Markers to sensibility and relapse on IMR-32 neuroblastoma cell line cultured in monolayer (2D) and neurosphere (3D) models cisplatin-treated

xlomafota13 shared this article with you from Inoreader Markers to sensibility and relapse on IMR-32 neuroblastoma cell line cultured in monolayer (2D) and neurosphere (3D) models cisplatin-treated Via histochem Acta Histochem. 2022 Jan 13;124(2):151849. doi: 10.1016/j.acthis.2022.151849. Online ahead of print. ABSTRACT The complexity of different components of tumor stroma poses huge challenges for therapies targeting the neuroblastoma (NB) microenvironment. The present study aimed to evaluate platinum-based response in IMR-32 neuroblastoma cell line cultured in monolayer (2D) and neurosphere (3D) models. For this, we evaluated mRNA expression of heat shock proteins HSPA1A, HSPB1, TRAP1, HSPA1AL, HSPD1, and DNA damage repair gene ERCC1. After treatment, residual cells were grafted on CAM (chicken chorioallantoic membrane) to evaluate the growth capability and histological paraffin sections were made to assess Ki-67 and HER-2 proteins by immunofluorescence. Our results showed that cisplatin induces mRNA downregulation of Heat Shock Proteins and ERCC1 in IMR-32 cells cultured in 2D or 3D models. In addition, the cisplatin-treatment approach increased HER-2 expression in residual IMR-32 cells grafted on the CAM. Therefore, these insights provide many advances in neuroendocrine tumor biology and knowledge about cisplatin-response in neuroblastoma. PMID:35033934 | DOI:10.1016/j.acthis.2022.151849 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Prosthetic voice rehabilitation after laryngoesophagectomy: surgical and functional outcomes

xlomafota13 shared this article with you from Inoreader Prosthetic voice rehabilitation after laryngoesophagectomy: surgical and functional outcomes Via http://link.springer.com/journal/405,European archives of oto-rhino-laryngology,The European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino Eur Arch Otorhinolaryngol. 2022 Jan 16. doi: 10.1007/s00405-022-07251-8. Online ahead of print. ABSTRACT PURPOSE: Quality of Life (QoL) after extensive head and neck resections is of paramount importance, especially after pharyngolaryngectomy or pharyngolaryngoesophagectomy where complex digestive tract reconstruction is required (with gastric pull-up or colon transposition). Tracheodigestive puncture (TDP) is the only vocal restoration option in this group of patients. The aim of this study is to evaluate postoperative complications, vocal outcomes, voice-related and swallowing-related QoL after secondary TDP in this cohort of patients. METHODS: A retrospective study was conducted in the tertiary referral center of Verona Hospital between June 2014 and June 2020. Patient demographics, clinical and surgical data were assessed. Speech objective and subjective evaluation was performed. QoL was assessed with Voice Handicap Index-10 (VHI-10) and M.D. Anderson Dysphagia Inventory (MDADI) questionnaires. RESULTS: Seven patients met the inclusion criteria, but two had died before questionnaires were administered. No intraoperative complications were noted after TDP surgery. There were delayed complications in four cases (57.1%) and all were treated with restoration. Intelligible voice was restored in all patients. Maximum phonation time and maximal voice intensity recorded were 5.42 ± 3.27 s and 65.20 ± 5.45 dB, respectively. Acceptable average VHI-10 and MDADI scores were obtained in all patients. CONCLUSIONS: Secondary TDP performed after gastric pull-up or colon transposition reconstructions are feasible and effective procedures. An intelligible voice was restored in all patients, with satisfactory patient-perceived voice-related and dysphagia-related quality-of-life outcomes. PMID:35034188 | DOI:10.1007/s00405-022-07251-8 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Complications during Fiberoptic Endoscopic Evaluation of Swallowing (FEES) in 5680 examinations

xlomafota13 shared this article with you from Inoreader Complications during Fiberoptic Endoscopic Evaluation of Swallowing (FEES) in 5680 examinations Via Folia Phoniatrica et Logopaedica Objective: To evaluate retrospectively the incidence of complications during Fiberoptic Endoscopic Evaluation of Swallowing (FEES) in 5680 examinations. Patients and Methods: 5680 patients were evaluated at the Department of Otorhinolaryngology, Audiology and Phoniatrics of Pisa University Hospital between January 2014 and December 2018, involving both inpatients and outpatients. Most common comorbidities included neurological pathologies such as stroke (11.8%), neurodegenerative diseases (28.9%) and a history of previous head and neck surgery (24.6%). The evaluation was conducted by clinicians with experience in swallowing for a minimum of 10 years with the assistance of one or more speech language pathologists. Results: In all patients studied the endoscope insertion was tolerated and it was possible to visualize the pharyngo-laryngeal structures. Most patients reported discomfort In a minority of patients, complications were recorded, such as anterior epistaxis, posterior epistaxis, vasovagal crises and laryngospasm. Laryngospasm was recorded in patients affected by Amyotrophic Lateral Sclerosis (ALS). Multivariate binary logistic regression showed that discomfort, chronic gastrointestinal diseases, neurodegenerative diseases and brain tumors were risk factors associated with minor complications. Conclusions: FEES proved to be easy to perform, well tolerated by the patients and cost-effective. It can be performed at the patient's bedside and it is characterized by low rate of complications. As a matter o f fact, normally only discomfort, gagging and/or vomit are reported. Only rarely complications occur, such as anterior or posterior epistaxis episodes or vasovagal crises, but these are still easily managed. Exceptionally, more severe complications are reported: adverse drug reactions to substances such as blue dye (methylene blue) and local anesthetics (not used in our protocol), and laryngospasm. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

How do we get around the barriers to integrating palliative care in oncology?

xlomafota13 shared this article with you from Inoreader How do we get around the barriers to integrating palliative care in oncology? Via Association française pour l'étude du cancer Bull Cancer. 2022 Jan 13:S0007-4551(21)00684-6. doi: 10.1016/j.bulcan.2021.12.006. Online ahead of print. ABSTRACT Supported by numerous scientific publications showing its clinical benefits, early palliative care has become a gold standard in oncology since 2017, recommended for patients with advanced cancer by the major societies of oncology. Nevertheless, palliative care team integration is still too late in France and the intervention of palliative care teams in oncology is still often limited to the management of patients and their relatives at the end of life. First, we will look at the main obstacles: the lack of staff in palliative care teams and the complex functioning of palliative care identified beds; also, the difficulties of communication with the patient and his relatives for the introduction of palliative care. We will then discuss the prospects for development, moving from the concept of early palliative care (systemat ic from the advanced phase) to integrated palliative care (targeted to patients' needs). Standardization of the integrated palliative care pathway requires the description of referral criteria, screening modalities, different clinical missions, and collaboration modalities with oncologists. Palliative care and oncology teams, working together, can enable holistic medicine that focuses on the needs of patients and their loved ones, giving voice to their preferences and aiming to improve their quality of life. PMID:35034784 | DOI:10.1016/j.bulcan.2021.12.006 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

A Rare Cause of Secondary Otalgia

xlomafota13 shared this article with you from Inoreader Message: PrivacyPrivacy Skip to main content Intended for healthcare professionals SAGE Journals Search search-icon Browse Resources Access Options: You are not signed in to your personal profile Sign In You are not signed in via an institution Institution You are not authenticated via a society Society Cart Ear, Nose & Throat Journal 1.697 Impact Factor 5-Year Impact Factor 1.745 Journal Indexing & Metrics » Journal Home Browse Journal Journal Info Stay Connected Submit Paper Search Article Menu Download PDF [PDF] Open EPUB Full Article Content List Significance Statement ORCID iDs References Figures & Tables Article Metrics Cite Share Request Permissions Related Articles A Rare Cause of Secondary Otalgia Show all authors Evropi Forozidou, MD, Nikolaos Tsetsos, MD, MSc, Paraskevi Karamitsou, MD, MSc, ... First Published January 17, 2022 Research Article https://doi.org/10.1177/01455613221075226 Article information Open AccessCreative Commons Attribution, Non Commercial 4.0 License Significance Statement Secondary otalgia is defined as pain felt in the ear although originating from a non-otologic source. The complex innervation of ear structures makes the identification of the responsible region a challenging procedure. The 2 most common causes of secondary otalgia are the temporomandibular joint dysfunction and dental infections. We present a rare case of secondary otalgia caused by a foreign body hidden deeply in the lateral surface of the tongue. A 61-year-old male ironworker presented to our emergency Ear, Nose and Throat department complaining about left otalgia accompanied by difficulty in swallowing. Symptoms had started 1 week before in his work environment. The patient was prescribed a 5-day course of antibiotics with ciprofloxacin ear drops combined with painkillers by his family doctor without, however, any signs of improvement. His past medical history was otherwise normal. A thorough clinical examination combined with otomicroscopy was unremarkable for any ear pathology. Fiberoptic nasolaryngoscopy and laboratory tests were also normal. Inspection of the oral cavity showed no signs of inflammation; however, a tender area on the left lateral surface of the tongue was noted. After careful observation, a tiny hole was recognized in the same area (Figure 1). An exploration of the area under local anesthesia was conducted and a metallic iron bar of approximately 1.5 cm in length was removed (Figure 2). Symptoms were completely subsided and the patient remained pain free at 1-week follow-up. figure Figure 1. Oral cavity inspection. Recognition of the painful area on the left lateral surface of the tongue. figure Figure 2. The extracted foreign body. A metallic iron bar. Otalgia is a rather common symptom seen in the primary care setting with many diverse causes. Primary otalgia is related to clinical entities affecting the outer, middle, and inner ear.1 Inflections such as acute or chronic media otitis, external otitis, folliculitis, mastoiditis, and myringitis constitute the most common etiologic factors. Cerumen obstruction, ear neoplasms, and trauma may also be responsible for primary otalgia. The origin of primary otalgia is almost always easy to be established with otomicroscopy or radiographic imaging.2 On the other hand, when the cause of pain cannot be localized to the affected ear, it is referred to as secondary otalgia. There is a considerable overlap between the innervation of the ear and the related areas in the head and neck. Innervation of the ear structures comprises multiple lower cranial, upper cervical, and peripheral nerves. They innervate the spine, skull base, salivary glands, pharynx, larynx, oral cavity, orbits, face, paranasal sinuses, and deep neck spaces. The most common causes of secondary otalgia are temporomandibular joint syndrome and dental infections. Additionally, other potential causes of otalgia are Bell's palsy, salivary gland disorders, pharyngitis, tonsillitis, oral disorders, and cervical osteoarthritis.2,3 Clinicians should be aware that otalgia could be the primary symptom of a head and neck malignancy. Therefore, a thorough clinical examination of the whole head and neck area is imperative to exclude neoplasms.3,4 Inflammation, trauma, and neoplasms of the tongue often cause secondary otalgia via the trigeminal (CN V) and the glossopharyngeal nerve (CN IX). The third branch of the trigeminal, the mandibular nerve (V3), is a mixed nerve. The auriculotemporal nerve is a branch of the V3 that provides sensation to the anterosuperior pinna, anterior external auditory canal, and the anterior lateral aspect of the tympanic membrane. Other branches include the lingual, buccal, and inferior alveolar nerves that provide sensory innervation to the oral cavity, the floor of the mouth, and the anterior two-thirds of the tongue.5 The glossopharyngeal nerve (CN IX) directly innervates the inner surface of the tympanic membrane as well as the middle ear cavity through sensory fibers of the tympanic nerve (Jacobson nerve). It also provides mixed innervation to the posterior third of the tongue.6 Secondary otalgia may be caused from anywhere along the course of this nerve. In cases that thorough clinical investigation fails to establish the source of otalgia, a computed tomography or magnetic resonance imaging studies should be considered to define the diagnosis.5 Declaration of Conflicting Interests The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. Funding The author(s) received no financial support for the research, authorship, and/or publication of this article. ORCID iDs Nikolaos Tsetsos https://orcid.org/0000-0003-1884-6824 Konstantinos Garefis https://orcid.org/0000-0003-3905-5650 Alexandros Poutoglidis https://orcid.org/0000-0002-4591-8347 References 1. Neilan, RE, Roland, PS. Otalgia. Med Clin North Am. 2010;94:96171. Google Scholar | Crossref 2. Norris, CD, Koontz, NA. Secondary Otalgia: Referred Pain Pathways and Pathologies. AJNR Am J Neuroradiol. 2020;41(12):2188-2198. Google Scholar | Crossref | Medline 3. Earwood, JS, Rogers, TS, Rathjen, NA. Ear pain: diagnosing common and uncommon causes. Am Fam Physician. 2018;97(1):20-27. Google Scholar | Medline 4. Charlett, SD, Coatesworth, AP. Referred otalgia: a structured approach to diagnosis and treatment. Am J Med Sci Med. 2017;5(3):56-61. Google Scholar 5. Scarbrough, TJ, Day, TA, Williams, TE, et al. Referred otalgia in head and neck cancer: a unifying schema. Am J Clin Oncol. 2003;26:e157-e162. Google Scholar | Crossref | Medline 6. Naraev, BG, Linthicum, FH. Traumatic neuroma of the tympanic (Jacobson's) nerve as a possible cause of otalgia. Otolaryngol Head Neck Surg. 2008;138:735-737. Google Scholar | SAGE Journals View Abstract SAGE Recommends Also from SAGE Publishing CQ LibraryAmerican political resourcesopens in new tab Data PlanetA universe of dataopens in new tab Lean LibraryIncrease the visibility of your libraryopens in new tab SAGE Business CasesReal-world cases at your fingertipsopens in new tab SAGE CampusOnline skills and methods coursesopens in new tab SAGE KnowledgeThe ultimate social science libraryopens in new tab SAGE Research MethodsThe ultimate methods libraryopens in new tab SAGE VideoStreaming video collectionsopens in new tab SAGE Journals About Privacy Policy Terms of Use Contact Us Help Accessibility Browse Health Sciences Life Sciences Materials Science & Engineering Social Sciences & Humanities Journals A-Z Discipline Hubs Resources Authors Editors Reviewers Librarians Researchers Societies Opportunities Advertising Reprints Content Sponsorships Permissions Microsites Ear, Nose & Throat Journal ISSN: 0145-5613 Online ISSN: 1942-7522 Copyright © 2022 by SAGE Publications A Rare Cause of Secondary Otalgia Via ear nose throat Ear Nose Throat J. 2022 Jan 17:1455613221075226. doi: 10.1177/01455613221075226. Online ahead of print. NO ABSTRACT PMID:35037504 | DOI:10.1177/01455613221075226 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Comparison of endoscopic over-underlay technique with and without packing for repairing chronic perforation

xlomafota13 shared this article with you from Inoreader Comparison of endoscopic over-underlay technique with and without packing for repairing chronic perforation Via http://link.springer.com/journal/405,European archives of oto-rhino-laryngology,The European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino Eur Arch Otorhinolaryngol. 2022 Jan 17. doi: 10.1007/s00405-022-07254-5. Online ahead of print. ABSTRACT OBJECTIVE: This study was performed to compare the efficacy of the endoscopic modified cartilage over-underlay technique with and without packing for repairing chronic tympanic membrane (TM) perforations. METHODS: A total of 70 cases of chronic TM perforation were randomly allocated to endoscopic modified cartilage over-underlay myringoplasty groups with (n = 35) and without (n = 35) packing. The graft success rate and hearing outcomes were compared between the two groups. In addition, neovascularization scores were subjectively obtained. RESULTS: At 12 months postoperatively, the difference in graft success rate between the packing and no-packing groups was not significant (94.3% vs. 100.0%, P = 0.473). In addition, there were no significant differences between the two groups in the pre- or postoperative air-bone gap (A BG) (15.18 ± 2.73 vs. 15.07 ± 4.02, P = 0.623 and 8.63 ± 3.03 vs. 8.52 ± 4.50, P = 0.591) or mean ABG gain (6.56 ± 3.23 vs. 6.54 ± 2.83, P = 0.751). However, the average operating times were 43.6 ± 7.1 and 32.7 ± 2.1 min in the packing and no-packing groups, respectively (P < 0.001). CONCLUSIONS: Surgical and hearing outcomes were comparable between patients with chronic TM perforation treated using the endoscopic modified over-underlay technique with and without packing. However, without packing, the procedure was less invasive and had a shorter operating time. PMID:35038027 | DOI:10.1007/s00405-022 -07254-5 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Benefit on daily listening with technological advancements: comparison of basic and premium category hearing aids

xlomafota13 shared this article with you from Inoreader Benefit on daily listening with technological advancements: comparison of basic and premium category hearing aids Via http://link.springer.com/journal/405,European archives of oto-rhino-laryngology,The European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino Eur Arch Otorhinolaryngol. 2022 Jan 17. doi: 10.1007/s00405-021-07240-3. Online ahead of print. ABSTRACT PURPOSE: The aim of the study was to compare the user-rated benefit of two categories of hearing aids, mainly the basic and premium categories of hearing aids. METHODS: A questionnaire was administered on 102 hearing aids users (47 basic and 55 premium category users) with severity of hearing loss ranging from mild to moderately severe sensorineural hearing loss. The questionnaire administered was divided into mainly seven subscales which included speech intelligibility in quiet and in noise, ease of communication, the efficiency of noise reduction, localization, quality of music perception and cost effectiveness. The effect of duration of daily usage of hearing aids on performance among these different subscales was also assessed. RESULTS: Ease of communication was rated better by premium hearing aid users, whereas the cost effectiveness was rated to be better by basic users. There was no significant difference observed between performances of basic versus premium category of hearing aids in other listening domains assessed. There was no significant difference in any of the listening domains with daily usage duration for both categories of hearing aid users. CONCLUSION: The users of premium category devices revealed better ease of communication in daily environments, whereas performance of these devices on other listening domains remains questionable. Cost effectiveness was reported to be better by the users of basic hearing aids. A prospective and controlled paired series comparison of hearing aid performance needs to be performed to confirm these findings. PMID:35038028 | DOI:10.1007/s00405-021-07240-3 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.