Background: There is paucity of literature on the prognostic value of tissue oxygen saturation (StO2) and regional cerebral oxygen saturation (rSO2) in neurological patients with sepsis. In this preliminary study, we investigated the prognostic value of StO2 and rSO2 in a group of neurological patients and correlated StO2 and rSO2 with hemodynamic and metabolic parameters. Materials and Methods: This preliminary, prospective observational study was conducted in 45 adult neurological patients admitted to intensive care unit. Once a diagnosis of sepsis or septic shock was established, parameters of oxygenation (StO2, rSO2, central venous oxygen saturation [ScvO2]), serum lactate, illness severity scores (Acute Physiology and Chronic Health Evaluation score, Sequential Organ Failure Assessment score, Glasgow Coma Scale) were recorded at 0, 6, 12, 24, 36, and 48 hours, and once daily thereafter. Outcomes were in-hospital mortality attributable to sepsis and the Glasgow outcome score at hospital discharge. Results: There was a moderately positive correlation between StO2 and rSO2 at baseline (r=0.599; P=0.001). StO2, illness severity scores and serum lactate, but not rSO2, were significantly different between survivors (n=29) and nonsurvivors (n=16) at baseline and during the first 48 hours. An rSO2 of 62.5% had a sensitivity of 83% and specificity of 67% to differentiate survivors and nonsurvivors of septic shock at 48 hours. StO2 had a higher correlation with ScvO2 and serum lactate than rSO2. Conclusions: StO2 prognosticates survival and favorable/unfavorable outcomes in neurological patients with sepsis. The role of rSO2 in predicting survival in milder form of sepsis is doubtful. The authors have no funding or conflicts of interest to disclose. Address correspondence to: Sonia Bansal, MD, DNB, PDF, Department of Neuroanaesthesia and Neurocritical Care, National Institute of Mental Health and Neurosciences (NIMHANS), Bangalore 560029, India (e-mail: itz.sonia77@gmail.com). Received February 13, 2018 Accepted October 4, 2018 Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved
https://ift.tt/2zpqaVr
Τετάρτη 21 Νοεμβρίου 2018
Predictive Factors for Hypotension Associated With Supine-to-Prone Positional Change in Patients Undergoing Spine Surgery
Background: Positional change during general anesthesia can cause hypotension. The objective of this retrospective study was to determine predictive factors for hypotension associated with supine-to-prone positional change in spinal surgery patients. Materials and Methods: Data on demographics, current medications, comorbidity, intraoperative mean arterial pressure (MAP), heart rate, pulse pressure variation, tidal volume, peak inspiratory pressure, and propofol and remifentanil effect-site concentrations were collected from 179 patients undergoing elective spine surgery. Hypotension associated with supine-to-prone positional change was defined as >20% reduction in MAP during positional change. Results: Hypotension associated with supine-to-prone positional change was observed in 16 (8.9%) patients. The median (interquartile range) effect-site concentration of remifentanil (5.3 [4.0 to 8.5] vs. 4.0 [3.1 to 4.0] ng/mL, P
https://ift.tt/2OZnaUV
https://ift.tt/2OZnaUV
Prevalence of allergic and nonallergic rhinitis in a rural area of northern China based on sensitization to specific aeroallergens
Most epidemiologic studies reporting prevalence of allergic rhinitis (AR) and nonallergic rhinitis (NAR) have assessed solely self-reported prevalence, without confirmation by objective measures. Furthermore, ...
https://ift.tt/2BqTegH
The diagnosis of hereditary angioedema with C1 inhibitor deficiency: a survey of Canadian physicians and laboratories
Hereditary angioedema due to C1 inhibitor deficiency (C1-INH-HAE) is an autosomal dominant disease resulting in random and unpredictable attacks of swelling. The swelling in C1-INH-HAE is a result of impaired ...
https://ift.tt/2KvFDI5
Streptococcus pyogenes bacteremia and toxic shock syndrome related to Strongyloides stercoralis hyperinfection: a case report
We describe a patient with Strongyloides stercoralis hyperinfection associated with Streptococcus pyogenes and with streptococcal toxic shock syndrome. To the best of our knowledge this association has not been p...
https://ift.tt/2DROdQC
Thyroid® High-Impact Articles
FREE ACCESS through December 5, 2018.
Latest Impact Factor: 7.557
The Official Journal of: American Thyroid Association®
Read now:
Mutated Thyroid Hormone Transporter OATP1C1 Associates with Severe Brain Hypometabolism and Juvenile Neurodegeneration
Petter Strømme, Stefan Groeneweg, Elaine C. Lima de Souza, Chantal Zevenbergen, Anette Torgersbråten, Asbjørn Holmgren, Ebrar Gurcan, Marcel E. Meima, Robin P. Peeters, W. Edward Visser, Linda Høneren Johansson, Almira Babovic, Henrik Zetterberg, Heike Heuer, Eirik Frengen, Doriana Misceo, and Theo J. Visser
Metal Coordinated Poly-Zinc-Liothyronine Provides Stable Circulating Triiodothyronine Levels in Hypothyroid Rats
Rodrigo R. Da Conceição, Gustavo W. Fernandes, Tatiana L. Fonseca, Barbara M.L.C. Bocco, and Antonio C. Bianco
Response to Lenvatinib in Children with Papillary Thyroid Carcinoma
Priya Mahajan, Jonathan Dawrant, Albert Kheradpour, Norma M. Quintanilla, Monica E. Lopez, Robert C. Orth, Ioanna Athanassaki, and Rajkumar Venkatramani
Comprehensive Transcriptomic and Genomic Profiling of Subtypes of Follicular Variant of Papillary Thyroid Carcinoma
Young Shin Song, Jae-Kyung Won, Seong-Keun Yoo, Kyeong Cheon Jung, Min Joo Kim, Su-Jin Kim, Sun Wook Cho, Kyu Eun Lee, Ka Hee Yi, Jeong-Sun Seo, and Young Joo Park
Diagnostic Performance of Ultrasound-Based Risk-Stratification Systems for Thyroid Nodules: Comparison of the 2015 American Thyroid Association Guidelines with the 2016 Korean Thyroid Association/Korean Society of Thyroid Radiology and 2017 American Congress of Radiology Guidelines
Eun Ju Ha, Dong Gyu Na, Won-Jin Moon, Young Hen Lee, and Nami Choi
The post <i>Thyroid<sup>®</sup></i> High-Impact Articles appeared first on American Thyroid Association.
https://ift.tt/2OW13P9
Association between atopic dermatitis and extra-cutaneous bacterial and mycobacterial infections: A systematic review and meta-analysis
Atopic dermatitis was found to be associated with increased odds of ear infection, strep throat, urinary tract infection, and multiple other extra-cutaneous infections. Risk of infection should be incorporated into clinical decision-making of atopic dermatitis patients.
https://ift.tt/2DBfWEl
Erratum to “Surgical outcomes in the treatment of temporal bone cerebrospinal fluid leak: A systematic review” [Auris Nasus Larynx 45 (2018) 903–910]
The publisher regrets that the abstract was missing in the original publication of this article.
https://ift.tt/2QaEPxn
Crestal bone loss around dental implants placed in head and neck cancer patients treated with different radiotherapy techniques: a prospective cohort study
The aim of this prospective cohort study was to evaluate how the radiation technique can affect crestal bone loss and the implant survival rate in head and neck cancer patients treated with radiotherapy. In this study, the type of radiotherapy treatment, i.e. three-dimensional conformal radiotherapy (3D-CRT) or intensity modulated radiation therapy (IMRT), was the predictor variable. The primary outcome variable was crestal bone loss, recorded at implant placement and after 3, 6, 12, and 24 months.
https://ift.tt/2PJn2hb
Improving Dermatologic Care for Sexual and Gender Minority Patients Through Routine Data Collection
This Viewpoint explores the routine collection of sexual orientation and gender identity data in dermatology settings to address health disparities faced by sexual and gender minority patients.
https://ift.tt/2KrVLKE
https://ift.tt/2KrVLKE
Pricing a Year of Progression-Free Survival
What is a reasonable price for society to pay to give a patient with cancer 1 extra year of life without tumor growth?
https://ift.tt/2BpJBiu
https://ift.tt/2BpJBiu
Economic Evaluation of Talimogene Laherparepvec Plus Ipilimumab vs Ipilimumab Alone for Unresectable Melanoma
This economic evaluation assesses the cost-effectiveness of talimogene laherparepvec plus ipilimumab combination therapy vs ipilimumab monotherapy in patients with advanced unresectable melanoma from the perspective of public and private US payers.
https://ift.tt/2KqqI1E
https://ift.tt/2KqqI1E
Herpes-Associated Erythema Multiforme
This case report describes the occurrence of herpes-associated erythema multiforme.
https://ift.tt/2Bp3zcZ
https://ift.tt/2Bp3zcZ
Metastatic Calcinosis Cutis Associated With a Selective FGFR Inhibitor
This case report describes metastatic calcinosis cutis associated with selective FGFR inhibitor therapy.
https://ift.tt/2Km5Ybc
https://ift.tt/2Km5Ybc
α-Gal Syndrome vs Chronic Urticaria
This cohort study assesses the use of a blood test to distinguish galactose-α-1,3-galactose (α-gal) allergy from chronic urticaria.
https://ift.tt/2Bq6AtA
https://ift.tt/2Bq6AtA
Use of Dapsone in the Treatment of Chronic Idiopathic and Autoimmune Urticaria
This medical record review evaluates the efficacy and safety of dapsone as second-line therapy in patients with chronic spontaneous urticaria.
https://ift.tt/2KqqF5Y
https://ift.tt/2KqqF5Y
Association of Phenotypic Characteristics and UV Radiation Exposure With Risk of Melanoma
This population-based cohort study uses data from the Norwegian Women and Cancer study to investigate the association between phenotypic characteristics, pattern of UV radiation exposure, and risk of melanoma on different body sites.
https://ift.tt/2BnvuKf
https://ift.tt/2BnvuKf
Association of Hospital Volume With Laryngectomy Outcomes in Patients With Larynx Cancer
This cross-sectional study of patients with a diagnosis of larynx cancer characterizes the hospital volume-outcome association specifically for laryngectomy surgery and identifies a minimum hospital volume threshold associated with improved outcomes.
https://ift.tt/2zfOf0X
https://ift.tt/2zfOf0X
Surgical Volumes and Outcomes
Practice makes perfect. Three words, easily conceptualized, and second nature for any surgeon. It is not surprising that an established and growing body of literature describes improved outcomes when complex surgery is performed by hospitals and surgeons who do those procedures frequently. Luft et al published the foundational study in 1979, reporting on 8 complex operations in almost 1 million patients. For some operations, they found a negative association between the number of procedures performed in a hospital and mortality rates. That general finding has been reproduced using a wide range of data sources for a variety of high-risk operations, including those done for head and neck cancer.
https://ift.tt/2OWroMO
https://ift.tt/2OWroMO
Secondary Endolymphatic Hydrops Associated With Spontaneous Intracranial Hypotension
This case report describes a woman in her 40s who presented with spontaneous intracranial hypotension associated with cerebrospinal fluid leakage.
https://ift.tt/2zfO6dV
https://ift.tt/2zfO6dV
Flat-Panel CT-Guided Place-Pitch Mapping and Speech and Pitch Perception in Cochlear Implant Users
This cohort study evaluates the association between image-based place-pitch mapping using flat-panel computed tomography with speech and pitch perception in individuals with cochlear implants.
https://ift.tt/2OWVhg7
https://ift.tt/2OWVhg7
A Rare Case of a Nasopharyngeal Mass
A man in his 50s with a history of chronic hepatitis C, hypothyroidism, and alcoholism presented to the outpatient clinic complaining of nasal congestion and epistaxis; nonenhanced computed tomography of the paranasal sinuses revealed a soft-tissue mass extending from the nasopharynx into the left sphenoid sinus with erosion of the sphenoid sinus floor and the clivus. What is your diagnosis?
https://ift.tt/2zfNXan
https://ift.tt/2zfNXan
Confluence Model of Sexual Aggression in College Males: Examining Polyvictimization
Violence and Gender, Ahead of Print.
https://ift.tt/2AbccWP
Dysmorphic Short Stature: Radiological Diagnosis of Trichorhinophalangeal Syndrome
Trichorhinophalangeal syndrome (TRPS), a type of skeletal dysplasia, is characterized by a triad of dysmorphic (bulbous nose and large ears); ectodermal (thin and sparse hair); and skeletal (short stature and cone-shaped epiphyses) findings, and this combination is helpful for early diagnosis and appropriate follow-up. A 14-year-old boy presented with short stature and distinctive facial features, and following the first clinical and biological evaluation, no precise diagnosis was reached. Progressive bilateral development of noninflammatory and painless deformity of his second finger required a radiological exam that highlighted the key elements (cone-shaped epiphyses) for final diagnosis. This case illustrates the difficulties to early recognition of TRPS when the clinical presentation is not complete and radiological findings are missing.
https://ift.tt/2DAPvyv
Editorial Board
Publication date: January 2019
Source: Archives of Oral Biology, Volume 97
Author(s):
https://ift.tt/2DEOHJh
Proteomics of the acid-soluble fraction of whole and major gland saliva in burning mouth syndrome patients
Publication date: Available online 20 November 2018
Source: Archives of Oral Biology
Author(s): Tiziana Cabras, Barbara Manconi, Massimo Castagnola, Maria Teresa Sanna, Morena Arba, Shikha Acharya, Jörgen Ekström, Anette Carlén, Irene Messana
Abstract
OBJECTIVE
In the present study the salivary proteome of burning mouth syndrome patients and healthy subjects was characterized by a top-down proteomic approach and compared to highlight possible qualitative and quantitative differences that may give suggestions about the causes of this pathology which are still unknown.
MATERIALS AND METHODS
Resting and stimulated whole saliva, stimulated parotid and submandibular/sublingual saliva samples were collected from burning mouth syndrome patients (n = 16) and age- and gender-matched healthy subjects (n = 14). An equal volume of 0.2% trifluoroacetic acid was added to each sample immediately after collection and the supernatants were analysed by liquid chromatography coupled to electrospray-ionisation mass spectrometry. Proteins and peptides were quantified using a label-free approach measuring the extracted ion current peak areas of the main salivary proteins and peptides.
RESULTS
The quantitation of the main salivary proteins and peptides revealed a higher concentration of cystatin SN in resting saliva of burning mouth syndrome patients with respect to healthy controls and no other conspicuous changes.
CONCLUSIONS
The reported data showed that the salivary protein profile was not affected, in composition and relative abundance, by the burning mouth syndrome, except for the cystatin SN, a protein up-regulated in several pathological conditions, that might be considered potentially indicative of the disease.
https://ift.tt/2Qdxayq
Peptides from rice endosperm protein restrain periodontal bone loss in mouse model of periodontitis
Publication date: Available online 20 November 2018
Source: Archives of Oral Biology
Author(s): Hikaru Tamura, Tomoki Maekawa, Hisanori Domon, Takumi Hiyoshi, Daisuke Yonezawa, Kosuke Nagai, Akihito Ochiai, Masayuki Taniguchi, Koichi Tabeta, Takeyasu Maeda, Yutaka Terao
Abstract
Objective
Food-derived peptides have been reported to exhibit antibacterial activity against periodontal pathogenic bacteria. However, no effect has been shown on inflammation and bone resorption in periodontal pathology. The overall objective of the current study was to investigate how rice peptides influence biological defense mechanisms against periodontitis-induced inflammatory bone loss, and identify their novel functions as a potential anti-inflammatory drug.
Design
The expression of inflammatory and osteoclast-related molecules was examined in mouse macrophage-derived RAW 264.7 cell cultures using qPCR. Subsequently, the effect of these peptides on inflammatory bone loss in mouse periodontitis was examined using a mouse model of tooth ligation. Briefly, periodontal bone loss was induced for 7 days in mice by ligating the maxillary second molar and leaving the contralateral tooth un-ligated (baseline control). The mice were microinjected daily with the peptide in the gingiva until the day before euthanization. One week after the ligation, TRAP-positive multinucleated cells (MNCs) were enumerated from five random coronal sections of the ligated sites in each mouse.
Results
Rice peptides REP9 and REP11 significantly inhibited transcription activity of inflammatory and osteoclast-related molecules. Local treatment with the rice peptides, in mice subjected to ligature-induced periodontitis, inhibited inflammatory bone loss, explaining the decreased numbers of osteoclasts in bone tissue sections.
Conclusion
Therefore, these data suggested that the rice peptides possess a protective effect against periodontitis.
https://ift.tt/2DCgOJ2
Leptin Stimulates DMP-1 and DSPP Expression in Human Dental Pulp via MAPK 1/3 and PI3K Signaling Pathways
Publication date: Available online 20 November 2018
Source: Archives of Oral Biology
Author(s): Jenifer Martín-González, Antonio Pérez-Pérez, Daniel Cabanillas-Balsera, Teresa Vilariño-García, Victor Sánchez-Margalet, Juan José Segura-Egea
Abstract
Introduction
: To investigate the physiological function of leptin in human dental pulp, and to determine the specific pathways implicated in its effect.
Methods
Twenty-seven dental pulp samples were obtained from human third molars. Pulp samples were treated with or without human recombinant leptin. Leptin functional effect was analyzed in terms of regulation of the synthesis levels of DSPP and DMP-1, determined by immunoblot.
Results
Leptin stimulated DMP-1 and DSPP synthesis in all human dental pulp specimens. The stimulatory effect of leptin on DMP-1 and DSPP synthesis was partially prevented by blocking mitogen-activated protein kinase (MAPK 1/3) and phosphatidylinositol 3 kinase (PI3K) pathways, respectively.
Conclusions
The present study demonstrates the functional effect of leptin in human dental pulp stimulating the expression of DMP-1 and DSPP, both proteins implicated in dentinogenesis. Leptin stimulates DSPP expression via PI3K pathway and DMP-1 synthesis via MAPK 1/3 pathway. These results support the role of leptin in pulpal reparative response, opening a new research line that could have translational application to the clinic in vital pulp therapy procedures.
https://ift.tt/2Qapug9
Betamethasone suppresses the inflammatory response in LPS-stimulated dental pulp cells through inhibition of NF-κB
Publication date: Available online 20 November 2018
Source: Archives of Oral Biology
Author(s): Dan Wang, Ning-Xin Zhu, Man Qin, Yuan-Yuan Wang
Abstract
Objective
This study aimed to investigate the anti-inflammatory effect of betamethasone on LPS-stimulated human dental pulp stem cells (DPSCs) and its associated mechanism. The osteo-/odontogenic differentiation and osteoclast effect of betamethasone on DPSCs and stem cells from human exfoliated deciduous teeth (SHED) were evaluated.
Design
The proliferative effect of betamethasone on DPSCs was analyzed using a cholecystokinin octapeptide assay. The anti-inflammatory effect of betamethasone was investigated using quantitative polymerase chain reaction (qPCR) and ELISA. The anti-inflammatory mechanism was explored using qPCR, Western blot, and immunofluorescence staining. Glucocorticoid receptor antagonist mifepristone was applied to verify the anti-inflammatory mechanism of betamethasone. The osteo-/odontogenic differentiation and osteoclast effect of betamethasone on DPSCs and SHED were detected by qPCR.
Results
1 μg L-1 betamethasone was found to have the strongest effect on DPSCs proliferation. The expression of pro-inflammatory cytokines and mediators, as well as prostaglandin E2 (PGE2) were significantly decreased following treatment with betamethasone in LPS- stimulated DPSCs. They were also decreased in response to an NF-κB inhibitor, Bay 11-7082. Betamethasone and Bay 11-7082 significantly inhibited the expression of p-p65 and promoted the nuclear exclusion of p65. The anti-inflammatory effect of betamethasone was clearly reversed by adding mifepristone. Gene expression associated with osteo-/odontogenic differentiation was significantly up-regulated in betamethasone and osteogenic media (OM) treated groups. The ratio of the receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) at the mRNA level was suppressed in DPSCs and elevated in SHED.
Conclusions
Betamethasone has an anti-inflammatory effect on LPS- stimulated DPSCs through a blockade of NF-κB activation and exhibits an osteo-/odonto-inductive effect on DPSCs and SHED. Although betamethasone displays an osteoclast effect on SHED.
https://ift.tt/2DAKR3x
Editorial Board
Publication date: January 2019
Source: Archives of Oral Biology, Volume 97
Author(s):
https://ift.tt/2DEOHJh
Proteomics of the acid-soluble fraction of whole and major gland saliva in burning mouth syndrome patients
Publication date: Available online 20 November 2018
Source: Archives of Oral Biology
Author(s): Tiziana Cabras, Barbara Manconi, Massimo Castagnola, Maria Teresa Sanna, Morena Arba, Shikha Acharya, Jörgen Ekström, Anette Carlén, Irene Messana
Abstract
OBJECTIVE
In the present study the salivary proteome of burning mouth syndrome patients and healthy subjects was characterized by a top-down proteomic approach and compared to highlight possible qualitative and quantitative differences that may give suggestions about the causes of this pathology which are still unknown.
MATERIALS AND METHODS
Resting and stimulated whole saliva, stimulated parotid and submandibular/sublingual saliva samples were collected from burning mouth syndrome patients (n = 16) and age- and gender-matched healthy subjects (n = 14). An equal volume of 0.2% trifluoroacetic acid was added to each sample immediately after collection and the supernatants were analysed by liquid chromatography coupled to electrospray-ionisation mass spectrometry. Proteins and peptides were quantified using a label-free approach measuring the extracted ion current peak areas of the main salivary proteins and peptides.
RESULTS
The quantitation of the main salivary proteins and peptides revealed a higher concentration of cystatin SN in resting saliva of burning mouth syndrome patients with respect to healthy controls and no other conspicuous changes.
CONCLUSIONS
The reported data showed that the salivary protein profile was not affected, in composition and relative abundance, by the burning mouth syndrome, except for the cystatin SN, a protein up-regulated in several pathological conditions, that might be considered potentially indicative of the disease.
https://ift.tt/2Qdxayq
Peptides from rice endosperm protein restrain periodontal bone loss in mouse model of periodontitis
Publication date: Available online 20 November 2018
Source: Archives of Oral Biology
Author(s): Hikaru Tamura, Tomoki Maekawa, Hisanori Domon, Takumi Hiyoshi, Daisuke Yonezawa, Kosuke Nagai, Akihito Ochiai, Masayuki Taniguchi, Koichi Tabeta, Takeyasu Maeda, Yutaka Terao
Abstract
Objective
Food-derived peptides have been reported to exhibit antibacterial activity against periodontal pathogenic bacteria. However, no effect has been shown on inflammation and bone resorption in periodontal pathology. The overall objective of the current study was to investigate how rice peptides influence biological defense mechanisms against periodontitis-induced inflammatory bone loss, and identify their novel functions as a potential anti-inflammatory drug.
Design
The expression of inflammatory and osteoclast-related molecules was examined in mouse macrophage-derived RAW 264.7 cell cultures using qPCR. Subsequently, the effect of these peptides on inflammatory bone loss in mouse periodontitis was examined using a mouse model of tooth ligation. Briefly, periodontal bone loss was induced for 7 days in mice by ligating the maxillary second molar and leaving the contralateral tooth un-ligated (baseline control). The mice were microinjected daily with the peptide in the gingiva until the day before euthanization. One week after the ligation, TRAP-positive multinucleated cells (MNCs) were enumerated from five random coronal sections of the ligated sites in each mouse.
Results
Rice peptides REP9 and REP11 significantly inhibited transcription activity of inflammatory and osteoclast-related molecules. Local treatment with the rice peptides, in mice subjected to ligature-induced periodontitis, inhibited inflammatory bone loss, explaining the decreased numbers of osteoclasts in bone tissue sections.
Conclusion
Therefore, these data suggested that the rice peptides possess a protective effect against periodontitis.
https://ift.tt/2DCgOJ2
Leptin Stimulates DMP-1 and DSPP Expression in Human Dental Pulp via MAPK 1/3 and PI3K Signaling Pathways
Publication date: Available online 20 November 2018
Source: Archives of Oral Biology
Author(s): Jenifer Martín-González, Antonio Pérez-Pérez, Daniel Cabanillas-Balsera, Teresa Vilariño-García, Victor Sánchez-Margalet, Juan José Segura-Egea
Abstract
Introduction
: To investigate the physiological function of leptin in human dental pulp, and to determine the specific pathways implicated in its effect.
Methods
Twenty-seven dental pulp samples were obtained from human third molars. Pulp samples were treated with or without human recombinant leptin. Leptin functional effect was analyzed in terms of regulation of the synthesis levels of DSPP and DMP-1, determined by immunoblot.
Results
Leptin stimulated DMP-1 and DSPP synthesis in all human dental pulp specimens. The stimulatory effect of leptin on DMP-1 and DSPP synthesis was partially prevented by blocking mitogen-activated protein kinase (MAPK 1/3) and phosphatidylinositol 3 kinase (PI3K) pathways, respectively.
Conclusions
The present study demonstrates the functional effect of leptin in human dental pulp stimulating the expression of DMP-1 and DSPP, both proteins implicated in dentinogenesis. Leptin stimulates DSPP expression via PI3K pathway and DMP-1 synthesis via MAPK 1/3 pathway. These results support the role of leptin in pulpal reparative response, opening a new research line that could have translational application to the clinic in vital pulp therapy procedures.
https://ift.tt/2Qapug9
Betamethasone suppresses the inflammatory response in LPS-stimulated dental pulp cells through inhibition of NF-κB
Publication date: Available online 20 November 2018
Source: Archives of Oral Biology
Author(s): Dan Wang, Ning-Xin Zhu, Man Qin, Yuan-Yuan Wang
Abstract
Objective
This study aimed to investigate the anti-inflammatory effect of betamethasone on LPS-stimulated human dental pulp stem cells (DPSCs) and its associated mechanism. The osteo-/odontogenic differentiation and osteoclast effect of betamethasone on DPSCs and stem cells from human exfoliated deciduous teeth (SHED) were evaluated.
Design
The proliferative effect of betamethasone on DPSCs was analyzed using a cholecystokinin octapeptide assay. The anti-inflammatory effect of betamethasone was investigated using quantitative polymerase chain reaction (qPCR) and ELISA. The anti-inflammatory mechanism was explored using qPCR, Western blot, and immunofluorescence staining. Glucocorticoid receptor antagonist mifepristone was applied to verify the anti-inflammatory mechanism of betamethasone. The osteo-/odontogenic differentiation and osteoclast effect of betamethasone on DPSCs and SHED were detected by qPCR.
Results
1 μg L-1 betamethasone was found to have the strongest effect on DPSCs proliferation. The expression of pro-inflammatory cytokines and mediators, as well as prostaglandin E2 (PGE2) were significantly decreased following treatment with betamethasone in LPS- stimulated DPSCs. They were also decreased in response to an NF-κB inhibitor, Bay 11-7082. Betamethasone and Bay 11-7082 significantly inhibited the expression of p-p65 and promoted the nuclear exclusion of p65. The anti-inflammatory effect of betamethasone was clearly reversed by adding mifepristone. Gene expression associated with osteo-/odontogenic differentiation was significantly up-regulated in betamethasone and osteogenic media (OM) treated groups. The ratio of the receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) at the mRNA level was suppressed in DPSCs and elevated in SHED.
Conclusions
Betamethasone has an anti-inflammatory effect on LPS- stimulated DPSCs through a blockade of NF-κB activation and exhibits an osteo-/odonto-inductive effect on DPSCs and SHED. Although betamethasone displays an osteoclast effect on SHED.
https://ift.tt/2DAKR3x
Editorial Board
Publication date: November 2018
Source: British Journal of Oral and Maxillofacial Surgery, Volume 56, Issue 9
Author(s):
https://ift.tt/2OSdqM0
Training Groups
Publication date: November 2018
Source: British Journal of Oral and Maxillofacial Surgery, Volume 56, Issue 9
Author(s):
https://ift.tt/2zmq6Wy
Special section on intensity-modulated radiation therapy for head and neck cancer (IMRT)
Publication date: January 2019
Source: Oral Oncology, Volume 88
Author(s): Vincent Grégoire, Wai Tong Ng
https://ift.tt/2R07emV
The impact of treatment package time on survival in surgically managed head and neck cancer in the United States
Publication date: January 2019
Source: Oral Oncology, Volume 88
Author(s): Alexander N. Goel, Mariana I. Frangos, Govind Raghavan, Stephanie L. Lazaro, Belicia Tang, Dinesh K. Chhetri, Jennifer L. Long, Maie A. St. John
Abstract
Objectives
Delays in the initiation of postoperative radiation have been associated with worse outcomes; however, the effect of the overall treatment package time (interval from surgery through the completion of radiation) remains undefined. The purpose of this study was to determine the impact of package time on survival and to evaluate this effect among different subgroups of head and neck cancer patients.
Patients and methods
In this observational cohort study, the National Cancer Database was used to identify 35,167 patients with resected nonmetastatic head and neck cancer who underwent adjuvant radiation from 2004 to 2014. Kaplan-Meier survival estimates and multivariate Cox regression analyses were performed to determine the effect of treatment package time on overall survival.
Results
Median package time was 96 days (interquartile range, 85–112 days). After adjusting for covariates, package times of 11 weeks or less were associated with improved survival (adjusted hazard ratio (aHR), 0.90; 95% confidence interval, 0.83–0.97) compared to an interval of 12–13 weeks, whereas package times of more than 14 weeks were associated with worse survival (aHR, 1.14, 1.14, and 1.22 for 14–15, 15–17, and >17 weeks, respectively). A significant interaction was identified between package time and disease site, nodal status, and stage. Specifically, patients with oropharyngeal tumors, advanced stage (III or IV) disease, or nodal involvement experienced more pronounced increases in mortality risk with delays in treatment time.
Conclusions
Treatment package time independently impacts survival. This effect may be strongest for patients with oropharyngeal tumors or advanced stage disease.
https://ift.tt/2PIgyzq
Macrophages in the microenvironment of head and neck cancer: potential targets for cancer therapy
Publication date: January 2019
Source: Oral Oncology, Volume 88
Author(s): Diane Evrard, Petr Szturz, Annemilaï Tijeras-Raballand, Lucile Astorgues-Xerri, Chloé Abitbol, Valérie Paradis, Eric Raymond, Sébastien Albert, Béatrix Barry, Sandrine Faivre
Abstract
The microenvironment of solid tumors has become a promising target for future therapies modulating immune cells. Patients with advanced head and neck cancer, which still portends a poor outcome, are particularly in need of innovative approaches. In oral squamous cell carcinoma, high density of tumor-associated macrophages (TAMs) appears consistently associated with poor prognosis, whereas data are currently limited for other head and neck sites. Several approaches to block TAMs have been investigated, including TAMs inactivation by means of the colony stimulating factor 1 (CSF-1)/CSF-1 receptor (CSF-1R) inhibitors or strategies to reprogram TAMs from M2 protumoral phenotype toward M1 antitumoral phenotype. This review focuses on both prognostic and therapeutic aspects related to TAMs in head and neck carcinomas.
Graphical abstract
https://ift.tt/2R0lLPH
Surgical deroofing in the treatment of patients with auricular pseudocyst
Publication date: Available online 20 November 2018
Source: Auris Nasus Larynx
Author(s): Yoon Jae Lee, Jin Geun Kwon, Hyun Ho Han
Abstract
Objective
An auricular pseudocyst is a fluid filled cavity, characterized by a lack of epithelium, in the intra-cartilaginous space. Clinically, it presents as a painless lump on the upper anterior surface of the ear. Various treatment methods have been discussed in the literature, including aspiration, incision and drainage, or steroid injection. However, these approaches are associated with a high rate of recurrence and results are often esthetically unsatisfactory; therefore, a need for improved treatment approaches remains.
Methods
From March, 2015, to June, 2017, 15 patients with auricular pseudocyst were treated with surgical deroofing followed by local contour pressure dressing with a simple bolster. In addition, the structure of the auricular pseudocyst was assessed at a microscopic level.
Results
Patients were followed up for a mean period of 12 months, during which time there were no reports of postoperative complications or recurrence. The results were cosmetically excellent in all patients and no cartilage deformity was seen. Considering the composition of pseudocysts seen on microscopic evaluation, deroofing to remove the anterior leaflet and removal of debris from the posterior leaflet is an appropriate treatment modality.
Conclusion
This reliable and simple approach may be recommended as first line treatment for auricular pseudocysts to avoid recurrence and complications associated with other treatment modalities, such as cartilage deformity, skin depigmentation, and scarring.
https://ift.tt/2PJxHIX
Mast cells as protectors of health
Publication date: Available online 20 November 2018
Source: Journal of Allergy and Clinical Immunology
Author(s): Anne Dudeck, Martin Köberle, Oliver Goldmann, Nicole Meyer, Jan Dudeck, Stefanie Lemmens, Manfred Rohde, Nestor González Roldán, Kirsten Dietze-Schwonberg, Zane Orinska, Eva Medina, Sven Hendrix, Martin Metz, Ana Claudia Zenclussen, Esther von Stebut, Tilo Biedermann
Abstract
Mast cells (MC), well known for their effector functions in Th2 skewed allergic and also autoimmune inflammation, become increasingly acknowledged for their role in protection of health. It is now clear that they are also key modulators of immune responses at interface organs like skin or gut. MC can prime tissues for adequate inflammatory responses and cooperate with dendritic cells in T cell activation. They also regulate harmful immune responses in trauma and help to successfully orchestrate pregnancy. This review focusses on the beneficial effects of mast cells on tissue homeostasis and elimination of toxins or venoms. MC can enhance pathogen clearance in many bacterial, viral, and parasite infections, e.g. by TLR2 triggered degranulation, secretion of antimicrobial cathelicidins, recruiting neutrophils or by providing extracellular DNA traps. The role of MC in tumors is more ambiguous, however, encouraging new findings show they can change the tumor microenvironment towards anti-tumor immunity when adequately triggered. Uterine tissue remodeling by α-chymase (MCP-5) is crucial for successful embryo implantation. MCP-4 and the tryptase MCP-6 emerge to be protective in CNS trauma by reducing inflammatory damage and excessive scar formation, thereby protecting axon growth. Last but not least, we see proteases like carboxypeptidase A released by FcεRI activated MC detoxify an increasing number of venoms and endogenous toxins. A better understanding of the plasticity of MC will help to improve these advantageous effects, and hint on ways to cut down detrimental MC actions.
https://ift.tt/2Acmbv5
Inter-laboratory variability in multiplexed pneumococcal antibody testing
Publication date: Available online 20 November 2018
Source: Journal of Allergy and Clinical Immunology
Author(s): David C. LaFon, Moon H. Nahm
Summary
This pragmatic, updated assessment of variability among pneumococcal antibody assays suggests that variability may now be greater than previously reported, and potentially influential in clinical decision making.
https://ift.tt/2Tx7Xxy
Impact of Ixekizumab Treatment on Itch and Psoriasis Area and Severity Index in Patients with Moderate-to-Severe Plaque Psoriasis: An Integrated Analysis of Two Phase III Randomized Studies
Abstract
Introduction
We evaluated baseline itch and its impact on the efficacy of ixekizumab (IXE) in clearing psoriasis and improving quality-of-life measures, and we explored the relationship between itch and psoriatic skin improvement.
Methods
Data were analyzed from two double-blind, randomized, controlled phase III studies (UNCOVER-2/3) comparing etanercept (ETN), IXE, and placebo (PBO) in patients with moderate-to-severe plaque psoriasis. Long-term analysis included UNCOVER-3 data from week 0 to week 156.
Results
At week 12, a clinically meaningful improvement in itch [Itch Numeric Rating Scale (NRS) reduction ≥ 4] was seen in 70.0%, 88.6%, and 90.8% of the IXE-treated patients in the baseline Itch NRS 4–6, 7–8, and 9–10 groups, respectively (all itch severity groups p < 0.001 versus ETN and PBO). Also, 68.9%, 67.1%, and 73.6% of the IXE-treated patients in the baseline Itch NRS 4–6, 7–8, and 9–10 groups, respectively, showed an improvement of ≥ 90.0% in the Psoriatic Area and Severity Index (PASI) at week 12 as compared to the baseline (PASI 90) (all itch severity groups p < 0.001 versus ETN and PBO). For most patients, itch reduction preceded psoriatic plaque improvement. Sustained effects of IXE on itch and PASI were observed during 3 years of treatment.
Conclusions
Regardless of baseline itch severity, IXE treatment provided a rapid improvement in itch followed by clinically meaningful improvements in psoriasis.
Funding
Eli Lilly and Company.
Trial registration
ClinicalTrials.gov identifiers, NCT01597245 and NCT01646177.
https://ift.tt/2zlyVQd
An alphavirus-based therapeutic cancer vaccine: from design to clinical trial
Abstract
Cancer immunotherapy has greatly advanced in recent years. Most immunotherapeutic strategies are based on the use of immune checkpoint blockade to unleash antitumor immune responses or on the induction or adoptive transfer of immune effector cells. We aim to develop therapeutic vaccines based on recombinant Semliki Forest virus vectors to induce tumor-specific effector immune cells. In this review, we describe our ongoing work on SFV-based vaccines targeted against human papillomavirus- and hepatitis C virus-related infections and malignancies, focusing on design, delivery, combination strategies, preclinical efficacy and product development for a first-in-man clinical trial with an HPV-specific vaccine.
https://ift.tt/2DNO5BV
A Rare Cause of Acute Hepatopancreatitis in a Nepalese Teen
Background. Acute pancreatitis (AP) coexisting with acute hepatitis (AH) in children is uncommon. Moreover, a single bacterial cause explaining both the complications is even rarer. Despite familiarity with the usual presentation of enteric fever, atypical presentations can go unnoticed. Case Presentation. A 16-year-old previously healthy male presented to the emergency unit with recurrent swinging pyrexia, abdominal symptoms, and jaundice for a week. Blood work-up revealed deranged liver function tests (LFTs) and elevated pancreatic enzymes. Further assessment with imaging made a diagnosis of AH and AP without necrosis. Blood culture positivity for Salmonella typhi eventually confirmed the diagnosis. Conclusion. This is an uncommon presentation of an infection that is fairly common in our part of globe. Nevertheless, early suspicion and recognition is the key to timely management. Regular follow-ups are required to pick complications up early.
https://ift.tt/2Q9WgxX
Correlates and Outcomes of Low Physical Activity Posttransplant: A Systematic Review and Meta-Analysis
Background Little is known about associations between low physical activity (PA) and its correlates and outcomes in solid organ transplant recipients. This systematic review with meta-analysis examined correlates and outcomes associated with low PA (i.e. not meeting individual study's definition of being physically active) following solid organ transplantation. Methods We searched PubMed, CINAHL, PsycINFO and EMBASE from inception to February 2016 to identify peer-reviewed data-based articles. Articles published in English, German, Spanish, French, Italian, Portuguese, or Dutch that examined correlates or outcomes associated with low PA in adult single, solid organ transplant recipients were included. Studies' quality was assessed using a 14-item checklist. Pooled odds ratios and 95% confidence intervals were computed for correlates and outcomes examined in ≥ 5 studies. Results Of 7,401 publications screened, 34 studies met inclusion criteria and were included in the overall synthesis with 15 included in the meta-analysis. Most focused on renal transplantation (n=18, 53%) and used cross-sectional designs (n=26, 77%). Of 30 correlates examined, [condition-related (n=11), social/economic-related (n=9), patient-related (n=4), healthcare system-related (n=3), and treatment-related (n=3)], only four were examined ≥5 times and included in meta-analyses. None were significantly related to low PA. Of 19 outcomes assessed, only physical health-related quality of life was examined ≥ 5 times. Low PA was significantly associated with low physical health-related quality of life (OR=0.172, 95% CI=0.08, 0.37). Conclusions We found few studies examining most correlates and outcomes related to low PA despite growing evidence that improving PA might be an effective intervention in improving post-transplant outcomes. Address for Correspondence: Sabina De Geest, Institute of Nursing Science, Department of Public Health, University of Basel, Bernoullistrasse 28, Basel, Switzerland, Tel +41 61 2070951, sabina.degeest@unibas.ch Protocol registration: PROSPERO protocol CRD42015003333 Authership contributions: Lut Berben: Participated in research design, performance of the research (design of search strategy, title and abstract screening, full text evaluation, data extraction), data analysis (effect size calculation), and wrote the paper. Sandra J. Engberg: Participated in research design, performance of the research (design of search strategy, full text evaluation, data extraction), data analysis (effect size calculation), participating in writing the paper. Anja Rossmeissl: Participated in research design, performance of the research (design of search strategy, title and abstract screening, full text evaluation, data extraction), and participating in writing the paper. Elisa J. Gordon: Participated in research design, performance of the research (design of search strategy, title and abstract screening, full text evaluation, data extraction), and participating in writing the paper. Fabienne Dobbels: Participated in research design, performance of the research (design of search strategy, title and abstract screening, full text evaluation, data extraction), data analysis (effect size calculation), and critical review of the paper. Christiane Kugler: Participated in research design, performance of the research (design of search strategy, title and abstract screening, full text evaluation, data extraction), data analysis (effect size calculation), and critical review of the paper. Arno Schmidt-Trucksäss: Participated in research design, performance of the research (design of search strategy, title and abstract screening, full text evaluation, data extraction), data analysis (effect size calculation), and critical review of the paper. Mary Lou Klem: Design of search strategy, programming of searches in different databases, feedback on methodology used to conduct the systematic review; critical revision of the paper. Susan M. Sereika: Design of the Access databases for data input, advice on methodology to be used for effect size calculation, review of statistical analyses, and critical revision of the paper. Paolo De Simone: Participated in the design of the study, overall project management and quality control of methodology followed, and critical revision of the paper. Fabienne Dobbels: Participated in the design of the study, overall project management and quality control of methodology followed, and critical revision of the paper. Sabina M. De Geest: Participated in the design of the study, overall project management and quality control of methodology followed, and critical revision of the paper. Disclosure No author states any conflicts of interest. Funding The research has received funding for a consortium meeting from the Brocher Foundation, a Swiss non-profit private foundation. Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.
https://ift.tt/2PFyz12
https://ift.tt/2PFyz12
Enrichment of cytomegalovirus-induced NKG2C+ Natural Killer cells in the lung allograft
Background In lung transplant recipients, immunosuppressive medications result in impaired antiviral immunity and a propensity for cytomegalovirus (CMV) reactivation within the lung allograft. Natural Killer (NK) cells play a key role in immunity to CMV, with an increase in the proportion of NK cells expressing activating CD94-NKG2C receptors in the blood being a strong correlate of CMV infection. Whether a similar increase in NKG2C+ NK cells occurs in lung transplant recipients following CMV reactivation in the allograft and if such cells contribute to viral control, remains unclear. Methods In this pilot study, we longitudinally assessed the frequency and phenotype of NKG2C+ NK cells in the blood and bronchoalveolar lavage (BAL) of lung transplant recipients and stratified recipients based on their risk of developing CMV disease. Results We observed an increase in the proportion of NKG2C+ NK cells in the blood and BAL of CMV high-risk patients, coincident with both the cessation of anti-viral prophylaxis and subsequent detection of actively replicating CMV in the blood and/or lung allograft. Additionally, these NKG2C+ NK cells expressed killer-cell immunoglobulin-like receptors (KIR) distinct from those of other NK subsets and BAL NKG2C+ NK cells possessed an activated phenotype. Finally, the frequency of NKG2C+ NK cells in the BAL may be inversely correlated with CMV blood titers. Conclusions Monitoring the phenotype of NK cells post-lung transplant may be a useful biomarker for monitoring patient levels of CMV immunity. # Joint senior authors * Corresponding author: Lucy C. Sullivan, Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia Email: lcsull@unimelb.edu.au Authorship: Conceived and designed experiments: L.C.S., A.J.B., G.P.W. Performed the experiments: C.M.H., J.M.L.W., A.K., L.C.S. Analyzed the data: C.M.H, L.C.S., S.S. Contributed reagents/materials/analysis tools: Y.C., B.L., G.I.S., G.P.W. Wrote the paper: L.C.S., C.M.H., S.S., G.I.S., G.P.W. Disclosures: The authors declare no conflicts of interest Funding: National Health and Medical Research Council (NHMRC) Program Grant (A.G.W.), NHMRC Project Grant (L.C.S. and G.P.W.). We acknowledge the Margaret Pratt Foundation and the Lungitude Foundation for ongoing support. Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.
https://ift.tt/2QZVl0p
https://ift.tt/2QZVl0p
Report of the 24th Annual Congress of the International Liver Transplantation Society
The 24th Joint Annual Congress of the International Liver Transplantation Society (ILTS) in association with European Liver and Intestine Transplant Association (ELITA) and Liver Intensive Care Group of Europe (LICAGE) was held in Lisbon, Portugal from May 23-26, 2018. More than 1200 participants from over 60 countries including surgeons, hepatologists, anesthesiologists and critical care intensivists, radiologists, pathologists, organ procurement personnel and research scientists, came together with the common aim of improving care and outcomes for liver transplant recipients. Over 600 scientific abstracts were presented. The principal themes were living donation, use of marginal liver donors, machine preservation, disease-specific immunosuppressive regimen, malignancies, and advances in pediatric liver transplantation and liver transplant anaesthesia. This report presents excerpts from invited lectures, and select abstracts from scientific sessions, which add to current knowledge, and will drive clinical practice and future research. * Eleonora De Martin and Amelia Hessheimer are co-first authors Corresponding Author and Reprint Requests: Prashant Bhangui, Medanta Institute of Liver Transplantation and Regenerative Medicine, Medanta-The Medicity, Sector 38, Gurugram, Delhi NCR, India. Email: pbhangui@gmail.com AUTHORSHIP PAGE Eleonora De Martin - Participated in study design, data collection, writing of the paper Amelia Hessheimer - Participated in study design, data collection, writing of the paper Ryan Chadha - Participated in study design, data collection, writing of the paper Gokhan Kabacam - Participated in study design, data collection, writing of the paper Jeremy Raganayagam - Participated in study design, data collection, writing of the paper Varvara Kirchner - Participated in study design, data collection Marit Kalisvaart - Participated in study design, data collection Irene Scalera - Participated in study design, data collection Mamatha Bhat - Participated in study design, data collection Alan Contreras - Participated in study design, data collection Prashant Bhangui - Participated in study design, writing of the paper, critical appraisal Disclosure: The authors declare no conflicts of interest. Funding or Grants: None to declare Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.
https://ift.tt/2R0htYi
https://ift.tt/2R0htYi
Young people with biliary atresia requiring liver transplantation: a distinct population requiring specialist care
Background Young people born with biliary atresia are an emerging population for adult hepatologists with 40-45% of children entering adolescence with their native liver intact. For those requiring liver transplantation during adolescence, disparity on the waiting list and post liver transplantation outcome for young adults compared to younger and older age groups has stimulated discussion regarding the optimal timing of listing. In this study we review our experience of young people with biliary atresia requiring liver transplantation during adolescence and young adulthood. Methods Retrospective, single centre review of patients with biliary atresia requiring liver transplantation > 11 years. Results Thirty-six young people (16 male) underwent liver transplantation between 1991-2014 at a median age of 16.6 (IQR 14.2,19.5) years. The commonest indications for listing were refractory cholangitis (31%), synthetic failure (25%) and variceal bleeding (14%). Patients listed by the adult team (n=14) waited longer than those listed by the paediatric team (10 (IQR 7.7,24.6) vs 5.8 (IQR 4.0,15.1) months (p
https://ift.tt/2PFys5C
https://ift.tt/2PFys5C
Human Atrial Natriuretic Peptide in Cold Storage of Donation after Circulatory Death Rat Livers: An Old but New Agent for Protecting Vascular Endothelia?
Background Current critical shortage of donor organs has increased the use of donation after circulatory death (DCD) livers for transplantation, despite higher risk for primary non-function or ischemic cholangiopathy. Human atrial natriuretic peptide (hANP) is a cardiovascular hormone that possesses protective action to vascular endothelia. We aimed to clarify the therapeutic potential of hANP in cold storage of DCD livers. Methods Male Wistar rats were exposed to 30-minute warm ischemia in situ. Livers were then retrieved and cold-preserved for 6 hours with or without hANP supplementation. Functional and morphological integrity of the livers was evaluated by oxygenated ex vivo reperfusion at 37°C. Results hANP supplementation resulted in significant reduction of portal venous pressure (12.2 ± 0.5 vs. 22.5 ± 3.5 mmHg, P
https://ift.tt/2R1B4Y8
https://ift.tt/2R1B4Y8
The effects of oxygenation on ex vivo kidneys undergoing Hypothermic Machine Perfusion
Background Supplemental oxygenation of the standard Hypothermic Machine Perfusion (HMP) circuit has the potential to invoke favourable changes in metabolism, optimising cadaveric organs prior to transplantation. Methods Eight pairs of porcine kidneys underwent 18 hours of either oxygenated (HMP/O2) or aerated (HMP/Air) HMP in a paired DCD model of transplantation. Circulating perfusion fluid was supplemented with the metabolic tracer universally labelled glucose ([U-13C] glucose). Perfusate, end-point renal cortex and medulla samples underwent metabolomic analysis using 1D and 2D NMR experiments in addition to GC-MS. Analysis of 13C labelled metabolic products was combined with adenosine nucleotide levels and differences in tissue architecture. Results Metabolomic analysis revealed significantly higher concentrations of universally labelled lactate in the cortex of HMP/Air vs HMP/O2 kidneys (0.056 mM vs. 0.026 mM, p
https://ift.tt/2PFyjPC
https://ift.tt/2PFyjPC
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