Αρχειοθήκη ιστολογίου

Αλέξανδρος Γ. Σφακιανάκης
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5
Άγιος Νικόλαος Κρήτη 72100
2841026182
6032607174

Δευτέρα 13 Φεβρουαρίου 2017

Differences in eosinophil molecular profiles between children and adults with eosinophilic esophagitis

Abstract

Background

Eosinophilic esophagitis (EoE) afflicts both children and adults. It has been debated whether pediatric and adult EoE represent different disease entities. The objectives of this study were to determine if the blood eosinophil molecular pattern of children with EoE is: 1) distinct from that of healthy children; and 2) different from that of adults with EoE.

Methods

Blood eosinophils from children and adults with EoE, and healthy controls, were analyzed with flow cytometry regarding levels of CD23, CD44, CD54, CRTH2, FOXP3 and galectin-10. Eosinophil FOXP3 and galectin-10 mRNA levels were determined by qPCR. The data was analyzed by using a multivariate method of pattern recognition.

Results

An eosinophil molecular pattern capable of distinguishing children with EoE from control children was identified. A smaller fraction of eosinophils from children with EoE expressed CD44 and a larger fraction expressed CRTH2 than the controls. Eosinophils from children with EoE also had higher levels of galectin-10 mRNA and lower levels of FOXP3 mRNA. The eosinophils from children with EoE had lower levels of surface CD54 and of FOXP3 mRNA compared with the eosinophils from the adult patients. A key finding was the detection in healthy individuals of age-related differences in the levels of several eosinophil markers.

Conclusions

Children with EoE can be distinguished from healthy children based on the molecular patterns of their blood eosinophils. Age-related physiologic differences in eosinophil molecular patterns may partly explain the different blood eosinophil phenotypes in children versus adults with EoE.

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Phase I trial of low-dose interleukin 2 therapy in patients with Wiskott-Aldrich syndrome

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Publication date: Available online 14 February 2017
Source:Clinical Immunology
Author(s): Soma Jyonouchi, Brenda Gwafila, Lavesh A. Gwalani, Maria Ahmad, Chistopher Moertel, Cecil Holbert, Ji Young Kim, Nathan Kobrinsky, Sumita Roy-Ghanta, Jordan S. Orange
BackgroundLow dose IL-2 can restore the function of T and NK cells from Wiskott-Aldrich (WAS) patients. However, the safety of in vivo IL-2 in WAS is unknown.ObjectivesA phase-I study to assess safety of low dose IL-2 in WAS.MethodsPatients received 5 daily subcutaneous IL-2 injections, every 2months, for three courses. A "3+3" dose escalation method was used.Results6 patients received the 0.5millionunits/m2/day dose without serious adverse events. However, 2 of 3 patients receiving the 1millionunits/m2/day dose developed thrombocytopenia requiring platelet transfusions. A statistically significant platelet increase occurred in patients receiving the 0.5millionunits/m2/day dose. A trend toward higher T, B and NK cell numbers and higher T regulatory cell percentages was observed.ConclusionWe have identified a safe IL-2 dose for WAS patients. Additional trials are indicated to study the efficacy of this immunostimulant as a therapy for WAS.



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Decision Support for the Capacity Management of Bronchoscopy Devices: Optimizing the Cost-Efficient Mix of Reusable and Single-Use Devices Through Mathematical Modeling.

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BACKGROUND: Increasing costs of material resources challenge hospitals to stay profitable. Particularly in anesthesia departments and intensive care units, bronchoscopes are used for various indications. Inefficient management of single- and multiple-use systems can influence the hospitals' material costs substantially. Using mathematical modeling, we developed a strategic decision support tool to determine the optimum mix of disposable and reusable bronchoscopy devices in the setting of an intensive care unit. METHODS: A mathematical model with the objective to minimize costs in relation to demand constraints for bronchoscopy devices was formulated. The stochastic model decides whether single-use, multi-use, or a strategically chosen mix of both device types should be used. A decision support tool was developed in which parameters for uncertain demand such as mean, standard deviation, and a reliability parameter can be inserted. Furthermore, reprocessing costs per procedure, procurement, and maintenance costs for devices can be parameterized. RESULTS: Our experiments show for which demand pattern and reliability measure, it is efficient to only use reusable or disposable devices and under which circumstances the combination of both device types is beneficial. CONCLUSIONS: To determine the optimum mix of single-use and reusable bronchoscopy devices effectively and efficiently, managers can enter their hospital-specific parameters such as demand and prices into the decision support tool. The software can be downloaded at: http://ift.tt/2lejUcI. (C) 2017 International Anesthesia Research Society

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Peristomal psoriasis

Summary

Background

Psoriasis is a common skin condition, affecting 1.3–2.2% of the population. The prevalence of psoriasis has previously been reported as 11.2% in patients with Crohn disease (CD) and 5.7% in patients with ulcerative colitis.

Aim

To assess the prevalence of psoriasis around stoma sites and identify the most effective treatment.

Methods

A departmental database of all patients attendings stoma clinic was accessed between 1 May 2003 and 15 October 2015. Psoriasis response was determined by clinical resolution of psoriatic plaques in terms of erythema, induration, scaling and stoma bag adherence.

Results

Of 1665 patients, 78 (4.7%) had psoriasis affecting their abdominal stoma. In 8 patients (11.5%), a thicker hydrocolloid stoma bag barrier was sufficient to resolve the peristomal psoriasis. For 75 patients (96.2%), initial management was with nongreasy topical steroid scalp preparations and their usual barrier in the form of their stoma bag. Patients who did not respond to topical treatments initially or who experienced secondary failure (n = 16; 20.5%) received different systemic treatments [ciclosporin (n = 2), methotrexate (n = 2; 1 patient did not respond) or ultraviolet B narrowband phototherapy (n = 8; 2 nonresponders). One patient received infliximab and four patients received adalimumab for treatment of their CD, and their peristomal psoriasis also responded to these treatments. A further patient was prescribed adalimumab by the dermatology departmetn for the management of psoriasis vulgaris, with good response.

Conclusion

Peristomal psoriasis is common but potentially under-recognized. Selecting effective treatment that does not hinder bag appliance is crucial for effective management. Effective treatments include hydrocolloid dressings and topical corticosteroid regimens.



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Verrucous discoid lupus erythematosus in a child



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Immune checkpoint receptors in cancer: redundant by design?

Jing Li | Ling Ni | Chen Dong

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Dendritic cells in cancer: the role revisited

Filippo Veglia | Dmitry I Gabrilovich

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Complete Obstruction of Endotracheal Tube in an Infant with a Retropharyngeal and Anterior Mediastinal Abscess

Intraoperative ventilatory failure is not an uncommon complication; however, acute endotracheal obstruction by a foreign body or blood clot can be difficult to quickly discriminate from other causes. Once the diagnosis is made, quick action is needed to restore ventilation. The ultimate solution is to exchange the endotracheal tube; however, there can be other ways of resolving this in situations where reintubation would be difficult or unsafe. This case report discusses such an event in an infant with multiple airway challenges including a retropharyngeal and anterior mediastinal abscess. We have also formulated a pathway based on various case reports involving complete ETT obstruction.

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Beneficial Effects on Pregnancy Outcomes of Thyroid Hormone Replacement for Subclinical Hypothyroidism

Background. Hypothyroidism and raised thyroid antibody levels have been associated with adverse obstetrical outcomes. Several studies have investigated causal associations, but results have been inconsistent and few studies have reported the effects of thyroxine replacement therapy on pregnancy outcomes in hypothyroid patients. Objective. The primary study objective was to determine the outcome of pregnancies in women diagnosed with overt and subclinical hypothyroidism (SCH) (serum TSH > 2.5 mIU/L) and those with elevated circulating thyroid autoantibody levels in the first trimester of pregnancy and after the institution of appropriate thyroxine replacement therapy to maintain the serum TSH ≤ 2.5 mIU/L. Study Design. This prospective observational study was undertaken between 2013 and 2016. Blood samples were taken from 1025 women at presentation for thyroid stimulating hormone (TSH), anti-thyroglobulin antibodies (TGAb), and thyroid peroxidase antibodies (TPOAb). Those with a TSH > 2.5 mIU/L were treated with thyroxine and managed appropriately to ensure that the TSH was maintained ≤2.5 mIU/L. Outcomes in these patients were compared to those in euthyroid patients. Maternal antenatal complications and perinatal outcomes were recorded. Results. There were a total of 1025 patients of whom 382 (37.5%) were nulliparous. 10.1% had a TSH level > 2.5 mIU/L and 18.2% had at least one raised thyroid antibody level. No differences in adverse outcomes of pregnancy were evident in women treated for SCH or overt hypothyroidism compared to the euthyroid group. There was also no association between raised thyroid antibodies and adverse pregnancy outcomes in either group. Conclusion. There were no adverse outcomes of pregnancy found in pregnant women who had been diagnosed and treated with thyroxine for SCH at the time of presentation when compared to euthyroid patients. There was also no relationship with thyroid antibodies and adverse pregnancy outcomes in the two groups. It is not possible to unequivocally advocate for thyroxine replacement in pregnant women with subclinical and overt hypothyroidism until large scale randomized controlled trials are performed.

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Formal mentorship in a surgical residency training program: a prospective interventional study

Otolaryngology-Head and Neck surgery resident physicians (OHNSR) have a high prevalence of burnout, job dissatisfaction and stress as shown within the literature. Formal mentorship programs (FMP) have a proven...

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Developing a new diagnostic algorithm for human papilloma virus associated oropharyngeal carcinoma: an investigation of HPV DNA assays

Human papilloma virus (HPV) has been implicated in the development of a large proportion of oropharyngeal squamous cell carcinoma (OPSCC). Current techniques used to diagnose HPV etiology require histopatholog...

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Does drug-induced sleep endoscopy change the surgical decision in surgically naïve non-syndromic children with snoring/sleep disordered breathing from the standard adenotonsillectomy? A retrospective cohort study

Adenotonsillectomy is the most commonly performed operation for pediatric snoring/sleep disordered breathing (S/SDB). However, 20–40% of patients will fail to improve. Drug-induced sleep endoscopy (DISE) may p...

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Merkelzellkarzinom: kutane Manifestation einer hochmalignen Prä-/pro-B-Zell-Neoplasie?

Zusammenfassung

Das Merkelzellkarzinom (MZK) ist eine relativ seltene, jedoch hochmaligne neoplastische Proliferation der Haut, die v. a. bei älteren und immunsupprimierten Patienten vorkommt. Die Identifizierung des Merkelzell-Polyomavirus (MCPyV) 2008 hat das Verständnis der Ätiopathogenese des MZK grundlegend verändert. Etwa 80 % der MZK sind MCPyV-positiv, und das Virus ist zumeist klonal in die Tumor-DNA integriert. Die kürzlich veröffentlichen Ergebnisse klinischer Studien zur Blockade des „PD-1 immune checkpoint pathway" sind vielversprechend für zukünftige Therapieoptionen des MZK. Trotz dieser grundlegenden Erkenntnisgewinne bleibt der zelluläre Ursprung des MZK bis heute unbekannt. Aufgrund der Histologie, Genexpression und molekularer Analysen haben wir kürzlich die Hypothese formuliert, dass MZK ihren zellulären Ursprung in Prä-/pro-B-Zellen nehmen. In dieser Arbeit werden die derzeitigen Konzepte zum zellulären Ursprung des MZK diskutiert, d. h. die Merkelzelle, die (epi)dermale Stammzelle und die Prä‑/pro-B-Zelle. Der Fokus dieser Arbeit liegt auf dem Konzept der Prä‑/pro-B-Zellen als zellulärer Ursprung der MZK, da dieses – nach Meinung der Autoren – möglicherweise auch als Grundlage dienen könnte zum besseren Verständnis anderer kleinzelliger Tumoren unbekannten zellulären Ursprungs, z. B. kleinzellige Karzinome der Lunge, und anderer anatomischer Lokalisationen. Zudem eröffnet es möglicherweise neue Therapieoptionen für klinisch weit fortgeschrittene MZK.



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Paravirale Exantheme

Zusammenfassung

Unter paraviralen Exanthemen sind distinkte Hauterkrankungen aufzufassen, die auf Infektionen mit unterschiedlichen Viren zurückzuführen sind. Auch wenn sich bisher bei einigen Exanthemerkrankungen kein Virus identifizieren ließ, sprechen doch das Manifestationsalter der Patienten, der klinische Verlauf des Exanthems und extrakutane Symptome für eine virale Genese. Während viele Virusexantheme direkte Folge der Infektion darstellen, reflektieren paravirale Exantheme die Antwort des Immunsystems auf die Infektionserreger. Viren lassen sich dann nicht in der Haut nachweisen. Zu typischen paraviralen Exanthemen gehören Gianotti-Crosti-Syndrom, Pityriasis rosea, Pityriasis lichenoides, „papular-purpuric gloves and socks syndrome" sowie das asymmetrische periflexurale Exanthem. Das unilaterale mediothorakale Exanthem und die eruptive Pseudoangiomatose sind selten, und die eruptive Hypomelanose wurde erst kürzlich beschrieben.



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Placeboeffekt in Studien zur allergenspezifischen Immuntherapie mit Inhalationsallergenen

Zusammenfassung

Placeboeffekte spielen bei der Therapie allergischer Erkrankungen eine wesentliche Rolle. In dieser Arbeit wurden die doppelblind-placebokontrollierten Studien zur allergenspezifischen Immuntherapie (ASIT) mit Inhalationsallergenen (Birke, Gräser, Milben), die in den im Juli 2016 aktualisierten Tabellen zur deutschen S2k-Leitlinie zur ASIT IgE-vermittelter Erkrankungen aufgelistet werden, gezielt hinsichtlich ihrer beschriebenen Placeboeffekte analysiert. Als Placebo wurde meist Verum ohne Allergen eingesetzt, bei subkutaner Applikation teilweise Histamin zugefügt. Detaillierte Aussagen zum reinen Placeboeffekt sind in keiner der untersuchten 33 Studien zu finden. Lediglich in einer Studie wurde der Symptom-Medikations-Score (SMS) in einer adäquaten Baseline-Periode untersucht. Ein unbehandelter Arm war in keiner Studie zu finden. Indirekte Hinweise zeigen bei retrospektiven, subjektiven Parametern deutliche Placeboeffekte bei bis zu 77 % der Probanden. Bekannte Einflussfaktoren auf den Placeboeffekt wie Alter, Geschlecht, Applikationsart/Zusammensetzung des Placebos, individuelle und kulturelle Unterschiede, Symptomschwere zu Beginn und Wahrscheinlichkeit, das Verum zu erhalten, wurden nicht adressiert und konnten anhand der verfügbaren Daten nicht bewertet werden. Zusammenfassend wurde der Placeboeffekt in ASIT-Studien bisher nicht ausreichend untersucht. Trotz des erheblichen Zeit- und Kostenaufwands sollten zukünftige ASIT-Studien den SMS in einer adäquaten Baseline-Periode vor Studienbeginn erheben und möglichst einen unbehandelten Vergleichsarm integrieren. Ein besseres Verständnis der Placeboeffekte in Studien zur allergenspezifischen Immuntherapie wird zu einem optimierten Studiendesign und einer verbesserten Einschätzung des Therapieeffekts führen.



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Effect of Vitamin D Deficiency on the laryngo-pharyngeal Tract

To investigate the effect of vitamin D deficiency on the laryngopharyngeal tract.

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Bilateral Sinonasal Extramedulary Plasmacytoma treated with radiotherapy and a Medial Maxillectomy with a Denker's procedure

Extramedullary plasmacytoma (EMP), multiple myeloma (MM), and solitary plasmacytoma of the bone (SPB) are each classified as distinct manifestations of a disease continuum with varying symptoms, treatment regimens, and prognostic implications. While the etiology for each of these neoplastic entities is due to a derangement of plasma cells, these B-cell neoplasms are indistinguishable histologically [1] [1]. EMP, a rare neoplasm, accounts for approximately 2–3% of all plasma cell tumors, SPB accounts for up to 4–6% of cases, and multiple myeloma for 91–94% [1] [2] [3].

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Lobular capillary hemangiomas: Case report and review of literature of vascular lesions of the nasal cavity

Vascular tumors of the nasal cavity can represent a variety of pathologies. In this case report, we discuss two patients presenting with a large vascular lesion occupying the nasal cavity. Significant bleeding was encountered during the initial attempts for endoscopic surgical resection. One lesion was successfully excised following preoperative embolization while a second following sphenopalatine artery ligation vascular ligation. In both cases, final pathology showed lobular capillary hemangioma (LCH).

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Is histopathological diagnosis useful in choosing treatments for tumors of the temporal bone?

We were pleased to read the article by Olcott C. andStrasnick B. titled "A blue middle ear mass: Cholesterol granuloma mimicking a glomus tumor and endolymphatic sac tumor". Am J Otolaryngol.2017;38(1):100-102 [1], which presents very important clinical observations. Based on our experience and the literature review, we would like to present some comments on this problem. The benign tumors of the temporal bone (TB) have been divided into three groups: cholesterol granuloma (CG), glomus tumor (GT) and endolymphatic sac tumor (ELST) [1].

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The diagnostic accuracy of a laser fluorescence device and digital radiography in detecting approximal caries lesions in posterior permanent teeth: an in vivo study

Abstract

The aim of this in vivo study was to test the diagnostic accuracy of a pen-type laser fluorescence (LFpen) device in detecting approximal caries lesions, in posterior permanent teeth, at the cavitation and non-cavitation thresholds, and compare it with that of digital bitewing radiography. Thirty patients (aged 18–37), who attended the Faculty of Dentistry at Damascus University for a dental examination, were consecutively screened. Ninety approximal surfaces of posterior permanent teeth without frank cavitations, enamel hypoplasia or restorations were selected and examined using the LFpen (DIAGNOdent pen) and digital bitewing radiography. The reference standard was the visual-tactile inspection, after performing temporary tooth separation, using orthodontic rubber rings, placed for 7 days. The status of included approximal surfaces was recorded as intact/sound, with white/brown spots or cavitated. One trained examiner performed all examinations. There were statistically significant differences in LFpen readings between the three types of approximal surface status (P < 0.001). The optimal cut-off values for detecting approximal caries lesions in posterior permanent teeth were >16 and 8 at the cavitation and non-cavitation thresholds respectively. The sensitivity, specificity and accuracy (measured by the area under the receiver-operating characteristic curve) were 100, 85 and 95 and 92, 90 and 95% at the cavitation and non-cavitation thresholds respectively. The intra-class correlation coefficient for intra-examiner reliability was 0.95. The diagnostic accuracy of the LFpen was significantly higher than that of digital bitewing radiography (P < 0.001). The LFpen's diagnostic performance was accurate and significantly better than digital bitewing radiography in detecting approximal caries lesions, in posterior permanent teeth.



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Tinnitus – Klinik und Therapie

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Laryngo-Rhino-Otol 2017; 96: 47-59
DOI: 10.1055/s-0042-119419

Tinnitus ist ein Phantomgeräusch, das ein Symptom verschiedener Störungen sein kann, z. B. durch Hörverlust, kardiovaskuläre oder neurologische Erkrankungen, Diabetes oder Tumoren. Bei dekompensiertem Tinnitus leiden die Patienten aufgrund ihrer Unfähigkeit, die auditive Aufmerksamkeit vom Tinnitus-Ton abzulenken, an Schlaflosigkeit, Konzentrationsproblemen und anderen psychologischen Störungen. In extremen Fällen kann es zu Symptomen einer manifesten Depression kommen. Der auditorische Charakter des Tinnitus veranlasst die Patienten überwiegend, Hilfe bei HNO-Ärzten zu suchen. Das erste Ziel der Behandler besteht darin, die Ursache des Tinnitus zu bestimmen und seine audiologischen Eigenschaften zu messen. Das zweite Ziel ist es, die tinnitusinduzierte Belastung einzuschätzen, den Schweregrad zu bewerten und u. U. weiterführende Therapien einzuleiten. Der Artikel beschreibt die audiologischen und psychologischen Eigenschaften des Tinnitus, derzeitige Klassifikationsmodelle und die therapeutischen Ansätze auf diesem Gebiet.
[...]

© Georg Thieme Verlag KG Stuttgart · New York

Article in Thieme eJournals:
Table of contents  |  Abstract  |  CME online  |  Full text



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Eosinophile Ösophagitis – Neues zur Pathogenese, Diagnostik und Therapie

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Laryngo-Rhino-Otol 2017; 96: 11-21
DOI: 10.1055/s-0042-119417

Die eosinophile Ösophagitis (EoE) ist eine Krankheitsentität, der man erst im letzten Jahrzehnt eine erhöhte Aufmerksamkeit gewidmet hat und seitdem wird sie mit deutlich erhöhter Frequenz diagnostiziert. Sie stellt eine chronisch immunvermittelte Entzündung des Ösophagus dar. Bei Erwachsenen ist die Dysphagie das Leitsymptom. Die Diagnose wird durch die Ösophago-Gastro-Duodenoskopie mit Entnahme von Stufenbiopsien aus dem Ösophagus gestellt. Die charakteristischen histopathologischen Veränderungen mit eosinophilen Infiltraten der ösophagealen Mukosa sind der diagnostischen Goldstandard. Bislang gibt es weltweit keine zugelassene Therapie für die EoE, auch wenn topische und systemisch wirkende Kortikosteroide eine hohe Wirksamkeit zeigen. Weitere therapeutische Optionen stellt die Eliminationsdiät und in ausgewählten Fällen die endoskopische Dilatation dar.
[...]

© Georg Thieme Verlag KG Stuttgart · New York

Article in Thieme eJournals:
Table of contents  |  Abstract  |  Full text



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Changes in bite force after orthognathic surgical correction of mandibular prognathism: a systematic review

Patients requesting treatment for mandibular prognathism seek functional and aesthetic improvements. Improvements in bite force and efficiency are generally used as measures of better function. It is unclear what effect the surgical correction of mandibular prognathism will have on the patient's occlusal forces. The literature was searched using medical subject heading (MeSH) and key word terms 'bite force', 'osteotomy', 'orthognathic surgery', and 'prognathism'. A total of 17 articles were included in this review.

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Expression pattern of heat shock protein 90 in patients with oral squamous cell carcinoma in northern Taiwan

Heat shock protein 90 (HSP90), which is expressed in cancer cells, profoundly affects progression, invasion, and metastasis. However, to our knowledge, in East Asia, the correlation between the expression of HSP90 and clinicopathological variables has seldom been discussed. We therefore investigated this and its prognostic value in 36 patients newly diagnosed with oral squamous cell carcinoma (SCC) in northern Taiwan. Samples of tumour and normal samples from the patients were compared immunohistochemically.

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SOP – Akuter Verwirrtheitszustand



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SOP – Umgang mit und Versorgung von Verstorbenen



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Undifferentiated “round” cell (“Ewing-like”) sarcoma: not always so-round nor Ewing-like



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Toward optimal processing of endoscopic submucosal dissection specimens



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Scaling up strategies of the chronic respiratory disease programme of the European Innovation Partnership on Active and Healthy Ageing – Executive summary

Publication date: Available online 13 February 2017
Source:Alergologia Polska - Polish Journal of Allergology
Author(s): Th. Werfel, P. Hellings, I. Agache, A. Muraro, J. Bousquet
Action Plan B3 of the European Innovation Partnership on Active and Healthy Ageing (EIP on AHA) focuses on the integrated care of chronic diseases. Area 5 (Care Pathways) was initiated using chronic respiratory diseases as a model. The chronic respiratory diseases action plan involves AIRWAYS integrated care pathways (ICPs). It is deployed in collaboration with the WHO Global Alliance against Chronic Respiratory Diseases (GARD). The EIP on AHA has proposed a 5-step framework for developing an individual scaling up strategy: (i) what to scale up: (i-a) databases of good practices, (i-b) assessment of viability of the scaling up of good practices, (i-c) classification of good practices for local replication and (ii) how to scale up: (ii-a) facilitating partnerships for scaling up, (ii-b) implementation of key success factors and lessons learnt, including emerging technologies for individualised and predictive medicine. This strategy has already been applied to the chronic respiratory diseases action plan of the EIP on AHA.



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Preserving brain function in a comatose patient with septic hyperpyrexia (41.6 °C): a case report

Pyrexia is a physiological response through which the immune system responds to infectious processes. Hyperpyrexia is known to be neurodegenerative leading to brain damage. Some of the neurotoxic effects of hy...

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Association of ORMDL3 with rhinovirus-induced endoplasmic reticulum stress and type I Interferon responses in human leukocytes

Abstract

Background

Children with risk alleles at the 17q21 genetic locus who wheeze during rhinovirus illnesses have a greatly increased likelihood of developing childhood asthma. In mice, overexpression of the 17q21 gene ORMDL3 leads to airway remodeling and hyper-responsiveness. However, the mechanisms by which ORMDL3 predisposes to asthma are unclear. Previous studies have suggested that ORMDL3 induces endoplasmic reticulum (ER) stress and production of the type I interferon (IFN) regulated chemokine CXCL10.

Objective

The purpose of this study was to determine the relationship between ORMDL3 and rhinovirus-induced ER stress and type I IFN in human leukocytes.

Methods

ER stress was monitored by measuring HSPA5, CHOP and spliced XBP1 gene expression, and type I IFN by measuring IFNB1 (IFN-β) and CXCL10 expression in human cell lines and primary leukocytes following treatment with rhinovirus. Requirements for cell contact and specific cell type in ORMDL3 induction were examined by transwell assay and depletion experiments, respectively. Finally, the effects of 17q21 genotype on the expression of ORMDL3, IFNB1, and ER stress genes were assessed.

Results

THP-1 monocytes overexpressing ORMDL3 responded to rhinovirus with increased IFNB1 and HSPA5. Rhinovirus-induced ORMDL3 expression in primary leukocytes required cell-cell contact, and induction was abrogated by plasmacytoid dendritic cell depletion. The degree of rhinovirus induced ORMDL3, HSPA5, and IFNB1 expression varied by leukocyte type and 17q21 genotype, with the highest expression of these genes in the asthma-associated genotype.

Conclusions & Clinical Relevance

Multiple lines of evidence support an association between higher ORMDL3 and increased rhinovirus-induced HSPA5 and type I IFN gene expression. These associations with ORMDL3 are cell-type specific, with the most significant 17q21 genotype effects on ORMDL3 expression and HSPA5 induction evident in B cells. Together, these findings have implications for how the interaction of increased ORMDL3 and rhinovirus may predispose to asthma.

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Preventing EGFR Inhibitor-associated Rash

Cutaneous adverse events are common in patients taking EGFR inhibitors. Might tetracyclines offer prophylactic or symptomatic treatment?
The British Journal of Dermatology

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Laser May Be Best Skin Primer for Photodynamic Therapy

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Laser pretreatment seems the most effective way to enhance methyl aminolevulinate hydrochloride-induced protoporphyrin IX (PPIX) formation in photodynamic therapy (PDT), according to Danish researchers.
Reuters Health Information

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Metformin Alleviates Aging Cellular Phenotypes in Hutchinson-Gilford Progeria Syndrome Dermal Fibroblasts

Abstract

Metformin is a popular antidiabetic biguanide, which has been considered as a candidate drug for cancer treatment and aging prevention. Hutchinson-Gilford progeria syndrome (HGPS) is a devastating disease characterized by premature aging and severe age-associated complications leading to death. The effects of metformin on HGPS dermal fibroblasts remain largely undefined. In this study, we investigated whether metformin could exert a beneficial effect on nuclear abnormalities and delay senescence in fibroblasts derived from HGPS patients. Metformin treatment partially restored normal nuclear phenotypes, delayed senescence, activated the phosphorylation of AMP-activated protein kinase, and decreased reactive oxygen species formation in HGPS dermal fibroblasts. Interestingly, metformin reduced the number of phosphorylated histone variant H2AX-positive DNA damage foci and suppressed progerin protein expression, compared to the control. Furthermore, metformin-supplemented aged mice showed higher splenocyte proliferation and mRNA expression of the antioxidant enzyme, superoxide dismutase 2 than the control mice. Collectively, our results show that metformin treatment alleviates the nuclear defects and premature aging phenotypes in HGPS fibroblasts. Thus, metformin can be considered a promising therapeutic approach for life extension in HGPS.

This article is protected by copyright. All rights reserved.



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A case report of cavernous sinus thrombosis after trauma

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Publication date: Available online 13 February 2017
Source:International Journal of Pediatric Otorhinolaryngology
Author(s): Karen Y. Choi, Christina J. Yang
Cavernous sinus thrombosis is a rare but well-documented complication of sinus disease, propagated by intracranial spread of infection via valveless veins of the midface, with facial cellulitis as an uncommon source of infection. We present a case of significant intracranial thromboses secondary to nasal dorsal abscess after trauma that was successfully treated with bedside drainage of the abscess in addition to broad-spectrum antibiotics, anticoagulation, and steroids, and remains asymptomatic with seven months follow-up.



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Systematic reviews and meta-analyses on psoriasis: role of funding sources, conflict of interest, and bibliometric indices as predictors of methodological quality

Summary

Background

The quality of systematic reviews (SRs) and meta-analyses about psoriasis, a chronic inflammatory skin disease that severely impairs quality of life and is associated with high costs, remains unknown.

Objectives

To assess the methodological quality of SRs published on psoriasis.

Methods

After a comprehensive search in MEDLINE, EMBASE, and the Cochrane Database (PROSPERO:CDR4201604161), the quality was assessed by two raters using Assessment of Multiple Systematic Reviews (AMSTAR) tool. Article metadata and journal-related bibliometric indices were also obtained. SRs were classified as low (0-4), moderate (5-8), or high (9-11) quality. A prediction model for methodological quality was fitted using principal component and multivariate ordinal logistic regression analyses.

Results

We classified 220 studies as high (17.2%), moderate (55%), or low (27.7%) quality. Lower compliance rates were found for question (Q) 5 (list of studies provided, 11.3%), Q10 (publication bias assessed, 27.8%), Q4 (status of publication included, 39.5%), and Q1 (a priori design provided, 41%) AMSTAR items. Factors such as meta-analysis including (odds ratio [OR], 6.21; 95% confidence interval [CI]: 2.78-14.85), funding by academic institutions (OR, 2.89; 95% CI, 1.11-7.89), article influence score (OR, 2.13; 95% CI, 1.05-6.67), 5-year impact factor (OR, 1.34; 95% CI, 1.02-1.14), and article page count (OR, 1.08; 95% CI, 1.02-1.15) significantly predicted a higher quality; a high number of authors with a conflict of interest (OR, 0.9; 95% CI, 0.824-0.985) was significantly associated with a lower quality.

Conclusions

The methodological quality of SRs published about psoriasis remains suboptimal. The type of funding sources and author conflicts may compromise study quality, increasing the risk of bias.

This article is protected by copyright. All rights reserved.



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Does drug-induced sleep endoscopy change the surgical decision in surgically naïve non-syndromic children with snoring/sleep disordered breathing from the standard adenotonsillectomy? A retrospective cohort study

Abstract

Background

Adenotonsillectomy is the most commonly performed operation for pediatric snoring/sleep disordered breathing (S/SDB). However, 20–40% of patients will fail to improve. Drug-induced sleep endoscopy (DISE) may provide a more individualized surgical plan and limit unsuccessful surgeries. The aim of this study was to assess the impact of DISE on surgical decision-making in surgically naïve children with S/SDB.

Methods

A retrospective observational cohort study was undertaken at the Stollery Children's Hospital. Patients 3–17 years of age who underwent DISE-directed surgery for S/SDB between January 2009 and December 2015 were eligible. We excluded other indications for tonsillectomy and syndromic children. The primary outcome was the level of agreement between a DISE-based surgical decision and the reference standard based on the American Academy of Pediatrics (AAP) guidelines via un-weighted Cohen's kappa. Secondary outcomes included the frequency and type of alternate surgical targets identified by DISE. The agreement on tonsil size between in-office physical assessment and DISE was also calculated. The effectiveness of DISE-directed surgery on postoperative S/SDB was not investigated.

Results

Five hundred fifty-eight patients were included. DISE changed the surgical plan in 35% of patients. Agreement between DISE-based and AAP clinical practice guidelines-based management was low (κ = 0.354 +/- 0.021 [95% CI 0.312–0.395]). An alternate diagnosis or surgical target was identified by DISE in 54% of patients. There was moderate agreement on tonsil size (κ = 0.44 [0.33–0.55]) between DISE and in-office clinical assessment.

Conclusions

This is a first phase diagnostic study, which demonstrates that DISE affects decision-making in surgically naïve children with S/SDB in up to 35% of patients. It has utility in individualizing first stage surgical treatments as well as identifying alternate targets for further surgical or medical therapy, while potentially limiting unsuccessful surgeries. Further studies to examine the effect of DISE on surgical outcomes are required.



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Formal mentorship in a surgical residency training program: a prospective interventional study

Abstract

Background

Otolaryngology-Head and Neck surgery resident physicians (OHNSR) have a high prevalence of burnout, job dissatisfaction and stress as shown within the literature. Formal mentorship programs (FMP) have a proven track record of enhancing professional development and academic success. More importantly FMP have an overall positive impact on residents and assist in improving job satisfaction. The purpose of the study is to determine the effects of a FMP on the well-being of OHNSR.

Methods

A FMP was established and all OHNSR participation was voluntary. Eight OHNSR participated in the program. Perceived Stress Survey (PSS) and the Maslach Burnout Inventory (MBI) were administered at baseline and then at 3, 6, 9, and 12 month intervals. World Health Quality of Life-Bref Questionnaire (WH-QOL) was administered at baseline and at 12 months.

Results

Baseline statistics found a significant burden of stress and burnout with an average PSS of 18.5 with a high MBI of 47.6, 50.6, and 16.5 for the emotional, depersonalization, and personal achievement domains respectively. Quality of life was also found to be low with a WH-QOL score of 71.9. After implementation of the FMP, PSS was reduced to 14.5 at 3 months (p = 0.174) and a statistically significant lower value of 7.9 at 12 months (p = 0.001). Participants were also found to have lower emotional scores (14.9, p < 0.0001), levels of depersonalization (20.1, p < 0.0001), and higher personal achievement (42.5, p < 0.0001) on MBI testing at 12 months. Overall quality values using the WH-QOL was also found to be significantly improved (37.5, P = 0.003) with statistically significant lower scores for the physical health (33.9, p = 0.003), psychological (41.1, p = 0.001), social relationship (46.9, p = 0.019), and environment (53.5, p = 0.012) domains.

Conclusion

This is the first study to show that FMP can potentially alleviate high levels of stress and burnout within a surgical residency program and achieve higher levels of personal satisfaction as well as overall quality of life.



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Developing a new diagnostic algorithm for human papilloma virus associated oropharyngeal carcinoma: an investigation of HPV DNA assays

Abstract

Background

Human papilloma virus (HPV) has been implicated in the development of a large proportion of oropharyngeal squamous cell carcinoma (OPSCC). Current techniques used to diagnose HPV etiology require histopathologic analysis. We aim to investigate the diagnostic accuracy of a new application non-histopathologic diagnostic tests to help assist diagnosis of HPV-related oropharyngeal tumors.

Methods

Patients with OPSCC with nodal metastasis were consecutively recruited from a multidisciplinary cancer clinic. Appropriate samples were collected and analyzed. The various tests examined included COBAS® 4800, Cervista® HR and Genotyping. These tests were compared to p16 staining, which was used as the diagnostic standard. StataIC 14.2 was used to perform analysis, including sensitivity, specificity and receiver operator characteristic [ROC] curves.

Results

The COBAS® FNA (area under ROC 0.863) and saliva (area under ROC 0.847) samples performed well in diagnosing HPV positive and negative tumors. Samples tested with Cervista® did not corroborate p16 status reliably. We were able to increase the diagnostic yield of the COBAS® FNA samples by applying the results of the saliva test to negative FNA samples which correctly identified 11 additional p16 positive tumors (area under ROC 0.915).

Conclusion

Surrogate testing for HPV using alternate methods is feasible and closely predicts the results of standard diagnostic methods. In the future, these could minimize invasive procedures for diagnosing HPV-related oropharyngeal cancer, but also help to diagnose and treat patients with unknown primaries.



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Management of T4a Laryngeal Cancer

Abstract

Purpose of the Review

The aim of this study is to compare the results of surgical and non-surgical approach for T4a laryngeal cancer in term of curative effects, complications, and functional results by means of a systematic review of the most recent literature.

Recent Findings

Despite the recommendations contained in the guidelines, a general rise in non-surgical treatments and declining use of total laryngectomy has occurred during the last decades. This has paralleled some evidence of declining survival for advanced laryngeal cancer in many countries.

Summary

Recent data clearly demonstrate the superiority of total laryngectomy and post-operative radiotherapy, which produce a substantial long-term control and survival rates for patients with T4 laryngeal cancer. The most negative prognostic factor is represented by the N status. Open partial laryngectomy, in light of its good and encouraging oncological/functional results, could represent an alternative to total laryngectomy for selected patients affected by laryngeal cancer in certain T4a subcategories, i.e., those with limited anterior extension. Considering the worse oncologic results, principally in patients with poor general conditions and T4a cancer, non-surgical larynx preservation should only be addressed to carefully selected patients who refused total laryngectomy, characterized by more limited T4a disease, minimal involvement through the cartilage, and without pre-treatment impairment of laryngeal functions.



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Clinical scenario: A patient with a possible airway obstruction

Engine 21, respond Priority 1 to 226 South Main St. at the Sunrise Café. Two separate callers report a man choking.

You walk into the restaurant and bystanders direct you to a booth in the corner. Your patient is sitting on the floor propped up against the bench with several people standing around.

A woman introduces herself as an off-duty nurse and states that the man was eating alone and abruptly stood up. Another bystander asked if he was choking and then performed the Heimlich maneuver. The patient's eyes are open and he tracks you as you kneel next to him.

You introduce yourself and ask the patient's name. "Matthew," he responds. When asked what happened, Matthew is able to explain the incident and does not appear to be in any distress, other than a slight cough.

As you move through the remainder of your primary assessment, think about the following questions:

  • What is your initial impression of Matthew's presentation?
  • How can the rest of your crew assist at this time?
  • What are the next steps of your primary assessment?

Post your answers in the comments and view the solution for this scenario.



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5 primary assessment tips for EMS providers

The primary assessment as taught to EMS students generally involves some combination of the ABC's, level of consciousness, a general impression of the patient's condition and a definition of treatment priorities for the call. This assessment still plays an important role in determining the flow of a call from start to finish.

The stepwise method taught in school is a great way to remember each phase of the assessment, but can take too long to complete on critical patients. While the primary assessment is taught — and tested — in a linear fashion, there are several ways that a provider can streamline the process. While in school you are required to perform many skills on your own, but practicing medicine as an EMS professional means that you have one or more additional providers at your disposal and you can expedite the process.

1. Multi-task ABC assessments

One reason why EMS students are required to perform each step of a task independently is that instructors and evaluators need a way to ensure that the student actually knows each of the steps involved. Once practicing on her own, however, an EMT or paramedic can find ways to combine steps to speed up the process.

Take the ABC's for example: when assessing a patient's airway, breathing and circulatory status you could certainly perform each evaluation one at a time. Consider, however, walking up, gently taking the patient's wrist to check for a pulse and asking his name. As soon as the patient speaks, you know he has a patent airway since air cannot effectively move past the vocal cords through an occluded airway.

The number of words spoken in a single breath and how the patient's voice sounds can give you some insight into his respiratory status. A patient wheezing and speaking two to three words per breath is struggling while a patient with a clear voice and speaking eight to 10 words per breath is breathing adequately. Also, you should have a general sense for presence and quality of pulse by now. Instead of taking the time to perform each step of the ABC's, you've completed them all at once.

2. Prioritize and delegate

Delegate certain assessment tasks to other EMS providers on scene. In the case of the choking patient, the primary provider may ask his partner to obtain a set of vital signs while she assesses the patient's ABC's. It isn't uncommon to see five or six EMS personnel standing around with only one actually performing any assessment or patient care. As with most decisions in EMS, pick the task that is most important to complete and assign that task first. In my experience, fire department first responders are often great recipients of task delegation because of their experience with chain of command on the fireground.

3. Correct and move one

Often taught alongside the ABC's is the idea that there are certain treatment interventions that should be performed during the primary assessment; generally limited to treatments which are considered lifesaving. While the goal is to complete the primary assessment quickly, it is important for the new EMS provider not to lose sight of the treatments which can and should occur during the primary assessment.

For instance, a patient without a patent airway should have that condition corrected immediately. Start with the least invasive approach or, better yet, delegate that activity and move on to your next assessment step. As a general rule, you should not move on to the next step (breathing) until the current step (airway) is corrected in a satisfactory fashion or has been assigned to someone. There may be calls where you spend your entire time with the patient attempting to manage airway and breathing and don't have a chance to move on to anything else.

Treatments performed during the primary assessment may include: airway maneuvers or adjuncts, assisted ventilations, chest compressions and bleeding control.

With the recent press coverage of EMS and law enforcement treatment of narcotic overdoses and the broadening of MFR and EMT scope of practice in many states to include naloxone, it may be tempting to consider correcting apparent causes of altered mental status during the primary assessment. While this thinking is not entirely incorrect, consider how that method allows other treatment priorities to be missed early in the call. In the case of hypoglycemia, fully understanding the scope of the patient's presentation requires obtaining a blood glucose measurement. For a suspected narcotics overdose, naloxone must be taken out of its storage area, assembled and administered. Both of these activities require multiple steps during which time a patient altered enough to not respond to EMS is also without a patient airway and potentially without adequate respiratory drive.

Instead, consider immediately identifying an altered mental status significant enough to compromise the patient's airway and reposition. Identify inadequate ventilation and assist with a BVM. This serves two purposes: the patient is not left in a hypoxic state unnecessarily and you can continue the primary assessment the same way on every call, correctable causes or not. Remember that practice makes perfect.

4. Don't get distracted

Your first several years in EMS are filled with new experiences. Many calls present some different aspect of assessment and patient care that you've never experienced before. It is easy to get caught up in the excitement and lose track of where you are in your assessment.

Sometimes, certain aspects of a patient's presentation may appear to be pertinent to the underlying condition, but wind up being trivial. Realizing that the primary assessment is geared toward establishing a general sense of the patient's condition it is important to remain focused on completing the assessment before treating the condition or presentation that demands the most attention. With the exception of lifesaving treatments mentioned above, the primary assessment should be completed without stopping. Newer providers may find themselves distracted by significant-looking orthopedic injuries that are far less severe than a closed head injury or significant chest trauma that may not be immediately obvious.

5. Big or little sick?

Since the guiding principle of the primary assessment is to characterize a patient's general condition, it is beneficial to think of their presentation in terms of "big sick" or "little sick." Many of the downstream decisions you will make on a call change based on the classification of the patient's illness. For instance, a "big sick" patient may need a shorter scene time, additional resources, a hospital pre-alert or even air medical transport. By contrast, a "little sick" patient could receive a more thorough evaluation on scene and be transported by a BLS ambulance to a less-specialized hospital.

The question of how sick a patient is does simplify a patient's presentation and sets the tone for the call. The patient priority or severity can always be updated as further assessment findings become available.

Case resolution

Immediately upon contacting Matthew, you can tell that he has a patent airway (he is able to speak) and appears to be breathing adequately. His pulse is strong and regular and you delegate a set of vital signs and a pulse oximetry reading to another responder. Your general impression of Matthew is that he is stable ― little sick ― at this time and that you can remain on scene to evaluate him further. As your assessment progresses, it becomes apparent that Matthew is not experiencing any immediate risk and that the quick thinking of the bystanders cleared his airway and prevented a much worse outcome. Once the ALS unit arrives and you provide a report, Matthew says that he has recovered and ultimately signs a refusal for transport. 



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Lobular capillary hemangiomas: Case report and review of literature of vascular lesions of the nasal cavity

Publication date: Available online 13 February 2017
Source:American Journal of Otolaryngology
Author(s): Akina Tamaki, Eric Babajanian, Brian D'Anza, Kenneth Rodriguez
Vascular tumors of the nasal cavity can represent a variety of pathologies. In this case report, we discuss two patients presenting with a large vascular lesion occupying the nasal cavity. Significant bleeding was encountered during the initial attempts for endoscopic surgical resection. One lesion was successfully excised following preoperative embolization while a second following sphenopalatine artery ligation vascular ligation. In both cases, final pathology showed lobular capillary hemangioma (LCH). We present a literature review and discussion of LCH and other vascular tumors that present in the nasal cavity. In addition, we discuss the utility of pre-resection vascular control of these tumors.



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Is histopathological diagnosis useful in choosing treatments for tumors of the temporal bone?

Publication date: Available online 13 February 2017
Source:American Journal of Otolaryngology
Author(s): Jerzy Kuczkowski, Wojciech Brzoznowski, Jolanta Szade




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Effect of Vitamin D Deficiency on the laryngo-pharyngeal Tract

Publication date: Available online 13 February 2017
Source:American Journal of Otolaryngology
Author(s): Hamdan AL, Ghina Fakhri, Ghassan Haddad, Gabriel Dunya, Doja Sarieddine, Bilal Turfe, George Tabet, Sami Azar
ObjectiveTo investigate the effect of vitamin D deficiency on the laryngopharyngeal tract.Study DesignProspective cohort study.SettingTertiary care center.Subjects and MethodsA total of 38 human subjects were prospectively recruited, equally divided into two groups. The Vitamin D deficiency group defined as 25-OH <25ng/dl and the control subjects with normal vitamin D level defined as 25-OH>25ng/dl. The presence and severity of vocal tract symptoms was assessed using the Vocal Tract Discomfort score.ResultsThere was no significant difference in vocal tract discomfort score for frequency and severity between patients with Vitamin D deficiency and patients with no vitamin D deficiency (p value 0.272). However there was a significant difference in the mean frequency of burning, aching, soreness and lump sensation (p value <0.05) in patients with vitamin D deficiency compared to those with no vitamin D deficiency. There was also a significant difference in the means of vocal tract severity symptoms, namely for burning and aching between patients with vitamin D deficiency compared to patients with no vitamin D deficiency (p value <0.05).ConclusionSubjects with vitamin D deficiency do not have a higher vocal tract discomfort score than subjects with no vitamin D deficiency. However the frequency and severity of certain vocal tract discomfort symptoms was higher and can be based hypothetically on the similarity in structure between the laryngopharyngeal complex and the musculoskeletal system.



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Smoking and pre-existing organ damage reduce the efficacy of belimumab in systemic lupus erythematosus

Publication date: Available online 13 February 2017
Source:Autoimmunity Reviews
Author(s): Ioannis Parodis, Christopher Sjöwall, Andreas Jönsen, Daniel Ramsköld, Agneta Zickert, Martina Frodlund, Azita Sohrabian, Laurent Arnaud, Johan Rönnelid, Vivianne Malmström, Anders A. Bengtsson, Iva Gunnarsson
ObjectivesBelimumab is the first biologic drug approved for Systemic Lupus Erythematosus (SLE). Here, we aimed to investigate the effects of belimumab on clinical and serologic outcomes, and sought to identify predictors of treatment response in three Swedish real-life settings.MethodsFifty-eight patients were enrolled at initiation of belimumab and followed longitudinally for up to 53months. Surveillance outcomes included the SLE Disease Activity Index 2000 (SLEDAI-2K), 100mm Visual Analogue Scales for Physician"s Global Assessment (PGA), fatigue, pain and general health, and the Systemic Lupus International Collaborating Clinics/American College of Rheumatology Damage Index (SDI). Assessment of treatment response included the SLE responder index (SRI). B lymphocyte stimulator (BLyS) levels were determined using ELISA.ResultsSLEDAI-2K (median baseline score: 8.0; IQR: 4.0–13.8), PGA and corticosteroid use decreased during therapy, and patients reported improvements on fatigue, pain, and general health (p<0.0001 for all). SDI scores remained stable (p=0.08). Patients with baseline SDI scores >1 showed decreased probability and prolonged time to attain SRI response (HR: 0.449; 95% CI: 0.208–0.967), as did current smokers compared with non-smokers (HR: 0.103; 95% CI: 0.025–0.427). In contrast, baseline BLyS levels ≥1.2ng/mL predicted increased probability and shorter time to attain SRI response (HR: 2.566; 95% CI: 1.222–5.387).ConclusionsDisease activity and corticosteroid usage decreased, patient-reported outcomes improved, and no significant organ damage was accrued during follow-up. Smoking and organ damage predicted reduced treatment efficacy. These findings might contribute to a better selection of patients who are likely to benefit from belimumab.



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Antiphospholipid antibodies disappearance in primary antiphospholipid syndrome: Thrombosis recurrence

Publication date: Available online 13 February 2017
Source:Autoimmunity Reviews
Author(s): Gabriela Medina García, Eduardo Briones-García, María del Pilar Cruz-Domínguez, Oscar Iván Flórez-Durante, Luis J. Jara
ObjectiveTo evaluate the clinical outcome after aPL (antiphospholipid antibodies) disappearance in primary APS patients.MethodsFrom a cohort of 70 patients with primary APS, we selected patients with positive aPL determinations at onset and ≥2 subsequent negative aPL determinations during the last 5years. To corroborate the immunologic profile, we determined IgG/IgM aCL antibodies, IgG/IgM antiβ2GPl, anti-annexin A5 antibodies and lupus anticoagulant (LA). All patients continued treatment with oral anticoagulants. Clinical data and aPL determinations at onset/after disappearance were obtained. Statistical analysis: descriptive statistics and Kaplan-Meier analysis.ResultsWe found 24 patients with persistently negative aPL, including the last immunologic profile, 17 females, 7 males, mean age 51.7, disease evolution 16.3years, mean of 4 aPL previous positive determinations. aCL was positive at onset in 87.5%, 29% had double aPL positivity at onset (aCL/LA). Deep venous thrombosis (DVT) and ischemic stroke in 33% and pulmonary embolism in 12.5% were the most frequent manifestations at onset. INR range: 2–3. Time with aPL positive 109.4±80.7months. After 60months of follow-up since aPL disappearance, 45.8% of patients presented thrombosis recurrence, DVT in 9 patients, ischemic stroke in 1, pulmonary artery hypertension in 1. Other non-thrombotic APS manifestations were chronic ulcers in lower extremities and severe thrombocytopenia.ConclusionsThis study suggest, that in primary APS, persistent negative aPL profile is not an indication to interrupt oral anticoagulant therapy. However, there is a subset of patients that remained asymptomatic. Other studies are necessary in order to elucidate this controversy.



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Intravenous immunoglobulins in systemic sclerosis: Data from a French nationwide cohort of 46 patients and review of the literature

Publication date: Available online 13 February 2017
Source:Autoimmunity Reviews
Author(s): Sébastien Sanges, Sébastien Rivière, Arsène Mekinian, Thierry Martin, Alain Le Quellec, Emmanuel Chatelus, Alain Lescoat, Patrick Jego, Claire Cazalets, Thomas Quéméneur, Noémie Le Gouellec, Patricia Senet, Camille Francès, Alban Deroux, Bernard Imbert, Olivier Fain, Latifatou Boukari, Thomas Sené, Christophe Deligny, Alexis Mathian, Christian Agard, Grégory Pugnet, Silvia Speca, Sylvain Dubucquoi, Pierre-Yves Hatron, Éric Hachulla, David Launay
BackgroundAs intravenous immunoglobulins (IVIG) exhibit immunomodulatory and antifibrotic properties, they may be a relevant treatment for systemic sclerosis (SSc). The objectives of this work were thus to report on the efficacy and safety of IVIG in a population of SSc patients and to review the available literature.Methods46 patients from 19 French centers were retrospectively recruited. They were included if they had a diagnosis of SSc and received at least 1 IVIG infusion at a dosage >1g/kg/cycle. Relevant data collected at IVIG discontinuation were compared to those collected at IVIG initiation. A comprehensive literature review was performed.ResultsWe observed a significant improvement of muscle pain (74% vs. 20%, p<0.0001), muscle weakness (45% vs. 21%, p=0.01), joint pain (44% vs. 19%, p=0.02), CK levels (1069±1552 UI vs. 288±449 UI, p<0.0001) and CRP levels (13.1±17.6mg/L vs. 9.2±16.6mg/L, p=0.001). We also noted a trend for an improvement of gastro-esophageal reflux disease (68% vs. 53%, p=0.06) and bowel symptoms (42% vs. 27%, p=0.06). Skin and cardiorespiratory involvements remained stable. Finally, corticosteroid daily dose was significantly lower by the end of treatment (13.0±11.6mg/d vs. 8.9±10.4mg/d, p=0.01). Only two severe adverse events were reported (one case of deep vein thrombosis and one case of diffuse edematous syndrome).ConclusionOur work suggests that IVIG are a safe therapeutic option that may be effective in improving musculoskeletal involvement, systemic inflammation, digestive tract symptoms and could be corticosteroid sparing.



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Autoimmune Neuropathies associated to Rheumatic Diseases

Publication date: Available online 12 February 2017
Source:Autoimmunity Reviews
Author(s): Alberto R.M. Martinez, Ingrid Faber, Anamarli Nucci, Simone Appenzeller, Marcondes C. França
Systemic manifestations are frequent in autoimmune rheumatic diseases and include peripheral nervous system damage. Neuron cell body, axons and myelin sheath may all affected in this context. This involvement results in severe and sometimes disabling symptoms. Sensory, motor and autonomic features may be present in different patterns that emerge as peculiar clinical pictures. Prompt recognition of these neuropathies is pivotal to guide treatment and reduce the risks of long term disability. In this review, we aim to describe the main immune-mediated neuropathies associated to rheumatic diseases: sensory neuronopathies, multiple mononeuropathies and chronic inflammatory demyelinating polyradiculoneuropathy, with an emphasis on clinical features and therapeutic options.



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Autoimmunity in Guillain-Barré syndrome associated with Zika virus infection and beyond

Publication date: Available online 13 February 2017
Source:Autoimmunity Reviews
Author(s): Carlos A. Pinto-Díaz, Yhojan Rodríguez, Diana M. Monsalve, Yeny Acosta-Ampudia, Nicolás Molano-González, Juan-Manuel Anaya, Carolina Ramírez-Santana
Autoimmune diseases share common immunopathogenic mechanisms (i.e., the autoimmune tautology), which explain the clinical similarities among them as well as their familial clustering. Guillain-Barré syndrome (GBS), an autoimmune peripheral neuropathy, has been recently associated with Zika virus (ZIKV) infection. Based on a series of cases, this review article provides a comparative analysis of GBS associated with ZIKV infection, contrasted with the general characteristics of GBS in light of the autoimmune tautology, including gender differences in prevalence, subphenotypes, polyautoimmunity, familial autoimmunity, age at onset, pathophysiology, ecology, genetics, ancestry, and treatment.



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Ultrastructural Characterization of Mammary Analogue Secretory Carcinoma of the Salivary Glands: A Distinct Entity from Acinic Cell Carcinoma?

Abstract

Mammary analogue secretory carcinoma (MASC) of the salivary glands is a recently described neoplasm of the salivary glands with a characteristic morphology complemented by a specific cytogenetic translocation and gene rearrangements. Although immunophenotypic and cytogenetic differences allow for a more reliable distinction, ultrastructural features can also provide important information about the relationship between MASC, classic acinic cell carcinoma (AciCC), and AciCC intercalated duct cell-predominant variant. Following approval from the hospital's institutional review board, 7 cases of MASC, 8 cases of classic AciCC, and 4 cases of AciCC intercalated duct cell-predominant variant were retrieved from the pathology files of Massachusetts General Hospital from 2012 to 2015. Electron microscopy was performed using formalin-fixed, paraffin-embedded tissue. Ultrastructural features of all 19 neoplasms of the salivary glands were recorded. The predominant cell-types observed in MASC are those with intercalated/striated duct cell differentiation. These features include prominent invaginations of the cell surface studded with microvilli, and some intra- and intercellular lumina also with a microvillous surface. Classic AciCC dominant cell-type recapitulates acinar cell differentiation. These cells contain large intracytoplasmic zymogen-like granules. AciCC intercalated duct cell-predominant variant showed both cell populations in various proportions with the intercalated/striated duct cell type usually being the dominant one. MASC presents with distinctive ultrastructural features that allows its proper differentiation from classic AciCC. However, significant ultrastructural features overlaps between both AciCC intercalated duct cells-predominant and classic AciCC and MASC. These findings indicate a very close proximity between these tumors.



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Calcium Electroporation for Head and Neck Cancer

Condition:   Head Neck Cancer
Interventions:   Drug: Calcium chloride;   Device: Electroporation
Sponsors:   Rigshospitalet, Denmark;   Herlev Hospital
Recruiting - verified February 2017

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Reproducibility Study of Transcranial Doppler

Condition:   Stroke
Intervention:   Diagnostic Test: Transcranial Doppler Ultrasound
Sponsor:   University of Edinburgh
Not yet recruiting - verified February 2017

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Associated Injuries in Maxillofacial Trauma: A Study in a Tertiary Hospital in South India

Abstract

Aim

Maxillofacial trauma when associated with concomitant injuries has a significant potential for increased morbidity. This study aims to identify the causes of trauma, evaluate the types of associated injuries and to highlight the significance of multi professional collaboration in sequencing of treatment.

Patients and Methods

A total of 300 patients who reported to the casualty of a tertiary Hospital in Karnataka with facial fractures were enrolled.

Results

Associated injuries were sustained by 162 patients. The predominant aetiology was the Road Traffic Accident with maximum number of patients in the age group of 20–29 and a male to female ratio of 10.1:1. The mandible was the most frequently fractured bone. Head injury was the most common associated injury. The mortality rate was 0.66%. The mean ISS and GCS values among the patients who sustained associated injuries along with maxillofacial trauma were higher and lower respectively, as compared to those without associated injuries with a statistically significant difference (p < 0.001).

Conclusion

Implementation of strict road safety measures in the rural and interior regions of South India, to prevent morbidity and mortality due to road traffic accidents is essential. Injuries to the facial skeleton must be approached with the knowledge of probable associated injuries that could have been incurred.



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Basal cell carcinoma arising in a congenital melanocytic naevus in an adult

Congenital melanocytic naevi (CMN) are common skin lesions. They harbour a risk of malignant transformation, and various lesions have been described as developing within them. A basal cell cancer occurring within a CMN has never previously been described. A case is described of a woman aged 52 years presenting with a slow-growing, symptomatic 3 cm lesion in the centre of a 10x5 cm CMN on her right upper back. Diagnostic core biopsy revealed an ulcerated, infiltrative basal cell carcinoma which was then further excised. The scar has healed with no evidence of local recurrence at 1-year follow-up.



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An unusual vaginal lump

A 46-year-old woman presented with a 2-week history of a painless golf ball sized lump in her vagina. Despite transvaginal ultrasound scan, biopsy and MRI, it was only after surgery had been performed that it became clear what the nature of the mass actually was.

Immunohistochemistry revealed the lump to be an aggressive angiomyxoma. Follow-up MRI showed residual (or recurrence of) angiomyxoma which was successfully treated with monthly injections of Prostap (leuprorelin acetate), a gonadotropin-releasing hormone agonist. Further MRI showed complete resolution of the lesion.



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Tumour thrombus of the inferior vena cava extending into the right atrium in the setting of colon cancer

Tumour thrombus is seen in renal cell and hepatocellular carcinoma, but is rarely reported in colorectal cancer. A woman aged 46 years, with metastatic colonic adenocarcinoma, was found to have a large mass in the inferior vena cava (IVC) extending into the right atrium. Although this lesion was initially thought to be bland thrombus, imaging with contrast-enhanced CT scan and contrast-enhanced ultrasound supported the diagnosis of tumour thrombus. Despite the large size of the lesion, the patient was asymptomatic. Her lack of symptoms, and poor overall prognosis from her cancer led to the decision to avoid aggressive surgical or radiological interventions. Several months later, the patient passed away. At autopsy, there was no evidence of fatal embolisation from the pre-existing thrombus. Diagnosis of tumour thrombus in the IVC is difficult and management can be challenging due to the significant risks associated with treatment options.



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Not your usual constipation: stercoral perforation

Stercoral perforation is a rare cause of bowel perforation. It is caused by faecal impaction, which generates pressure against the colon wall, leading to ischaemic necrosis and subsequent perforation. Since diagnosis is often delayed, stercoral perforation is usually mistreated as constipation or faecal impaction, leading to high mortality. This report presents a case of stercoral perforation in a woman aged 34 years who was promptly diagnosed and successfully treated.



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Erratum to ‘Cytoreductive surgery for head and neck squamous cell carcinoma in the new age of immunotherapy’ [Oral Oncol. 61 (2016) 166–176]

Publication date: Available online 12 February 2017
Source:Oral Oncology
Author(s): R. Bryan Bell, Michael J. Gough, Steven K. Seung, Zeljka Jutric, Andrew D. Weinberg, Bernard A. Fox, Marka R. Crittenden, Rom S. Leidner, Brendan Curti




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Title Page



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Editorial Board



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Table of Contents



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Acute localised exanthematous pustulosis: What are the diagnostic criteria?



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Generalised pustular psoriasis and neutrophilic cholangitis: An infrequently reported association with excellent response to tumour necrosis factor inhibitors



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Long term remission of persistent adult acne following very low-dose (5 mg/day) isotretinoin



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Phymatous rosacea presenting with leonine facies and clinical response to isotretinoin



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Melanosis of the lower lip subverted by filler injection: a simulator of early mucosal melanoma



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Issue Information



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Dr John Brenan



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Drug reaction with eosinophilia and systemic symptoms in metastatic basal cell carcinoma treated with vismodegib



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Surgical Rehabilitation for Anophthalmic Sockets Devoid of Orbital Implant

Publication date: Available online 13 February 2017
Source:Journal of Cranio-Maxillofacial Surgery
Author(s): Doo-Ri Eo, Kyung In Woo, Yoon-Duck Kim
PurposeTo determine the necessity of rehabilitative surgical procedures for no implant anophthalmic sockets, and predictive factors of corrective operations after secondary orbital implantation.Material and methodsNineteen unilateral anophthalmic patients without orbital implant were included. The distance of eyebrow, upper eyelid margin, and lower eyelid margin from the horizontal medial canthal line (BM, UM, and LM, respectively) was measured using photographs. The anophthalmic orbit anatomy was compared with that of the healthy side using CT scans.ResultsFive (26.3%) patients showed satisfactory results with the secondary implantation alone. Fourteen (74%) patients needed additional surgeries for ptosis, shallow inferior fornix, enophthalmos, or lower eyelid malposition. Separated superior muscle complex and prominent intermuscular septum connecting the levator and the lateral rectus muscles were noticeable in CT scans. Predictive factors for ptosis surgery included longer BM (p = 0.04), shorter distance from the superior orbital wall to the upper margin of the prosthesis (p < 0.01), and a longer height of the prosthesis (p = 0.04).ConclusionMost patients needed multiple operations after secondary implantation for rehabilitation. Additional ptosis operation may be required for patients with a high brow on the anophthalmic side, a vertically long prosthesis, and an impinged prosthesis against the superior orbital wall.



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Treatment Outcome in Orthognathic Surgery - a prospective comparison of accuracy in computer assisted two and three-dimensional prediction techniques

Publication date: Available online 13 February 2017
Source:Journal of Cranio-Maxillofacial Surgery
Author(s): Martin Bengtsson, Gert Wall, Patricia Miranda-Burgos, Lars Rasmusson
The main objective of the present study was to assess the accuracy of two- and three-dimensional prediction techniques in orthognathic surgery. It was also a test of the very planning sequence. The scientific question was how well does the software support the surgeon in his way to find the perfect correction of the facial appearance while normalizing the occlusion?Thirty patients with a class III occlusion were included in this prospective study. Surgical planning with both techniques were undertaken for all patients. Surgery was performed according to the two-dimensional technique. The cephalometric measurements from two-dimensional and threedimensional predictions were compared with the postoperative results at the 12 months follow-up respectively.Together with an analysis of tracing error, placements of 2020 markers, 1860 measurements and 1280 comparisons was performed. The analysis showed an equally high accuracy for the studied techniques. The highest accuracy was found in the anterior maxilla. There was a tendency for an overestimation for the three-dimensional technique and an underestimation for the two-dimensional technique.ConclusionsThe present study indicates an equal high accuracy in predicting facial outcome for both studied techniques. However, in those patients with asymmetric malocclusion and/or facial appearance the three-dimensional technique has an obvious advantage.



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Comparison of functional outcomes and patient-reported satisfaction between titanium and absorbable plates and screws for fixation of mandibular fractures: a one-year prospective study

Publication date: Available online 13 February 2017
Source:Journal of Cranio-Maxillofacial Surgery
Author(s): Maria Belén Leno, Stanley Yung Liu, Chien-Tzung Chen, Han-Tsung Liao
PurposeThe aim of this study is to compare the 1-year functional outcomes and patient-reported satisfaction in treating mandibular fractures between resorbable and titanium fixation devices.Materials and MethodsA 1-year prospective study was conducted; 41 consecutive patients presenting with mandibular fractures were included. A resorbable system was used in 21 patients, while in 20 patients a titanium fixation device was used. Functional outcome was evaluated objectively at several time points (2, 4 and 6 weeks, 3 and 6 months, and 1 year after surgery). Bite forces over molars and incisors, mouth opening distance, occlusal status, operation time, fee for implants, bone healing and plate-associated complications were evaluated. Functional and overall satisfaction was measured by patients themselves subjectively.ResultsA statistical difference was found only in maximal mouth opening and molar bite force, both greater for the titanium group in the 2-week time point, achieving comparable measurements in subsequent ones. This coincides with the patient-reported statistically lower satisfaction rates. The cost of the resorbable device was nearly 3 times more expensive than the titanium devices.ConclusionResorbable fixation can achieve stability of bone healing at 1 year postoperatively, with functional and satisfaction outcomes comparable to those associated with titanium hardware from the fourth week postoperatively, while yielding unique advantages.



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Retrospective Evaluation of Three Different Joint Surgeries for Internal Derangements of the Temporomandibular Joint

Publication date: Available online 13 February 2017
Source:Journal of Cranio-Maxillofacial Surgery
Author(s): Celal Candirli, Mehmet Demirkol, Onur Yilmaz, Sadi Memis
PurposeTo compare the outcomes achieved with eminectomy, discectomy alone, and discectomy with an abdominal dermis fat graft (D+ADFG) in the treatment of internal derangements (IDs) of the temporomandibular joint (TMJ), and to briefly discuss the failed cases in which a second operation was needed.Patients and MethodsThis was a retrospective study of 31 patients (27 females, 4 males) who were unresponsive to conservative therapy and therefore underwent eminectomy (group A, n = 6), discectomy alone (group B, n = 8), or D+ADFG (group C, n = 17). All of the IDs of the TMJ were unilateral. Functional mandibular pain intensity on a visual analog scale (VAS) and maximum interincisal opening (MIO) were recorded preoperatively and at the latest postoperative examination (average, 30 months). The preoperative and postoperative results were compared between and within each group.ResultsOverall, patients in groups B and C had better outcomes than those in group A in terms of TMJ symptom relief. Postoperative increases in MIO were statistically significant in groups A, B, and C (P < 0.027, P < 0.017, and P <0 .000, respectively). Patients with discectomized joints (groups B and C) had significantly improved pain scores (P < 0.05) at the latest follow-up. In 6 of the 31 (19.3%) patients (3 eminectomy, 1 discectomy, and 2 D+ADFG), the procedure was deemed unsuccessful because of persistent symptoms and a second operation was performed.ConclusionsDiscectomy with or without ADFG was clearly superior to eminectomy in reducing pain and improving joint function. But there were no significant differences in the two-discectomy groups with respect to symptom improvement.



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Radiation-free 3D head shape and volume evaluation after endoscopically assisted strip craniectomy followed by helmet therapy for trigonocephaly

Publication date: Available online 12 February 2017
Source:Journal of Cranio-Maxillofacial Surgery
Author(s): Guido de Jong, Manon Tolhuisen, Jene Meulstee, Ferdi van der Heijden, Erik van Lindert, Wilfred Borstlap, Thomas Maal, Hans Delye
IntroductionRadiation-free 3D post-operative sequential follow-up in craniosynostosis is hindered by the lack of consistent markers restricting evaluation to subjective comparison. However, using the computed cranial focal point (CCFP), it is possible to perform correct sequential image superposition and objective evaluation. We used this technique for mean volume and shape change evaluation of the head utilizing 3D photos after endoscopically assisted trigonocephaly surgery.MethodsWe performed a mean head shape and volume evaluation on age grouped 3D photos (n=86) of children who underwent endoscopically assisted strip craniectomy with helmet therapy. We used CT-scans of healthy children as reference. We performed a mean shape evolution analysis and calculated the anterior fossa to total volume ratio (A/T-ratio). The volume- and A/T-ratio pattern were compared with the reference group.ResultsThe mean anterior fossa volume evolved from 336ml (33.4% A/T-ratio) pre-surgery to 664ml (36.0% A/T-ratio) at 37-48 months post-surgery. Both groups have a near similar volume- and A/T-ratio pattern over time. The first 18 months show a predominant growth around the resected metopic suture. Between 18 and 24 months we observed mostly anterior orbital rim growth. From 24 months till 36-48 months the head grows predominantly at the temporal area. The least outward growth was observed at the temporal bones.ConclusionUsing a novel technique we were able to objectively evaluate head shape and volume using stereophotogrammetry after endoscopically assisted strip craniectomy. The A/T-ratio and volume growth pattern of endoscopically treated patients is near identical to that of the normal reference group.



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Structured evaluation and need-based restructuring of the cranio-maxillofacial surgery module within surgical clerkship

Publication date: Available online 13 February 2017
Source:Journal of Cranio-Maxillofacial Surgery
Author(s): Sebastian Herbert Hoefer, Jasmina Sterz, Bernd Bender, Christina Stefanescu, Marius Theis, Felix Walcher, Robert Sader, Miriam Ruesseler
BackgroundEvaluations are important for teaching courses and contribute to educational quality assurance. CMF surgery provides a module in the skills-lab week in preparation for surgical clerkship. Even though the CMF module receives positive evaluations, the students report deviating content. Subsequently, exams skills were often not mastered correctly. The aim of this study is to gather the contents taught within the course and to revise the module accordingly.MethodsA structured evaluation sheet was used to evaluate the CMF modules. The detailed time frame used, teaching methods integrated, and learning objectives taught were documented. Based on the results, the module was restructured and re-evaluated twice.ResultsThere were substantial fluctuations among the taught learning objectives in the first evaluation (21% to 47% of the objectives were totally covered). The deployed time (160.50±32.55 min) for the module was much shorter than scheduled (210 min).After restructuring, more learning objectives were totally covered (44% to100%), which corresponds to a significant gain (p=.024). The deployed teaching time for the modules was used more efficiently (183.65±21.10 min/p=.005), and the additional time (51.89 ±21.23 min vs. 37.55 ±16.06 min before/p=.011) was used mainly for practical exercises.ConclusionStructured evaluations are a meaningful tool for gaining valuable insights regarding the contents and quality of teaching courses and pinpointing potential for improvement. Key factors for the improvement of an educational module are the definition of learning goals within the context of a transparent and structured module.



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Perioral soft tissue change after isolated mandibular surgery for asymmetry patients

Publication date: Available online 12 February 2017
Source:Journal of Cranio-Maxillofacial Surgery
Author(s): Eun-Gyu Jeon, Sung-Tak Lee, Tae-Geon Kwon
IntroductionThis study was intended to determine whether isolated mandibular surgery for the correction of asymmetry could also change perioral soft tissue asymmetry.Patients and MethodsSkeletal class III patients who had undergone mandibular set-back surgery were included. The subjects were composed of two groups with (n=20) or without (n=30) menton (Me) deviation over 4mm. The perioral lip landmarks were analyzed on three-dimensional image from cone-beam computed tomography taken before and 6 months after the operation. The bilateral and inter-group differences and pre- and post-operative changes were statistically analyzed.ResultsThe corner of mouth on the deviated side was 1.9 mm shorter vertically than that on the contralateral side in patients with asymmetry. After mandibular surgery, the deviated Me moved 5.5 mm to the contralateral side and the lip canting was corrected by 2.4° in the asymmetry group. The degree of Me deviation was significantly correlated with the degree of midline asymmetry in perioral soft tissue landmarks including subnasale, upper and lower lip midline. The predictor variable that affected the changes in lip cant was the surgical correction of Me deviation.DiscussionThe correction of chin deviation by isolated mandibular surgery could significantly improve the subnasal and lip asymmetry.



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Preliminary research on DNA methylation changes during murine palatogenesis induced by TCDD

Publication date: Available online 12 February 2017
Source:Journal of Cranio-Maxillofacial Surgery
Author(s): Chen Wang, Xin-gang Yuan, Cui-ping Liu, Sha-na Zhai, Ding-wen Zhang, Yue-xian Fu
2,3,7,8-tetrachlrodibenzo-p-dioxin (TCDD) has been shown to induce cleft palate through growth factor and receptor expression changes during palatogenesis. DNA methylation is an important epigenetic modification that can regulate gene expressions and may be involved in TCDD-induced cleft palate. In this study, we investigated the effects of TCDD on the global and CpG DNA methylation status and the expression levels of DNA methyltransferases(Dnmts) in palate tissue of fetal mice. Pregnant C57BL/6J mice were administered with corn oil or TCDD 28μg/kg at gestation day 10.5(GD10.5), and sacrificed at GD13.5,14.5,15.5.Fetal palates were collected for molecular analysis. Global DNA methylation status was detected by MethylampTM Global DNA Methylation Quantification Ultra Kit. The expression of DNA methyltransferases were examined by quantitative real-time PCR(q-PCR). Methylation Specific PCR (MSP) was performed to analyze CpG methylation status of Dnmts. We found that the global DNA methylation level and the expression of Dnmt3a were higher at GD13.5 in the TCDD group. The methylation level of CpG site 2 in the promoter region of Dnmt3a in the control group was higher than that of the TCDD group at GD13.5. The low CpG methylation level of Dnmt3a at GD13.5 which causes the up-expression of Dnmt3a may induce global hypermethylation in fetal palate tissue. The aberrant global methylation status at GD13.5 may be the cause of palate malformation in fetal mice induced by TCDD.



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The Current Preferred Female Lip Ratio

Publication date: Available online 12 February 2017
Source:Journal of Cranio-Maxillofacial Surgery
Author(s): Paul I. Heidekrueger, Sabrina Juran, Caroline Szpalski, Lorenz Larcher, Reuben Ng, P. Niclas Broer
BackgroundPerception of beauty is influenced by the individual's demographic background and characteristics. However, objective measurements and ratios remain the foundation for aesthetic evaluations. This study aimed to elucidate if there exists a universally applicable ideal upper to lower lip ratio.MethodsAn interactive online survey was designed. Modifiable ranges of lip ratios were achieved via digital alteration, enabling participants to change the ratio of a single female model's lips. The questionnaire was translated into multiple languages and sent to more than 9,000 plastic surgeons and the general public worldwide. Demographic data were collected and analysis of variance was used to investigate respective lip ratio preferences.ResultsA total of 1,011 responses from 35 different countries (response rate of 14%) was gathered. The majority of survey takers (60 %) chose the 1.0: 1.0 lip ratio as most attractive. No differences were found in respect to lip ratio preference and the self-reported ethnicity. However, interesting preferences prevailed when analyzing the subgroups regarding lower lip size.ConclusionAge, gender, country of residence, and profession significantly impact individual upper to lower lip ratio preferences. However, a 1.0: 1.0 lip ratio can apparently be considered most pleasing in females.



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Radiological changes with magnetic resonance imaging and computed tomography after irradiating minipig mandibles: The role of T2-SPIR mixed signal intensities in the detection of osteoradionecrosis

Publication date: Available online 12 February 2017
Source:Journal of Cranio-Maxillofacial Surgery
Author(s): Lucas J. Poort, Alida A. Postma, Annika A.R. Stadler, Roland A. Böckmann, Frank J. Hoebers, Peter A.W.H. Kessler
PurposeRadiotherapy in the head and neck can induce several radiologically detectable changes in bone, osteoradionecrosis (ORN) among them. The purpose is to investigate radiological changes in mandibular bone after irradiation with various doses with and without surgery and to determine imaging characteristics of radiotherapy and ORN in an animal model.Materials and MethodsSixteen Göttingen minipigs were divided into groups and were irradiated with two fractions with equivalent doses of 0, 25, 50 and 70 Gray. Thirteen weeks after irradiation, left mandibular teeth were removed and dental implants were placed. CT-scans and MR-imaging were made before irradiation and twenty-six weeks after. Alterations in the bony structures were recorded on CT-scan and MR-imaging and scored by two head-neck radiologists.ResultsIncreased signal changes on MR-imaging were associated with higher radiation doses. Two animals developed ORN clinically. Radiologically mixed signal intensities on T2-SPIR were seen. On CT-scans cortical destruction was found in three animals. Based on imaging, three animals were diagnosed with ORN.ConclusionIrradiation of minipig mandibles with various doses induced damages of the mandibular bone. Imaging with CT-scan and MR-imaging showed signal and structural changes that can be interpreted as prolonged and insufficient repair of radiation induced bone damages.



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Resolution of neurosensory deficit after mandibular orthognathic surgery: A prospective longitudinal study

Publication date: Available online 12 February 2017
Source:Journal of Cranio-Maxillofacial Surgery
Author(s): Albraa Badr Alolayan, Yiu Yan Leung




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Bone regeneration enhancement by ultra-violet (UV) treatment for uHA/PLLA absorbable mesh

Publication date: Available online 12 February 2017
Source:Journal of Cranio-Maxillofacial Surgery
Author(s): Hiroumi Ikawa, Akinori Moroi, Kunio Yoshizawa, Yuriko Saida, Asami Hotta, Takamitsu Tsutsui, Kenichi Fukaya, Ryota Hiraide, Akihiro Takayama, Tatsuya Tsunoda, Yuki Saito, Koichiro Ueki
PurposeThe purpose of this study was to evaluate the effect of bone regeneration enhancement by ultra-violet (UV) treatment of an unsintered hydroxyapatite (u-HA)/poly-L-lactic acid (PLLA) mesh in rabbitnasal bone.Materials and methodsThirty adult male Japanese white rabbits (12-16 weeks, 2.5-3.0 kg) were used in this study. After incising along the nasal bone, 6 × 6 × depth 1 mm two bone defects were made on both sides. In the UV group (n = 30), the defects were covered with uHA/PLLA mesh, treated by UV (wavelength 172 nm, tube wall illumination 13 mW/cm2, period 8 min, intensity 6.26J/cm2) and screwed. In the control group (n = 30), untreated uHA/PLLA was applied in a similar manner. The rabbits were sacrificed at 1, 2, 3, 4 and 6 weeks postoperatively, and formalin-fixed specimens were frozen. The specimens were stained with hematoxylin and eosin. For immunohistochemical analysis, the specimens were treated with anti-alkaline phosphatase (ALP). Finally, bone ratio and ALP expression were evaluated microscopically.ResultsThe UV group had a significantly higher number of ALP stained cells than the control group after 1, 2, and 3 weeks (P < 0.05). The bone ratio was also significantly higher in the UV group than in the control group after 1, 2, 3, and 4 weeks (P < 0.05).ConclusionThis study suggests that bone regeneration can be enhanced by UV treatment using an uHA/PLLA mesh.



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Involvement of the phosphoinositide 3-kinase/Akt signaling pathway in bone morphogenetic protein 9-stimulated osteogenic differentiation and stromal cell-derived factor 1 production in human periodontal ligament fibroblasts

Recent studies have shown that bone morphogenetic protein 9 (BMP-9) can induce osteogenic differentiation in human periodontal stem cells and human periodontal ligament fibroblasts (PDLFs). Bone morphogenetic protein 9 may be used in periodontal tissue regeneration because of its potent osteoinductive ability. Human periodontal ligament cells also have been demonstrated to produce stromal cell-derived factor 1 (SDF-1), which is important for stem-cell homing and recruitment to injured sites. In the present study, we examined the involvement of the phosphoinositide 3-kinase (PI3K)/Akt signaling axis in osteogenic differentiation and SDF-1 production in human PDLFs stimulated with BMP-9 in osteogenic medium supplemented with dexamethasone and ascorbic acid. Pretreatment of the cells with LY294002, a PI3K-specific inhibitor, suppressed not only BMP-9-enhanced alkaline phosphatase activity but also expression of a BMP-response gene (inhibitor of DNA binding 1) and osteogenic marker genes (runt-related transcription factor 2, osterix, bone sialoprotein, and osteopontin). In addition, BMP-9 up-regulated SDF-1 production, and the production of SDF-1 was suppressed by LY294002. The protein SDF-1-alpha was identified as a major isoform of SDF-1 that was regulated by BMP-9. Our data suggest involvement of the PI3K/Akt pathway in BMP-9-stimulated osteogenic differentiation and SDF-1 production in PDLFs cultured in osteogenic medium.



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Immediate-type allergic and protease-mediated reactions are involved in scratching behavior induced by topical application of Dermatophagoides farinae extract in NC/Nga mice

Abstract

Atopic dermatitis (AD)-like dermatitis can be induced by repeated topical application of an ointment containing Dermatophagoides farinae body (Dfb) extract in NC/Nga mice. This AD-like murine model also exhibits a biphasic increase in the number of scratching behavior after topical application of Dfb ointment. In this study, we investigated the possible mechanisms underlying the scratching behavior in each phase. An increase in the content of mast cell derived mediators such as histamine and 5-hydroxytryptamine in the lesional skin and increased vascular permeability were observed in the early phase after the Dfb ointment application. Chlorpheniramine (H1 receptor antagonist) and cromoglycate (mast cell stabilizer) reduced the scratching behavior in the early phase but not that in the later phase. Furthermore, the content of various endogenous pruritogens such as interleukin-31 and thymic stromal lymphopoietin in the lesional skin was increased 1 or 24 hours after the Dfb ointment application. Elevated expression of proteinase-activated receptor-2 (PAR-2) was also observed in the epidermis. Finally, gabexate (serine protease inhibitor) reduced the scratching behavior in both phases, and anti-PAR2 antibody also showed a tendency to reduce both scratching behaviors. These findings suggest that immediate-type allergic reactions caused by mast cell degranulation and PAR-2 activation by proteases are involved in the scratching behavior in this AD-like model.

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Mutational profiling of acral melanomas in Korean populations

Abstract

The proportion of acral melanoma (AM) is much higher in Asian populations than in Caucasians populations. Although mutational profiles associated with AM have been discovered in Caucasian populations, knowledge of its genetic alterations in the Asian populations is limited. To describe the molecular nature of AM in Korean patients, we performed mutational profilinge of AM and matched normal tissues of in patients. Fifty-one formalin-fixed paraffin-embedded AM samples and 32 matched pairs of from patients' saliva DNA were analyzed by next-generation sequencing. Only mutations confirmed via digital droplet PCR or in BRAF, KIT and NRAS, the most frequently altered cancer genes in cutaneous melanoma, were considered as positive. The rRelationship between mutational status and clinicopathological features were examined. Of the 47 AM patients screened, alteration of BRAF, NRAS, and KIT genes was observed in 6.4%, 4.3%, and 8.5%, respectively. We also tested matched normal tissues of patients to identify tumor-specific mutations. Examination of the mutational profile in a cohort of 28 primary melanomas and matched normal controls found BRAF mutations in 2 cases (7.1%), KIT mutations in 3 cases (10.7%), and CTNNB1 mutations in 1 case (3.6%). The BRAF, NRAS, and KIT mutation status did not correlate with its clinicopathological characteristics. Our results show that KIT, NRAS, and BRAF hotspot mutations occur at a low frequency in Korean populations. We also observed a case with the CTNNB1 mutation, which raises the possibility that other pathways are associated with AM development.

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Migrated prawn tail presenting as neck swelling

Publication date: Available online 13 February 2017
Source:Egyptian Journal of Ear, Nose, Throat and Allied Sciences
Author(s): Hafizah Husna Johari, Irfan Mohamad, Imran Azmi
Accidental ingestion of food-based foreign body such as fish bones and meat boluses are common among adults. Cases of migrated foreign bodies particularly the sharp-edged ones are also frequently encountered. The presentations of migrated foreign bodies in the neck can initially be asymptomatic and subsequently patients will come with complaint of foreign body or sharp pricking sensation on swallowing, odynophagia, fever or neck swelling. We report a case of a 61-year-old Chinese gentleman who presented with painful left neck swelling with history of eating prawn including its tail 2months prior to presentation. He was symptoms free after eating few boluses of rice since then. With positive findings on imaging studies, the neck lesion was successfully excised under local anesthesia. It contained a migrated intact prawn tail. In conclusion, accidental foreign body ingestion is not uncommon among adults. It is very important to elicit history of foreign body ingestion even in a patient presenting with neck swelling. Besides, oral and neck examination, other investigation modalities such as plain neck radiograph, ultrasonography or computed tomography scan can confirm the diagnosis of migrating foreign bodies and appropriate treatment can be instituted.



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Acquired Hair Curling after Diphenylcyclopropenone Immunotherapy in Alopecia Areata Patient

Abstract

Contact immunotherapy using diphenylcyclopropenone (DPCP) is commonly used treatment modality for moderate to severe alopecia areata (AA)1. The most common side effects associated with topical immunotherapy using DPCP include eczema, hyperpigmentation, hypopigmentation, vitiligo, and cicatricial alopecia.

However, we identified an interesting case of a patient whose entire scalp of straight hair (Figure 1A) became curly after DPCP immunotherapy, which has not been reported previously. A 24-year-old male patient with no specific medical or dermatological history reported to our dermatology outpatient clinic because of sudden hair loss and started treatment after being diagnosed with AA.

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Non-invasive skin imaging for the diagnosis of myiasis

Abstract

A 52-year-old man presented with a painful ulceration of the scalp (Fig. 1a). He had returned from Guyana the previous week. Dermoscopic examination (FotoFinder Systems GmbH, Bad Birnbach, Germany) showed an intermittent dynamic aspect changing from a sanguineous roundish ulcer (Fig. 1b) to a peculiar roundish structure characterized by a yellowish peripheral ring and a central brownish part (Fig. 1c). High-definition optical coherence tomography (HD-OCT; Skintell®; Agfa Gevaert, Antverpen, Belgium) showed a skin cavity (Fig. 2a). Reflectance confocal microscopy (RCM; Vivascope 3000®, Caliber, New York, USA, distributed in Europe by MAVIG GmbH, München, Germany) showed the roundish structure observed under dermoscopy much better (Fig. 2b) and identified an additional polycyclic intermediate reflecting symmetric structure (Fig. 2c).

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Presence of cutaneous human papillomavirus DNA in squamous cell carcinoma of the scalp: a case series

Abstract

Various hypotheses have been put forward on the pathogenesis of squamous cell carcinoma (SCC), including chronic sun damage and exposure to ultraviolet radiation (UVR), chemicals, smoking, immunosuppressive medications and human papillomavirus (HPV)1.

It is well known that alpha (mucosal) HPV types, in particular HPV-16 and HPV-18, are etiologically correlated to cervical cancer and other types of anogenital carcinoma and oropharyngeal papillomatosis1. On the contrary, the pathogenesis of squamous cell carcinoma (SCC) outside the mucosae remains obscure, and the link with HPVs has been demonstrated only in the epidermodysplasia verruciformis context2-3.

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Metastatic recurrence of 17-years relapse-free melanoma during anti-TNFa therapy

Abstract

Tumor-necrosis-factor-alpha (TNFa) is an innate cytokine involved in regulating inflammation, immunity, tumorigenesis, and apoptosis. TNFa inhibitors became a powerful tool in the treatment of auto-immune inflammatory diseases such as Crohn's disease, ulcerative colitis, rheumatoid arthritis and psoriasis. Known side effects of TNFa inhibitors range from infections to malignancies [1].

We report a male patient who was in 1998 at age 36 diagnosed with amelanotic melanoma of the leg, Breslow thickness 11.0 mm, no ulceration.

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Dermoscopy of uncommon variants of dermatofibrosarcoma protuberans

Abstract

Darier-Ferrand dermatofibrosarcoma protuberans (DFSP) is a locally aggressive fibrohistiocytic tumour with a low metastatic potential.1 Because of its rarity, slow progression and lack of early clinical clues, the diagnosis of DFSP is often delayed. Classical DFSP clinically appeared like an indurated, irregularly-shaped plaques exhibiting flesh to reddish-brown colour. Some lesions also showed thin teleangectasia on the surface (Fig. 1, a).

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Discovery of skin lymphocytes was a game changer in experimental dermatology

Abstract

A substantial part of ongoing research in experimental dermatology focuses on skin T cells – for that reason we find important to highlight the pioneering work of Jan D. Bos et al. from 1987 (The skin immune system (SIS): Distribution and immunophenotype of lymphocyte subpopulations in normal skin). This key article set the record straight, once and for all, about the presence of lymphocytes in healthy skin; characterized the immunophenotypes of subpopulations, quantified these cells, and studied their location. It was perhaps the critical discoveries made by Bos et al. that fueled the scientific community's interest in skin lymphocytes, contributing to a new generation of cutaneous immunology research. We briefly describe additional scientific breakthroughs made since 1987. Nonetheless, the study of cutaneous lymphocytes remains essential to understand the relationship of these cells to human diseases, and to develop therapies that can be leveraged to selectively mobilize, enhance or deplete these cells.

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