The Hearing Journal

	​​​​​​​Get ready for Fall with The Hearing Journal's September issue! Online and Mobile Audiology Screenings Gain Market Recognition The popularity of online and mobile hearing tests has been gaining momentum, but will this increasingly sophisticated technology ever replace in-person audiometric testing? And what are its implications in the future of audiology?​ Hearing Loss Prevention Drug is Closer to Reality Have you heard of the potentially first-in-class drug ORC-13661? Dr. Edwin W. Rubel, founding Director of the Virginia Merrill Bloedel Hearing Research Center, explores hearing and balance disorders that may be addressed with a drug currently being developed with Oricula Therapeutics, LLC. Why Does Medicare Cover Cochlear Implants But Not Hearing Aids?​ The Hearing Journal's Editorial Board Chairperson, Dr. Fan-Gang Zeng, braves the issues surrounding the vast difference between Medicare coverage of CI and hearing aids. Advances in Optogenetic-based Auditory Implants Auditory implants are great options for those who do not benefit from cochlear implants, but recent developments in optogenetics show even greater potentials. The Hearing Aid 'Effect' as a Function of Race and Gender How prevalent is the "hearing aid effect"? This original study examines the current state of the dubbed "hearing aid effect," and how race and gender play in the equation. The Three A's of Hearing Health Care  In light of the groundbreaking NASEM report titled "Hearing Health Care for Adults," Dr. Barbara Weinstein suggests ways to integrate the NASEM recommendations in clinical practice.  ​ … And much more. ​  Want to read on the go? We have an app for that! bit.ly/AppHearingJ The Hearing Journal September 2016 - Volume 69 - Issue 9 pp: 26-48 View Contributor Index Cover Story Online and Mobile Audiology Screenings Gain Market Recognition Hocevar, Robin Editorial Hearing Loss Prevention Drug is Closer to Reality Rubel, Edwin W. Journal Club Advances in Optogenetic-based Auditory Implants Kozin, Elliott D.; Lee, Daniel J. Golden Rules The Three A's of Hearing Health Care Weinstein, Barbara E. Views and Vision Views and Vision Clinical Consultation Symptom: Ear Canal Mass and Pain Djalilian, Hamid R. Hearing Matters Confident, Concise Communication Kraus, Nina; White-Schwoch, Travis Original Research The Hearing Aid 'Effect' as a Function of Race and Gender McManus, Hilary; Salbod, Stephen; Berg, Abbey L. Tinnitus Research Tinnitus and Its Relation to Emotion, Exercise Husain, Fatima T. Viewpoint Why Does Medicare Cover Cochlear Implants But Not Hearing Aids? Zeng, Fan-Gang Hearing and Phonological Awareness Phonological Awareness in Children with Hearing Loss Rakhshanfadaee, Ali; Salehi, Masoud Manufacturers News Manufacturers News Disclaimer: This version of the table of contents may be incomplete. To view the official complete table of contents, please navigate your web browser to the journal's website at The Hearing Journal.

Alexandros Sfakianakis
Anapafseos 5 . Agios Nikolaos
Crete.Greece.72100
2841026182

6948891480

alsfakia@gmail.com

Current Opinion in Otolaryngology & Head and Neck Surgery

Published Ahead-of-Print The following 8 items were added to PAP on 8/31/2016. Review of adult laryngotracheal stenosis: pathogenesis, management, and outcomes. Rosow, David E.; Barbarite, Eric REVIEW: PDF Only Physical and virtual modelling of the head and neck for surgical simulation and training. Dirven, Steven; Allen, Jacqueline REVIEW: PDF Only Pediatric unilateral sensorineural hearing loss: implications and management. Dornhoffer, James R.; Dornhoffer, John L. REVIEW: PDF Only Editorial. Allen, Jacqui EDITORIAL: PDF Only Laryngeal framework surgery: current strategies. Hess, Markus M.; Fleischer, Susanne REVIEW: PDF Only Cricopharyngeal function or dysfunction: what's the deal?. Allen, Jacqui REVIEW: PDF Only Autoimmune swallowing disorders. Amos, Janine; Baron, Anthony; Rubin, Adam D. REVIEW: PDF Only Tissue engineering in the larynx and airway. Hertegård, Stellan REVIEW: PDF Only Current Opinion in Otolaryngology & Head and Neck Surgery

Alexandros Sfakianakis
Anapafseos 5 . Agios Nikolaos
Crete.Greece.72100
2841026182

6948891480

alsfakia@gmail.com

City, Culture and Society

City, Culture and Society  Volume 7, Issue 3 ,  Pages 123-196, September 2016  Editorial Board     Pages IFC    Original Research Articles Fragmentation and solidarity in the artistic milieu of contemporary Paris: A perspective from Emile Durkheim   Original Research Article  Pages 123-128  Christian Morgner  Rituals and the participation of urban form: Informal and formal image making processes   Original Research Article  Pages 129-138  Sukanya Krishnamurthy  Lost in protest, found in segregation: Divided cities in the light of the 2015 "Οχι" referendum in Greece   Original Research Article  Pages 139-148  Kostas Rontos, Efstathios Grigoriadis, Adele Sateriano, Maria Syrmali, Ioannis Vavouras, Luca Salvati  Spatialisation of selves: Religion and liveable spaces among Hindus and Muslims in the walled city of Ahmedabad, India   Original Research Article  Pages 149-154  Aparajita De  Commodification of art versus creativity: The Antagonist Art Movement in the expanding arts scene of New York City   Original Research Article  Pages 155-160  Ruth Trumble, Micheline van Riemsdijk  A typology of network city in a socio-economically disadvantaged region   Original Research Article  Pages 161-168  Yoon-Young Jeong, Tae-Heon Moon, Jung Hoon Han  An environmental and social approach in the modern architecture of Brazil: The work of Lina Bo Bardi   Original Research Article  Pages 169-185  Steffen Lehmann  Progressing urban development and life quality simultaneously   Original Research Article  Pages 186-193  Issa Ebrahimzadeh, Abdol Aziz Shahraki, Ali Akbar Shahnaz, Ayoub Manouchehri Myandoab    Urban Creativity Forum Urban regeneration & Valletta 2018     Pages 194-195  Karsten Xuereb

Alexandros Sfakianakis
Anapafseos 5 . Agios Nikolaos
Crete.Greece.72100
2841026182

6948891480

alsfakia@gmail.com

Effects of PTEN gene silencing on invasion and EMT in oral squamous carcinoma Tca8113 cells

Aim

To investigate the impact of silencing of the PTEN gene using siRNA on the invasion, proliferation, cell cycle, and epithelial-mesenchymal transition of the Tca8113 cell line.

Methods

The established Tca8113 cell model with siRNA interference to silence the PTEN gene was used. The transfection efficiency was examined by RT-qPCR and Western blot analysis. CCK-8 assay was utilized to analyze the proliferation of Tca8113 cells and cell invasion was evaluated using a transwell assay. The cell cycle distribution was analyzed by flow cytometry. The protein expression levels of the epithelial-mesenchymal transition (EMT) markers E-cadherin and Vimentin and the EMT-related proteins β-catenin and TGF-β1 were analyzed by Western blot.

Results

The expression level of PTEN was significantly reduced in the PTEN-siRNA group. The invasiveness and proliferation rate of Tca8113 cells in the PTEN-siRNA group were significantly greater than those of the control and negative control groups. The expression levels of E-cadherin and β-catenin were reduced, whereas the expression levels of vimentin and TGFβ-1 were elevated in the PTEN-siRNA group compared with those of control and negative groups. These results were significantly different.

Conclusion

The silencing of PTEN by siRNA increased the proliferation and promoted cell invasion of Tca8113 cells. PTEN gene silencing may accelerate the EMT in Tca8113 cells.

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