : 00302841026182,00306932607174Πριν από 31 δευτερόλεπτα
The bacterial genus Burkholderia comprises species occupying several habitats, including a group of symbionts of leguminous plants—also called beta-rhizobia—that has been recently ascribed to the new genus Paraburkholderia. We used common bean (Phaseolus vulgaris L.) plants to trap rhizobia from an undisturbed soil of the Brazilian Cerrado under the vegetation type «Cerradão». Genetic characterization started […]
Working memory-driven attention improves spatial resolution: Support for perceptual enhancement
fasoladaellinikiστοOTOLARYNGOLOGY LIFELONG LEARNING : OTOLARYNGOLOGY BY ALEXANDROS SFAKIANAKIS,GREECE CRETE,ANAPAFSEOS 5 AGIOS NIKOLAOS 72100,TEL : 00302841026182,00306932607174Πριν από 31 δευτερόλεπτα
Abstract Previous research has indicated that attention can be biased toward those stimuli matching the contents of working memory and thereby facilitates visual processing at the location of the memory-matching stimuli. However, whether this working memory-driven attentional modulation takes place on early perceptual processes remains unclear. Our present results showed that working memory-driven attention improved […]
A longitudinal analysis of chronic arm morbidity following breast cancer surgery
fasoladaellinikiστοOTOLARYNGOLOGY LIFELONG LEARNING : OTOLARYNGOLOGY BY ALEXANDROS SFAKIANAKIS,GREECE CRETE,ANAPAFSEOS 5 AGIOS NIKOLAOS 72100,TEL : 00302841026182,00306932607174Πριν από 31 δευτερόλεπτα
Abstract Arm morbidity (AM) arising from breast cancer (BC) treatment can detrimentally impact quality of life; often limiting a survivor’s ability to participate in valued activities. The present study explored (a) the developmental time course of AM [restricted range of motion (ROM), pain, and arm volume changes], negative affect, and perceived disability in the immediate […]
When is it time to move to the next map? Optimal foraging in guided visual search
fasoladaellinikiστοOTOLARYNGOLOGY LIFELONG LEARNING : OTOLARYNGOLOGY BY ALEXANDROS SFAKIANAKIS,GREECE CRETE,ANAPAFSEOS 5 AGIOS NIKOLAOS 72100,TEL : 00302841026182,00306932607174Πριν από 31 δευτερόλεπτα
Abstract Suppose that you are looking for visual targets in a set of images, each containing an unknown number of targets. How do you perform that search, and how do you decide when to move from the current image to the next? Optimal foraging theory predicts that foragers should leave the current image when the […]
Targeting cAMP/PKA signaling pathway for glycemic control and type 2 diabetes therapy
fasoladaellinikiστοOTOLARYNGOLOGY LIFELONG LEARNING : OTOLARYNGOLOGY BY ALEXANDROS SFAKIANAKIS,GREECE CRETE,ANAPAFSEOS 5 AGIOS NIKOLAOS 72100,TEL : 00302841026182,00306932607174Πριν από 31 δευτερόλεπτα
In mammals, cyclic adenosine monophosphate (cAMP) is an intracellular second messenger that is usually elicited by binding of hormones and neurotransmitters to G protein-coupled receptors (GPCRs). cAMP exerts many of its physiological effects by activating cAMP-dependent protein kinase (PKA), which in turn phosphorylates and regulates the functions of downstream protein targets including ion channels, enzymes, […]
Face mask and skeletal anchorage therapy with intermaxillary elastics in patients with maxillary retrognathia
fasoladaellinikiστοOTOLARYNGOLOGY LIFELONG LEARNING : OTOLARYNGOLOGY BY ALEXANDROS SFAKIANAKIS,GREECE CRETE,ANAPAFSEOS 5 AGIOS NIKOLAOS 72100,TEL : 00302841026182,00306932607174Πριν από 31 δευτερόλεπτα
Comparison of short-term effects between Introduction: The aim of this study was to compare the short-term dental and skeletal effects of a face mask (FM) with those of skeletal anchorage (SA) therapy with intermaxillary elastics in prepubertal patients with skeletal Class III malocclusion. Methods: Fifty patients with skeletal Class III malocclusion and maxillary deficiency were […]
Azithromycin therapy during RSV bronchiolitis: upper airway microbiome alterations and subsequent recurrent wheeze
fasoladaellinikiστοOTOLARYNGOLOGY LIFELONG LEARNING : OTOLARYNGOLOGY BY ALEXANDROS SFAKIANAKIS,GREECE CRETE,ANAPAFSEOS 5 AGIOS NIKOLAOS 72100,TEL : 00302841026182,00306932607174Πριν από 31 δευτερόλεπτα
(Source: Journal of Allergy and Clinical Immunology) from #Medicine-SfakianakisAlexandros via o.lakala70 on Inoreader http://ift.tt/1ToPxcR via IFTTT
Orofacial dysfunction, open bite, and myofunctional therapy
fasoladaellinikiστοOTOLARYNGOLOGY LIFELONG LEARNING : OTOLARYNGOLOGY BY ALEXANDROS SFAKIANAKIS,GREECE CRETE,ANAPAFSEOS 5 AGIOS NIKOLAOS 72100,TEL : 00302841026182,00306932607174Πριν από 31 δευτερόλεπτα
Orofacial dysfunction, open bite, and myofunctional therapy: Many orthodontists see open bites as their most demanding assignments; aesthetic issues must be taken into account, the treatment is difficult and the long-term stability unpredictable. Myofunctional treatment may not always be the right choice for this category of malocclusions, but it should be given a serious consideration. […]
Alloplastic total temporomandibular joint replacements: do they perform like natural joints? Prospective cohort study with a historical control
sfakianakismedicsστοORL ALLERGY AESTHETICS ANATOMY Medicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174Πριν από 35 δευτερόλεπτα
Publication date: Available online 19 May 2016 Source:International Journal of Oral and Maxillofacial Surgery Author(s): A. Wojczyńska, C.S. Leiggener, M. Bredell, D.A. Ettlin, S. Erni, L.M. Gallo, V. Colombo The aim of this study was to qualitatively and quantitatively describe the biomechanics of existing total alloplastic reconstructions of temporomandibular joints (TMJ). Fifteen patients with unilateral […]
Azithromycin therapy during RSV bronchiolitis: upper airway microbiome alterations and subsequent recurrent wheeze
(Source: Journal of Allergy and Clinical Immunology) from #Head and Neck by Sfakianakis via simeraentaxei on Inoreader http://ift.tt/1ToPxcR via IFTTT
Follicular Helper T (Tfh) Cells Mediate IgE Antibody Response to Airborne Allergens
Using mouse models, Kobayashi et al. show that IL-4 producing Tfh cells develop following airway exposure to natural allergens, and that the Tfh cells are necessary and sufficient for sustained production of IgE antibody. (Source: Journal of Allergy and Clinical Immunology) from #Head and Neck by Sfakianakis via simeraentaxei on Inoreader http://ift.tt/1TqjfPV via IFTTT
Alloplastic total temporomandibular joint replacements: do they perform like natural joints? Prospective cohort study with a historical control
Publication date: Available online 19 May 2016 Source:International Journal of Oral and Maxillofacial Surgery Author(s): A. Wojczyńska, C.S. Leiggener, M. Bredell, D.A. Ettlin, S. Erni, L.M. Gallo, V. Colombo The aim of this study was to qualitatively and quantitatively describe the biomechanics of existing total alloplastic reconstructions of temporomandibular joints (TMJ). Fifteen patients with unilateral… Continue reading Alloplastic total temporomandibular joint replacements: do they perform like natural joints? Prospective cohort study with a historical control
Targeting cAMP/PKA signaling pathway for glycemic control and type 2 diabetes therapy
In mammals, cyclic adenosine monophosphate (cAMP) is an intracellular second messenger that is usually elicited by binding of hormones and neurotransmitters to G protein-coupled receptors (GPCRs). cAMP exerts many of its physiological effects by activating cAMP-dependent protein kinase (PKA), which in turn phosphorylates and regulates the functions of downstream protein targets including ion channels, enzymes,… Continue reading Targeting cAMP/PKA signaling pathway for glycemic control and type 2 diabetes therapy
Survival After Percutaneous Endoscopic Gastrostomy in Older Adults With Neurologic Disorders
Background: Percutaneous endoscopic gastrostomy (PEG) tube placement is a common procedure performed to provide a route for enteral feeding. We aimed to examine the survival after PEG in older adults with neurologic disease and to demonstrate the factors associated with mortality. Methods: We analyzed the data of 500 patients who underwent PEG placement between 2005… Continue reading Survival After Percutaneous Endoscopic Gastrostomy in Older Adults With Neurologic Disorders
Substrate stiffness orchestrates epithelial cellular heterogeneity with controlled proliferative pattern via E-cadherin/β-catenin mechanotransduction
Publication date: Available online 18 May 2016 Source:Acta Biomaterialia Author(s): Bingjie Wang, Peng Qin, Hui Zhao, Tie Xia, Jingyu Wang, Longwei Liu, Lu Zhu, Jing Xu, Chenyu Huang, Yan Shi, Yanan Du Epithelial cellular heterogeneity has been observed in pathological tissues with abnormal matrix stiffness and cells cultured on rigid substrates. However, it remains unclear… Continue reading Substrate stiffness orchestrates epithelial cellular heterogeneity with controlled proliferative pattern via E-cadherin/β-catenin mechanotransduction
Collagen-collagen interactions mediated by plant-derived proanthocyanidins: a spectroscopic and atomic force microscopy study
Publication date: Available online 18 May 2016 Source:Acta Biomaterialia Author(s): Cristina M.P. Vidal, Weiying Zhu, Suresh Manohar, Berdan Aydin, Timothy A. Keiderling, Phillip B. Messersmith, Ana Bedran-Russo Collagen cross-linkings are determinant of biological tissue stability and function. Plant-derived proanthocyanidins (PACs) mimic different hierarchical levels of collagen cross-links by non-enzymatic interactions resulting in the enhancement to… Continue reading Collagen-collagen interactions mediated by plant-derived proanthocyanidins: a spectroscopic and atomic force micr... περισσότερα »
A Monoclonal Antibody That Discriminates Between SNAP-Tagged and CLIP-Tagged Proteins.
In this study, we describe a new mAb, which is specific for the SNAP-tag alone. Therefore, this mAb allows discrimination between SNAP- and CLIP-tags within a broad range of immunological methods, including enzyme-linked immunosorbent assays, western blotting, flow cytometry, and immunohistochemistry. PMID: 27187007 [PubMed – as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and… Continue reading A Monoclonal Antibody That Discriminates Between SNAP-Tagged and CLIP-Tagged Proteins.
Investigation of endocrine and immunological response in fat tissue to hyperbaric oxygen administration in rats.
In conclusion, in our study we identified that HBO administration affected the endochrinological functions of fat tissue. PMID: 27188864 [PubMed – in process] (Source: Cellular and Molecular Biology) from #Head and Neck by Sfakianakis via simeraentaxei on Inoreader http://ift.tt/1R9WV7m via IFTTT
Disruption of a Regulatory Network Consisting of Neutrophils and Platelets Fosters Persisting Inflammation in Rheumatic Diseases
Norma Maugeri, Patrizia Rovere-Querini, Angelo A. Manfredi (Source: Frontiers in Immunology) from #Head and Neck by Sfakianakis via simeraentaxei on Inoreader http://ift.tt/1XBk2iG via IFTTT
Esophageal acid sensitivity and mucosal integrity in patients with functional heartburn
Abstract Background Patients with functional heartburn (FH) experience troublesome heartburn that is not related to gastroesophageal reflux. The etiology of the heartburn sensation in FH patients is unknown. In patients with reflux disease, esophageal hypersensitivity seems associated with impaired mucosal integrity. We aimed to determine esophageal sensitivity and mucosal integrity in FH and non-erosive reflux disease (NERD) patients. Methods In this prospective experimental study, we performed an acid perfusion test and upper endoscopy with biopsies in 12 patients with NERD and ni... περισσότερα »
Serotonin-induced contractile responses of esophageal smooth muscle in the house musk shrew (Suncus murinus)
Abstract Background Serotonin (5-hydroxytryptamine, 5-HT) is a regulatory factor in motility of the gastrointestinal tract including the esophagus. Although we proposed that vagal cholinergic and mast cell-derived non-cholinergic components including serotonin coordinately shorten the esophagus, the precise mechanism of serotonin-induced contractions in the suncus esophagus is still unclear. Therefore, the aims of this study were to determine characteristics of contractile responses induced by serotonin and to identify 5-HT receptor subtypes responsible for regulating motility in th...περισσότερα »
Working memory-driven attention improves spatial resolution: Support for perceptual enhancement
Abstract Previous research has indicated that attention can be biased toward those stimuli matching the contents of working memory and thereby facilitates visual processing at the location of the memory-matching stimuli. However, whether this working memory-driven attentional modulation takes place on early perceptual processes remains unclear. Our present results showed that working memory-driven attention improved identification of a brief Landolt target presented alone in the visual field. Because the suprathreshold target appeared without any external noise added (i.e., no dist... περισσότερα »
When is it time to move to the next map? Optimal foraging in guided visual search
Abstract Suppose that you are looking for visual targets in a set of images, each containing an unknown number of targets. How do you perform that search, and how do you decide when to move from the current image to the next? Optimal foraging theory predicts that foragers should leave the current image when the expected value from staying falls below the expected value from leaving. Here, we describe how to apply these models to more complex tasks, like search for objects in natural scenes where people have prior beliefs about the number and locations of targets in each image, and ... περισσότερα »
Interfering lncRNA consumes OncomiRs to inhibit HCC
The endogenous microRNAs (miRNAs), especially the oncogenic miRNAs (OncomiRs), have been molecular targets for cancer therapy. We generated an artificially-designed interfering long non-coding RNA (lncRNAi), which contains the sequences that can complementarily bind to multiple OncomiRs and is expressed by cancer-selectively replicating adenovirus. The adenovirus-expressed lncRNAi with high level in hepatocellular carcinoma (HCC) cells competes with OncomiR target genes to bind to and consume OncomiRs, thereby achieving the targeted anti-HCC efficacy. With the targeting replicatio... περισσότερα »
Unblocking the cancer drug development bottleneck via innovative partnering
Future Oncology Ahead of Print. from Cancer via ola Kala on Inoreader http://ift.tt/251juot via IFTTT from #Med Blogs by Alexandros G.Sfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/1WENFQA via IFTTT
Sonidegib for the treatment of advanced basal cell carcinoma: a comprehensive review of sonidegib and the BOLT trial with 12-month update
Future Oncology Ahead of Print. from Cancer via ola Kala on Inoreader http://ift.tt/27C1N14 via IFTTT from #Med Blogs by Alexandros G.Sfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/1qvsZwu via IFTTT
Specific immunotherapy for rhinitis and asthma with a subcutaneous hypoallergenic high-dose house dust mite extract: results of a 9-month therapy
Immunotherapy Ahead of Print. from #Medicine-Sfakianakis via simeraentaxei on Inoreader http://ift.tt/1TItnQd via IFTTT
A novel adsorbent TEMPO-mediated oxidized cellulose nanofibrils modified with PEI: Preparation, characterization, and application for Cu(II) removal
Publication date: 5 October 2016 Source:Journal of Hazardous Materials, Volume 316 Author(s): Nan Zhang, Guo-Long Zang, Chen Shi, Han-Qing Yu, Guo-Ping Sheng This study describes the preparation of a novel adsorbent based on cellulose nanofibrils by first TEMPO mediated oxidation and then PEI grafting (TOCN-PEI) for heavy metal removal. FTIR results demonstrated the successful introduction… Continue reading A novel adsorbent TEMPO-mediated oxidized cellulose nanofibrils modified with PEI: Preparation, characterization, and application for Cu(II) removal
Quantification and human health risk assessment of by-products of photo catalytic oxidation of ethylbenzene, xylene and toluene in indoor air of analytical laboratories
Publication date: 5 October 2016 Source:Journal of Hazardous Materials, Volume 316 Author(s): Indramani Dhada, Mukesh Sharma, Pavan Kumar Nagar The by-products of TiO2-based photocatalytic oxidation (PCO) of ethylbenze, p,m-xylene, o-xylene and toluene (EXT) in vapour phase and those adsorbed on the catalyst surface (solid phase) were identified and quantified on GC/GC–MS. A factor was developed… Continue reading Quantification and human health risk assessment of by-products of photo catalytic oxidation of ethylbenzene, xylene and toluene in indoor air of analytical laboratories
Comprehensive insights into microcystin-LR effects on hepatic lipid metabolism using cross-omics technologies
Publication date: 5 September 2016 Source:Journal of Hazardous Materials, Volume 315 Author(s): Zongyao Zhang, Xu-Xiang Zhang, Bing Wu, Jinbao Yin, Yunjiang Yu, Liuyan Yang Microcystin-LR (MC-LR) can induce hepatic tissue damages and molecular toxicities, but its effects on lipid metabolism remain unknown. This study investigated the effects of MC-LR exposure on mice lipid metabolism and… Continue reading Comprehensive insights into microcystin-LR effects on hepatic lipid metabolism using cross-omics technologies
Demonstration of Autosomal Monoallelic Expression in Thyroid Tissue Assessed by Whole-Exome and Bulk RNA Sequencing
Thyroid , Vol. 0, No. 0. from #Head and Neck by Sfakianakis via simeraentaxei on Inoreader http://ift.tt/1Tqe8iI via IFTTT
Ridge preservation of extraction sockets with chronic pathology using Bio-Oss® Collagen with or without collagen membrane: an experimental study in dogs
Abstract Objectives This study aimed to evaluate the dynamics of newly bone formation and dimensional change in diseased extraction sockets using Bio-Oss® Collagen with or without a collagen membrane. Material and methods In six beagle dogs, right and left 3rd and 4th mandibular premolars were hemisected and the distal roots were removed. Combined endodontic–periodontic lesions… Continue reading Ridge preservation of extraction sockets with chronic pathology using Bio-Oss® Collagen with or without collagen membrane: an experimental study in dogs
GSE81609 Plasma Cell Niche
Contributors : Julia M Shaw ; Marjorie Robert-Guroff Series Type : Expression profiling by array Organism : Macaca mulatta Sorted cells from bone marrow and rectal mucosa of SIV-infected rhesus macaques were analyzed for expression of factors associated with plasma cell recruitment, adhesion, or maintenance mRNA expression anaylsis was performed on 16 CD2-CD19-CD20-HLA-DR+ and 16… Continue reading GSE81609 Plasma Cell Niche
GSE81586 Impact of transposable elements in Pleurotus ostreatus transcriptome profiles
Contributors : Lucía Ramírez ; Raúl Castanera Series Type : Expression profiling by high throughput sequencing Organism : Pleurotus ostreatus Pleurotus ostreatus, also known as the oyster mushroom, is an active lignin decomposer in the forests. The genomes of the monokaryotic strains PC15 and PC9 have been used to characterize the content and distribution of… Continue reading GSE81586 Impact of transposable elements in Pleurotus ostreatus transcriptome profiles
GSE80832 Human U937 cell transcriptome response to Zaire Ebola virus wild-type in the ΔVP30 background and Δmucin virus mRNA
Contributors : Pete Halfmann ; Allison Thompson ; Katrina M Waters ; Amie J Eisfeld ; Yoshihiro Kawaoka Series Type : Expression profiling by array Organism : Homo sapiens The purpose is to obtain samples for mRNA analysis in human U937 cells infected with Zaire Ebola virus wild-type in the ΔVP30 background and Δmucin virus.… Continue reading GSE80832 Human U937 cell transcriptome response to Zaire Ebola virus wild-type in the ΔVP30 background and Δmucin virus mRNA
GSE81579 Transcriptome analysis of human cumulus cells reveals hypoxia as the main determinant of follicular senescence.
Contributors : Emanuela Molinari ; Haim Bar ; Anna Marie Pyle ; Pasquale Patrizio Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens RNA sequencing profiling of cumulus cells revealed that the follicles of older women have a compromised environment with hypoxic stress being the main contributor to age-related oocyte quality… Continue reading GSE81579 Transcriptome analysis of human cumulus cells reveals hypoxia as the main determinant of follicular senescence.
Ridge preservation of extraction sockets with chronic pathology using Bio-Oss® Collagen with or without collagen membrane: an experimental study in dogs
[image: cover.gif?v=1&s=705296a249cff94ae911375e] Abstract Objectives This study aimed to evaluate the dynamics of newly bone formation and dimensional change in diseased extraction sockets using Bio-Oss® Collagen with or without a collagen membrane. Material and methods In six beagle dogs, right and left 3rd and 4th mandibular premolars were hemisected and the distal roots were removed. Combined endodontic–periodontic lesions were induced in all sites using black silk, collagen sponge, endodontic files, and application of *Porphyromonas gingivalis*. After 4 months, among 4 premol... περισσότερα »
Irritable bowel syndrome and the perinatal period: lower birth weight increases the risk
Abstract Background Early life events have been found to be associated with irritable bowel syndrome (IBS) suggesting a role in development of functional disorders. The study aim was to identify potential perinatal risk factors for adult IBS. Methods Utilizing a population-based nested case–control design, cases who met modified Rome III criteria for IBS and age- and-gender matched controls were identified using responses from prior mailed surveys to a random sample of Olmsted County residents. Medical records of eligible respondents were reviewed for perinatal events of interest. ... περισσότερα »
Demonstration of Autosomal Monoallelic Expression in Thyroid Tissue Assessed by Whole-Exome and Bulk RNA Sequencing
medicinesfakianakisστοMedicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174Πριν από 1 ώρα
Thyroid , Vol. 0, No. 0. from #Medicine-SfakianakisAlexandros via o.lakala70 on Inoreader http://ift.tt/1TqcwWj via IFTTT Medicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174
Radiofrequency ablation
medicinesfakianakisστοMedicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174Πριν από 1 ώρα
Radiofrequency ablation: The use of electrodes to generate heat and destroy abnormal tissue. In radiofrequency ablation (RFA), heat is generated locally by a high frequency, alternating current that flows from the electrodes. A probe is inserted into the center of the tumor and the non-insulated electrodes, which are shaped like prongs, are projected into the […]
Evidence for potential involvement of pro-inflammatory adipokines in the pathogenesis of idiopathic intracranial hypertension
medicinesfakianakisστοMedicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174Πριν από 1 ώρα
Background Although specific role players are currently unknown, contribution of inflammatory mediators has been suggested in the pathophysiology of idiopathic intracranial hypertension (IIH), which is a disease more prevalent in obese female individuals of childbearing age. We aimed to investigate the levels of adipokines and cytokines to demonstrate possible markers for inflammation that participate in […]
Endovascular Repair of a Thoracic Aortic Transection 31 Years after Blunt Trauma
medicinesfakianakisστοMedicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174Πριν από 1 ώρα
from #Medicine-SfakianakisAlexandros via o.lakala70 on Inoreader http://ift.tt/23XTTKR via IFTTT Medicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174
After-pulsing, cross-talk, dark-count, and gain of MPPC under 7-T static magnetic field
medicinesfakianakisστοMedicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174Πριν από 1 ώρα
Abstract Multi-pixel photon counters (MPPCs) have been used instead of photomultiplier tubes for positron emission tomography combined with magnetic resonance (PET-MR). However, the effects of the magnetic field (MF) on the intrinsic properties—gain, cross-talk, after-pulsing, and dark-count—have not been sufficiently investigated. Therefore, we measured these properties for two types of MPPCs—S10931-50P and S12572-50P—in a static […]
Biochemical Classification of Disease-Associated Mutants of RAS-Like Protein Expressed in Many Tissues (RIT1) Protein Structure and Folding
medicinesfakianakisστοMedicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174Πριν από 1 ώρα
RAS-like protein expressed in many tissues 1 (RIT1) is a disease-associated RAS-subfamily small guanosine triphosphatase (GTPase). Recent studies revealed that germ-line and somatic RIT1 mutations can cause Noonan Syndrome (NS), and drive proliferation of lung adenocarcinomas, respectively, akin to RAS mutations in these diseases. However, the locations of these RIT1 mutations differ significantly from those […]
Insights into lysine-deacetylation of natively folded substrate proteins by sirtuins Molecular Biophysics
medicinesfakianakisστοMedicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174Πριν από 1 ώρα
Sirtuins are NAD+ dependent lysine-deacylases, regulating a variety of cellular processes. The nuclear Sirt1, the cytosolic Sirt2 and the mitochondrial Sirt3 are robust deacetylases, whereas the other sirtuins have preferences for longer acylchains. Most previous studies investigated sirtuin-catalysed deacylation on peptide substrates only. We used the genetic code expansion concept to produce natively folded site-specifically […]
Crystal structures of the SpoIID lytic transglycosylases essential for bacterial sporulation Enzymology
medicinesfakianakisστοMedicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174Πριν από 1 ώρα
Bacterial spores are the most resistant form of life known on the Earth and represent a serious problem for i) bioterrorism attack, ii) horizontal transmission of microbial pathogens in the community and iii) persistence in patients and in a nosocomial environment. SpoIID (Stage II Sporulation Protein D) is a Lytic Transglycosylase (LT) essential for sporulation. […]
New insight in the catalytic mechanism of bacterial MraY from enzyme kinetics and docking studies Protein Structure and Folding
medicinesfakianakisστοMedicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174Πριν από 1 ώρα
Phospho-MurNAc-pentapeptide translocase (MraY) catalyzes the synthesis of Lipid I, a bacterial peptidoglycan precursor. As such, MraY is essential for bacterial survival and therefore is an ideal target for developing novel antibiotics. However the understanding of its catalytic mechanism, despite the recently determined crystal structure, remains limited. In the present study, the kinetic properties of Bacillus […]
RNA Polymerase II Trigger Loop Mobility: Indirect Effects of Rpb9 Gene Regulation
medicinesfakianakisστοMedicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174Πριν από 1 ώρα
Rpb9 is a conserved RNA polymerase II (pol II) subunit, the absence of which confers alterations to pol II enzymatic properties and transcription fidelity. It has been suggested previously that Rpb9 affects mobility of the trigger loop (TL), a structural element of Rpb1 that moves in and out of the active site with each elongation […]
Biochemical Classification of Disease-Associated Mutants of RAS-Like Protein Expressed in Many Tissues (RIT1) Protein Structure and Folding
RAS-like protein expressed in many tissues 1 (RIT1) is a disease-associated RAS-subfamily small guanosine triphosphatase (GTPase). Recent studies revealed that germ-line and somatic RIT1 mutations can cause Noonan Syndrome (NS), and drive proliferation of lung adenocarcinomas, respectively, akin to RAS mutations in these diseases. However, the locations of these RIT1 mutations differ significantly from those… Continue reading Biochemical Classification of Disease-Associated Mutants of RAS-Like Protein Expressed in Many Tissues (RIT1) Protein Structure and Folding
Insights into lysine-deacetylation of natively folded substrate proteins by sirtuins Molecular Biophysics
Sirtuins are NAD+ dependent lysine-deacylases, regulating a variety of cellular processes. The nuclear Sirt1, the cytosolic Sirt2 and the mitochondrial Sirt3 are robust deacetylases, whereas the other sirtuins have preferences for longer acylchains. Most previous studies investigated sirtuin-catalysed deacylation on peptide substrates only. We used the genetic code expansion concept to produce natively folded site-specifically… Continue reading Insights into lysine-deacetylation of natively folded substrate proteins by sirtuins Molecular Biophysics
Crystal structures of the SpoIID lytic transglycosylases essential for bacterial sporulation Enzymology
Bacterial spores are the most resistant form of life known on the Earth and represent a serious problem for i) bioterrorism attack, ii) horizontal transmission of microbial pathogens in the community and iii) persistence in patients and in a nosocomial environment. SpoIID (Stage II Sporulation Protein D) is a Lytic Transglycosylase (LT) essential for sporulation.… Continue reading Crystal structures of the SpoIID lytic transglycosylases essential for bacterial sporulation Enzymology
New insight in the catalytic mechanism of bacterial MraY from enzyme kinetics and docking studies Protein Structure and Folding
Phospho-MurNAc-pentapeptide translocase (MraY) catalyzes the synthesis of Lipid I, a bacterial peptidoglycan precursor. As such, MraY is essential for bacterial survival and therefore is an ideal target for developing novel antibiotics. However the understanding of its catalytic mechanism, despite the recently determined crystal structure, remains limited. In the present study, the kinetic properties of Bacillus… Continue reading New insight in the catalytic mechanism of bacterial MraY from enzyme kinetics and docking studies Protein Structure and Folding
RNA Polymerase II Trigger Loop Mobility: Indirect Effects of Rpb9 Gene Regulation
Rpb9 is a conserved RNA polymerase II (pol II) subunit, the absence of which confers alterations to pol II enzymatic properties and transcription fidelity. It has been suggested previously that Rpb9 affects mobility of the trigger loop (TL), a structural element of Rpb1 that moves in and out of the active site with each elongation… Continue reading RNA Polymerase II Trigger Loop Mobility: Indirect Effects of Rpb9 Gene Regulation
Ajuba Phosphorylation by CDK1 Promotes Cell Proliferation and Tumorigenesis Cell Biology
Recent studies identified the adaptor protein Ajuba as a positive regulator of Yes-associated protein (YAP) oncogenic activity through inhibiting large tumor suppressor (Lats1/2) core kinases of the Hippo pathway, a signaling pathway that plays important roles in cancer. In this study, we define a novel mechanism for phospho-regulation of Ajuba in mitosis and its biological… Continue reading Ajuba Phosphorylation by CDK1 Promotes Cell Proliferation and Tumorigenesis Cell Biology
Sulfur denitrosylation by an engineered Trx-like DsbG enzyme identifies nucleophilic cysteine hydrogen bonds as key functional determinant Enzymology
Exposure of bacteria to nitric oxide (NO) results in the nitrosylation of cysteine thiols in proteins and low molecular weight thiols such as glutathione (GSH). Cells possess enzymatic systems that catalyze the denitrosylation of these modified sulfurs. An important player in these systems is thioredoxin (Trx), a ubiquitous, cytoplasmic oxidoreductase that can denitrosylate proteins in… Continue reading Sulfur denitrosylation by an engineered Trx-like DsbG enzyme identifies nucleophilic cysteine hydrogen bonds as key functional determinant Enzymology
NDUFAF5 hydroxylates NDUFS7 at an early stage in the assembly of human complex I Bioenergetics
Complex I (NADH ubiquinone oxidoreductase) in mammalian mitochondria is an L-shaped assembly of 45 proteins. One arm lies in the inner membrane and the other extends about 100 Å into the matrix of the organelle. The extrinsic arm contains binding sites for NADH, the primary electron acceptor FMN, and seven iron-sulfur clusters that form a… Continue reading NDUFAF5 hydroxylates NDUFS7 at an early stage in the assembly of human complex I Bioenergetics
Investigating the Mechanism by which Gain-of-Function Mutations to the {alpha}1 Glycine Receptor cause Hyperekplexia Neurobiology
Hyperekplexia is a rare human neuromotor disorder caused by mutations that impair the efficacy of glycinergic inhibitory neurotransmission. Loss-of-function mutations in the GLRA1 or GLRB genes, which encode the α1 and β glycine receptor (GlyR) subunits, are the major cause. Paradoxically, gain-of-function GLRA1 mutations also cause hyperekplexia, although the mechanism is unknown. Here we identify… Continue reading Investigating the Mechanism by which Gain-of-Function Mutations to the {alpha}1 Glycine Receptor cause Hyperekplexia Neurobiology
Functional Characterization of the Odorant Receptor 51E2 in Human Melanocytes Signal Transduction
Olfactory receptors, which belong to the family of G-Protein coupled receptors, are found to be ectopically expressed in non-sensory tissues mediating a variety of cellular functions. In this study, we detected the olfactory receptor OR51E2 at the transcript and protein level in human epidermal melanocytes. Stimulation of primary melanocytes with the OR51E2 ligand beta-ionone significantly… Continue reading Functional Characterization of the Odorant Receptor 51E2 in Human Melanocytes Signal Transduction
Prospective assessment of serum periostin as a biomarker for diagnosis and monitoring of eosinophilic oesophagitis
Summary Background Periostin is highly expressed in eosinophilic oesophagitis (EoE), but has not been extensively studied as a non-invasive biomarker. Aim To assess whether serum periostin distinguished EoE from controls at baseline, had utility for monitoring treatment response, or was associated with IL-13 levels. Methods This was a sub-analysis of a prospective cohort study of… Continue reading Prospective assessment of serum periostin as a biomarker for diagnosis and monitoring of eosinophilic oesophagitis
Systematic review with meta-analysis: the effect of tobacco smoking on the natural history of ulcerative colitis
Summary Background Tobacco smoking is associated with a reduced risk of developing ulcerative colitis (UC). A high proportion of UC patients perceive a benefit in disease outcomes secondary to smoking. However, the effects of smoking on the natural history of UC are uncertain. Aim To conduct a systematic review and meta-analysis of the effects of… Continue reading Systematic review with meta-analysis: the effect of tobacco smoking on the natural history of ulcerative colitis
An Examination of Sources of Variability Across the Consonant-Nucleus-Consonant Test in Cochlear Implant Listeners
The 10 consonant-nucleus-consonant (CNC) word lists are considered the gold standard in the testing of cochlear implant (CI) users. However, variance in scores across lists could degrade the sensitivity and reliability of them to identify deficits in speech perception. This study examined the relationship between variability in performance among lists and the lexical characteristics of… Continue reading An Examination of Sources of Variability Across the Consonant-Nucleus-Consonant Test in Cochlear Implant Listeners
