Αρχειοθήκη ιστολογίου

Αλέξανδρος Γ. Σφακιανάκης
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5
Άγιος Νικόλαος Κρήτη 72100
2841026182
6032607174

Δευτέρα 9 Μαΐου 2022

The Effect of Influenza Virus on The Metabolism of Peripheral Blood Mononuclear Cells with Metabolomics Approach

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Abstract

Respiratory viruses led to many deaths and hospitalization per year in the world. Influenza virus is one of the most important respiratory viruses. Recently, the metabolic studies in viral infections have been widely considered by scientists. Metabolomics states the metabolites present in a living organism under certain conditions. In this study, peripheral blood mononuclear cells were spinoculated using virus produced by the Madin-Darby Canine Kidney cell culture system and cells were harvested following spinoculation by influenza virus. Isolation of peripheral blood mononuclear cells was performed by Ficoll-Paque density gradient centrifugation. Metabolites were extracted using organic and Water approaches. Metabolic profiling was performed by a non-targeted using LC-MS/MS technique. Multivariate analysis methods used to determine the main variables. the metabolic pathways involved were determined using databases. Results of present study showed change in biosynthesis pathways such as lipids, polyamines, catecholamines and vitamins. Findings also showed that it is possible to explain the process of inflammation caused by the influenza virus by studying the metabolism of immune cells.

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Perceptions of NZ orthodontists and periodontists on the management of gingival recession in orthodontic patients

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ABSTRACT

Background

This study aimed to investigate the perceptions and opinions of orthodontists and periodontists on the management of gingival recession in orthodontic patients.

Methods

An online survey was sent to 29 periodontists and 80 orthodontists registered and currently practising in New Zealand. All participants answered questions about the timing and clinical indications of mucogingival surgeries in orthodontic patients diagnosed with mucogingival deformities.

Results

Most periodontists and orthodontists believed that gingival grafts should ideally be performed after orthodontic treatment. In clinical practice, 40% of periodontists indicated that they would receive referrals after completion of orthodontic treatment. However, 29.6% of orthodontists indicated that they would refer to a periodontist before orthodontic treatment in clinical practice. The most crucial factor that affected periodontists' decision making was 'evidence-based guidelines' (35.0%), followed by 'clinical experience' (30.0%) and 'patient concerns' (15.0%). All four factors of 'gingival phenotype', 'presence of gingival recession', 'amount of keratinised tissue' and 'planning specific tooth movements' were equally considered by orthodontists regarding their decision-making.

Conclusions

The majority of the surveyed New Zealand periodontists and orthodontists expressed a belief that the ideal timing for the management of gingival recessions would be after the completion of orthodontic treatment. © 2022 Australian Dental Association.

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Identification of non-synonymous variations in ROBO1 and GATA5 genes in a family with bicuspid aortic valve disease

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Journal of Human Genetics, Published online: 09 May 2022; doi:10.1038/s10038-022-01036-x

Identification of non-synonymous variations in ROBO1 and GATA5 genes in a family with bicuspid aortic valve disease
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Avoidance of cutaneous adverse drug reactions induced by antiepileptic drugs based on pharmacogenomics

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Journal of Human Genetics, Published online: 09 May 2022; doi:10.1038/s10038-022-01040-1

Avoidance of cutaneous adverse drug reactions induced by antiepileptic drugs based on pharmacogenomics
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Particulate matter (PM10) induces in vitro activation of human neutrophils, and lung histopathological alterations in a mouse model

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