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- Implementable Deep Learning for Multi‐sequence Pro...
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Πέμπτη 15 Σεπτεμβρίου 2016
Three-dimensional Nasolabial Morphologic Alterations Following Le Fort I
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Proliferation, behavior, and differentiation of osteoblasts on surfaces of different microroughness
Publication date: Available online 13 September 2016
Source:Dental Materials
Author(s): Oleh Andrukhov, Rebecca Huber, Bin Shi, Simon Berner, Xiaohui Rausch-Fan, Andreas Moritz, Nicholas D. Spencer, Andreas Schedle
ObjectivesTitanium surface roughness is recognized as an important parameter influencing osseointegration. However, studies concerning the effect of well-defined surface topographies of titanium surfaces on osteoblasts have been limited in scope. In the present study we have investigated how Ti surfaces of different micrometer-scale roughness influence proliferation, migration, and differentiation of osteoblasts in-vitro.MethodsTitanium replicas with surface roughnesses (Ra) of approximately 0, 1, 2, and 4μm were produced and MG-63 osteoblasts were cultured on these surfaces for up to 5 days. The effect of surface micrometer-scale roughness on proliferation, migration in time-lapse microscopy experiments, as well as the expression of alkaline phosphatase, osteocalcin, vascular-endothelial growth factor (VEGF), osteoprotegerin (OPG), and receptor activator of nuclear factor kappa-B ligand (RANKL) were investigated.ResultsProliferation of MG-63 cells was found to decrease gradually with increasing surface roughness. However, the highest expression of alkaline phosphatase, osteocalcin and VEGF was observed on surfaces with Ra values of approximately 1 and 2μm. Further increase in surface roughness resulted in decreased expression of all investigated parameters. The cell migration speed measured in time-lapse microscopy experiments was significantly lower on surfaces with a Ra value of about 4μm, compared to those with lower roughness. No significant effect of surface roughness on the expression of OPG and RANKL was observed.SignificanceThus, surfaces with intermediate Ra roughness values of 1–2μm seem to be optimal for osteoblast differentiation. Neither proliferation nor differentiation of osteoblasts appears to be supported by surfaces with higher or lower Ra values.
Graphical abstract
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Wear, strength, modulus and hardness of CAD/CAM restorative materials
Source:Dental Materials
Author(s): Nathaniel C. Lawson, Ritika Bansal, John O. Burgess
ObjectiveTo measure the mechanical properties of several CAD/CAM materials, including lithium disilicate (e.max CAD), lithium silicate/zirconia (Celtra Duo), 3 resin composites (Cerasmart, Lava Ultimate, Paradigm MZ100), and a polymer infiltrated ceramic (Enamic).MethodsCAD/CAM blocks were sectioned into 2.5mm×2.5mm×16mm bars for flexural strength and elastic modulus testing and 4mm thick blocks for hardness and wear testing. E.max CAD and half the Celtra Duo specimens were treated in a furnace. Flexural strength specimens (n=10) were tested in a three-point bending fixture. Vickers microhardness (n=2, 5 readings per specimen) was measured with a 1kg load and 15s dwell time. The CAD/CAM materials as well as labial surfaces of human incisors were mounted in the UAB wear device. Cusps of human premolars were mounted as antagonists. Specimens were tested for 400,000 cycles at 20N force, 2mm sliding distance, 1Hz frequency, 24°C, and 33% glycerin lubrication. Volumetric wear and opposing enamel wear were measured with non-contact profilometry. Data were analyzed with 1-way ANOVA and Tukey post-hoc analysis (alpha=0.05). Specimens were observed with SEM.ResultsProperties were different for each material (p<0.01). E.max CAD and Celtra Duo were generally stronger, stiffer, and harder than the other materials. E.max CAD, Celtra Duo, Enamic, and enamel demonstrated signs of abrasive wear, whereas Cerasmart, Lava Ultimate, Paradigm MZ100 demonstrated signs of fatigue.SignificanceResin composite and resin infiltrated ceramic materials have demonstrated adequate wear resistance for load bearing restorations, however, they will require at least similar material thickness as lithium disilicate restorations due to their strength.
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The ABCs of Perineal Streptococcal Dermatitis: Case Series and Review of the Literature
Perineal streptococcal dermatitis (PSD) is largely known to be caused by group A β-hemolytic Streptococcus (GAS). We would like to bring cases of non-GAS PSD to the attention of dermatologists, as there are implications for workup and therapy. We report 3 pediatric cases of PSD: 1 caused by GAS, 1 caused by group B β-hemolytic Streptococcus (GBS), and 1 associated with group C β-hemolytic Streptococcus (GCS). GBS and GCS are very rarely reported in pediatric cases of PSD. The literature on non-GAS PSD is reviewed, which additionally revealed several instances of PSD caused by group G β-hemolytic Streptococcus (GGS) and Staphylococcus aureus. GBS, GCS, GGS, and S aureus are significant causes of PSD to consider, particularly among adult patients, based on our encountered cases and the literature. If using rapid antigen tests to expedite the diagnosis of GAS, we recommend supplementing with a lesional swab for bacterial culture and sensitivity as the rapid antigen test does not detect non-GAS organisms. Therapy should be tailored to the microbiologic cause.
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Anapafseos 5 . Agios Nikolaos
Crete.Greece.72100
2841026182
David Gergen Reunites NFL Purple People Eaters at the Upcoming LHF Screening at the University of Minnesota September 17, 2016 With ASBA Member Dr. Greg Ross as Treating Dentist
More than 20 retired players will visit the clinic Saturday for comprehensive screenings sponsored by National Football League Players Association.
Marking the first visits under a new care model developed by National Football League Players Association-sponsored Living Heart Foundation, more than 20 retired NFL players will visit University of Minnesota Health Signature Health and Wellness for comprehensive physicals, electrocardiograms, and lab tests this Saturday, Sept. 17.
The day of screenings is designed to help former players – like Purple People Eaters Gary Larsen and punter Greg Coleman – detect and treat the unintended health concerns that come with life after professional football.
"Wear and tear from high-caliber sports, be it football or otherwise, tend to appear as athletes age," said William Conroy, MD, medical director for Signature Health and Wellness. "Our program is a tailored, comprehensive care option to help busy people achieve and maintain good health, which is something the NFLPA wants for its members."
The Living Heart Foundation, under the auspices of the NFLPA, refers retired players to specialized care around the nation. It also develops education and wellness programming to raise awareness about conditions that often affect older athletes, such as obesity, hypertension, diabetes and heart disease. When left untreated, these conditions contribute to the shorter life expectancies of retired players.
Signature Health and Wellness, located in the University of Minnesota Health Clinics and Surgery Center, is the first designated brick-and-mortar provider to treat retired players referred by the NFLPA-funded Living Heart Foundation. The program will apply genomic testing to personalize treatment for patients, as well as partner with Pro Player Health Alliance to treat sleep apnea. David Gergen, President and CEO of Pro Player Health Alliance, selected Dr. Greg Ross as the dental sleep medicine expert to treat sleep apnea and related disorders with oral appliance therapy when medically indicated.
"More than two-thirds of adult Americans are obese or overweight, representing a burden of approximately $160 billion annually to the healthcare system," said Arthur J. Roberts, MD, retired cardiac surgeon, former NFL quarterback and Founder of the Living Heart Foundation. "Obesity and its underlying conditions play a significant role in the declining health of former NFL players, and we know University of Minnesota Health's academic clinicians have the expertise and skills to address these concerns."
Special guests at this symposium will be the four Minnesota Vikings Purple People Eaters (Carl Eller, Alan Page, Jim Marshall, Gary Larsen) and Maureen Bausch, CEO of the Minnesota Super Bowl Host Committee.
About University of Minnesota Health
University of Minnesota Health represents a collaboration between University of Minnesota Physicians and University of Minnesota Medical Center. Working together, we provide exceptional care in a wide range of specialties at our hospitals, clinics and in community‐based facilities throughout the region. Visit: http://www.mhealth.org
About the Living Heart Foundation
The Living Heart Foundation (LHF) is a nonprofit organization under IRS 501 (c) (3) code. The LHF was initially funded by a grant from the Edison Foundation. Subsequently, Funding sources and donations have been obtained from companies like Covidien, Pfizer, Meridian Health System, Siemens, Professional Athletes Foundation, and from individual sources. The LHF was established by Arthur J. Roberts, MD in April 2001 to combat sudden cardiac death and to provide cardiovascular risk stratification with early preventive intervention for cardiac, pulmonary, and metabolic conditions through on-site screening and integrated follow-up health programs. The LHF has published 10 peer review papers related to CV risk in college students & athletes, as well as former NFL athletes.
About Pro Player Health Alliance
Pro Player Health Alliance (PPHA) is an organization dedicated to helping former NFL players, through providing testing and treatment options for those who suffer from sleep apnea. Since launching in April 2012, over 400 players have been treated through PPHA's "Tackle Sleep Apnea" campaign. Also, the PPHA is dedicated to integrating education and raising sleep apnea awareness, in a fun and memorable way. In addition to the campaign, PPHA is dedicated to testing and getting people who suffer from sleep apnea treated.http://ift.tt/1Q9bGLg
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Τετάρτη 14 Σεπτεμβρίου 2016
Characteristics of dairy cows with a greater or lower risk of subacute ruminal acidosis: Volatile fatty acid absorption, rumen digestion, and expression of genes in rumen epithelial cells
Source:Journal of Dairy Science
Author(s): X. Gao, M. Oba
The objective of this study was to examine whether lactating dairy cows with a greater or lower risk of subacute ruminal acidosis (SARA) have differences in volatile fatty acid (VFA) absorption rate, expression of genes involved in VFA metabolism and intracellular pH regulation in rumen epithelial cells, and in situ carbohydrate digestibility in the rumen. We fed 14 ruminally cannulated mid-lactating dairy cows (119 ± 47.2 d in milk; body weight 640 ± 47.9 kg) a high-grain diet consisting of 30% forage ad libitum, with an 18-d diet adaptation and a 7-d sample and data collection period. Eight cows with the lowest acidosis index [area below pH 5.8 normalized for dry matter intake (DMI); 0.10 ± 0.16 pH × min/kg of DMI] and 5 with the highest acidosis index (3.72 ± 0.19 pH × min/kg of DMI) were classified as animals with lower risk (LS) and higher risk (HS) of SARA, respectively. Minimum (5.75 vs. 5.33) and mean rumen pH (6.33 vs. 5.98) were higher for LS than for HS cows. In addition, the duration and area of rumen pH below 5.8 was lower in LS cows (24.9 vs. 481 min/d; 2.94 vs. 102 pH × min/d). Although DMI, milk yield, and milk component yields did not differ, milk fat concentration tended to be higher for LS cows than for HS cows (3.36 vs. 2.93%). However, we observed no difference in VFA absorption rate between LS and HS cows. In situ starch and neutral detergent fiber digestibility were not different between LS and HS cows, but the relative mRNA abundance of lanosterol synthase (LSS) was higher for LS cows than for HS cows. In addition, the mRNA abundance of hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1) tended to be higher for LS cows than for HS cows. These results suggested that VFA absorption rate might not explain the difference in rumen pH between LS and HS cows in the current study, even though expression of some genes related to VFA metabolism in rumen epithelium may be associated with variation in the risk of SARA among lactating cows. This variation in the risk of SARA may not be attributed to differences in the capacity of rumen microbes to ferment carbohydrates, because in situ carbohydrate digestibility in the rumen was not different between cows with higher and lower risk of SARA.
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