Bone regeneration enhancement by ultra-violet (UV) treatment for uHA/PLLA absorbable mesh

Publication date: Available online 12 February 2017
Source:Journal of Cranio-Maxillofacial Surgery
Author(s): Hiroumi Ikawa, Akinori Moroi, Kunio Yoshizawa, Yuriko Saida, Asami Hotta, Takamitsu Tsutsui, Kenichi Fukaya, Ryota Hiraide, Akihiro Takayama, Tatsuya Tsunoda, Yuki Saito, Koichiro Ueki
PurposeThe purpose of this study was to evaluate the effect of bone regeneration enhancement by ultra-violet (UV) treatment of an unsintered hydroxyapatite (u-HA)/poly-L-lactic acid (PLLA) mesh in rabbitnasal bone.Materials and methodsThirty adult male Japanese white rabbits (12-16 weeks, 2.5-3.0 kg) were used in this study. After incising along the nasal bone, 6 × 6 × depth 1 mm two bone defects were made on both sides. In the UV group (n = 30), the defects were covered with uHA/PLLA mesh, treated by UV (wavelength 172 nm, tube wall illumination 13 mW/cm2, period 8 min, intensity 6.26J/cm2) and screwed. In the control group (n = 30), untreated uHA/PLLA was applied in a similar manner. The rabbits were sacrificed at 1, 2, 3, 4 and 6 weeks postoperatively, and formalin-fixed specimens were frozen. The specimens were stained with hematoxylin and eosin. For immunohistochemical analysis, the specimens were treated with anti-alkaline phosphatase (ALP). Finally, bone ratio and ALP expression were evaluated microscopically.ResultsThe UV group had a significantly higher number of ALP stained cells than the control group after 1, 2, and 3 weeks (P < 0.05). The bone ratio was also significantly higher in the UV group than in the control group after 1, 2, 3, and 4 weeks (P < 0.05).ConclusionThis study suggests that bone regeneration can be enhanced by UV treatment using an uHA/PLLA mesh.



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