Lidocaine represses the malignant behavior of lung carcinoma cells via the circ_PDZD8/miR-516b-5p/GOLT1A axis

xlomafota13 shared this article with you from Inoreader Lidocaine represses the malignant behavior of lung carcinoma cells via the circ_PDZD8/miR-516b-5p/GOLT1A axis Via Cellular and Molecular Biology Histol Histopathol. 2022 Jan 21:18423. doi: 10.14670/HH-18-423. Online ahead of print. ABSTRACT Lung carcinoma is the most prevalent malignancy in adults. Lidocaine (Lido) has been confirmed to exert an anti-tumor role in many human cancers. However, the role and underlying mechanism of Lido in lung carcinoma remain poorly understood. Cell proliferation ability, migration, invasion, and apoptosis were measured by Colony formation, 5-ethynyl-2'-deoxyuridine (EdU), Cell Counting Kit-8 (CCK-8), transwell, and flow cytometry assays. Circ_PDZD8, microRNA-516b-5p (miR-516b-5p), and Golgi transport 1A (GOLT1A) levels were detected by real-time quantitative polymerase chain reaction (RT-qPCR). Protein levels of proliferating cell nuclear antigen (PCNA) and GOLT1A were examined by western blot assay. The binding relationship between miR-516b-5p and circ_PDZD8 or GOLT1A was predicted by circular RNA Interactome or Starbase 3.0 and then verified by a dual-luciferase reporter assay. The biological roles of circ_PDZD8 and Lido on lung carcinoma cell growth were examined by the xenograft tumor model in vivo. Lido suppressed proliferation, migration, invasion, and induced apoptosis in lung carcinoma cells. Circ_PDZD8 and GOLT1A were increased, miR-516b-5p was decreased in lung carcinoma tissues and cell lines. Their expression presented the opposite trend in Lido-triggered lung carcinoma cells. Circ_PDZD8 might overturn the repression of Lido on cell growth ability and metastasis in this tumor. Mechanically, circ_PDZD8 might regulate GOLT1A expression by sponging miR-516b-5p. Circ_PDZD8 weakened the anti-lung carcinoma effect of Lido in vivo. Circ_PDZD8 might mitigate the inhibitory effect of Lido on tumor cell malignancy by modulating the miR-516b-5p/GOLT1A axis, providing a novel insight for lung carcinoma treatment. PMID:35060113 | DOI:10.14670/HH-18-423 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Radioiodination and Purification of [131I]β-CIT and [131I]FP-CIT with an Automated Radiosynthesizer

xlomafota13 shared this article with you from Inoreader Radioiodination and Purification of [<sup>131</sup>I]β-CIT and [<sup>131</sup>I]FP-CIT with an Automated Radiosynthesizer Via radioactive iodine Pharmaceuticals (Basel). 2022 Jan 14;15(1):96. doi: 10.3390/ph15010096. ABSTRACT Dopaminergic transporter (DAT) imaging with single photon emission computed tomography (SPECT) is used to diagnose Parkinson's disease and to differentiate it from other neurodegenerative disorders without presynaptic dopaminergic dysfunction. The radioiodinated tropane alkaloids [123I]FP-CIT and [123I]β-CIT enable the evaluation of the integrity of DATs. Commonly, the labeling o f these compounds is performed by electrophilic substitution of the alkylstannylated precursors with radioactive iodine and following purification by HPLC or solid phase extraction (SPE). This work presents the first radioiodination of β-CIT and FP-CIT with no carrier added [131I]NaI on a Scintomics GRP synthesis module. Free iodine-131 and impurities were removed by SPE over a C-18 Sep-Pak cartridge. We achieved a radiochemical yield of >75% and a radiochemical purity of >98% with both compounds. Our development of an automated synthesis on a commercially available synthesizer ensures robust and efficient labeling of [131I]FP-CIT and [131I]β-CIT starting with low concentrated radioiodine. PMID:35056152 | DOI:10.3390/ph15010096 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Imaging characteristics of hypertrophic pachymeningitis due to ANCA-associated vasculitis

xlomafota13 shared this article with you from Inoreader Imaging characteristics of hypertrophic pachymeningitis due to ANCA-associated vasculitis Via Auris Nasus Larynx Publication date: Available online 21 January 2022 Source: Auris Nasus Larynx Author(s): Taro Takagi, Masahiro Okada, Masashi Nakamura, Takahiro Hanari, Takahiro Nakata, Masato Teraoka, Naohito Hato View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

New European approval: Idecabtagene vicleucel in relapsed and/or refractory myeloma after at least 3 lines of treatment

xlomafota13 shared this article with you from Inoreader New European approval: Idecabtagene vicleucel in relapsed and/or refractory myeloma after at least 3 lines of treatment Via Association française pour l'étude du cancer Bull Cancer. 2022 Jan 18:S0007-4551(21)00686-X. doi: 10.1016/j.bulcan.2021.10.007. Online ahead of print. NO ABSTRACT PMID:35063182 | DOI:10.1016/j.bulcan.2021.10.007 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

The management and outcome of large volume liposarcomas encasing the sciatic nerve

xlomafota13 shared this article with you from Inoreader The management and outcome of large volume liposarcomas encasing the sciatic nerve Via Journal of plastic, reconstructive & aesthetic surgery : JPRAS J Plast Reconstr Aesthet Surg. 2021 Oct 22:S1748-6815(21)00505-2. doi: 10.1016/j.bjps.2021.09.070. Online ahead of print. ABSTRACT This paper outlines the oncological outcomes of patients with large volume liposarcomas of the posterior thigh who underwent nerve-preserving surgery utilising epineural dissection. Thirty-seven consecutive patients (group I) with a mean age of 66.2 (31-96) were surgically treated with a planned marginal resection and epineurectomy for liposarcoma with known sciatic nerve involvement between March 1997 and January 2015. The mean follow-up was 79 months (15-192). All patients underwent multidisciplinary team (MDT) pre-operative assessment and staging, with follow-up in Sarcoma Clinic. Pre-operative function was assessed by applying the Toronto extremity salvage score (TESS). Oncological and functional outcomes were recorded. In grades 1, 2, and 3, 24, 6, and 7 liposarcomas, respectively, were included with m ean volume 1859 cm3. Sciatic nerve involvement extended for 13-30 cm; in one case, the nerve was abutting the tumour throughout its length. Soft tissue reconstructive surgery was required in three cases. The remainder underwent direct primary closure. Seventeen patients underwent post-operative adjuvant radiotherapy 46-60 Gy and three received chemotherapy. There was local recurrence of disease in three patients. One patient had post-radiation wound breakdown treated non-operatively. Three patients died of an unrelated cause. When compared to a cohort of 37 patients without sciatic nerve involvement (group II), there were no significant differences in local and systemic recurrence rate or post-operative survival. In conclusion, sciatic nerve-preserving surgery is both possible and safe when using a planned epineural dissection in large volume tumours encasing the sciatic nerve. PMID:35063385 | DOI:10.1016/j.bjps.2021.09.070 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Exploration of chest wall perforator vascular anatomy on standard breast MRI: A potential aid to chest wall perforator flap planning

xlomafota13 shared this article with you from Inoreader Exploration of chest wall perforator vascular anatomy on standard breast MRI: A potential aid to chest wall perforator flap planning Via Journal of plastic, reconstructive & aesthetic surgery : JPRAS J Plast Reconstr Aesthet Surg. 2021 Dec 1:S1748-6815(21)00639-2. doi: 10.1016/j.bjps.2021.11.092. Online ahead of print. ABSTRACT Local chest wall perforator flaps (CWPFs) are a volume replacement technique permitting breast-conserving surgery in patients who otherwise may require a mastectomy. These flaps are based on one or more perforating arteries arising from the lateral chest wall that travel through the soft tissue and into the sub-dermal plexus to perfuse the flap. Examples include the lateral intercostal and lateral thoracic artery perforators (LICAP and LTAP, respectively). Cross-sectional imaging of perforating vessels is not routinely performed, and vessels are mapped pre- and peri-operatively using a hand-held acoustic doppler device. As many breast cancer patients undergo pre-operative MRI scanning for oncological purposes, we investigated the role of MRI in mapping the vascular anatomy to aid with the surgical planning o f CWPFs. We collated data retrospectively on a cohort of breast cancer patients who underwent breast MRI as part of routine pre-operative imaging. Axial 3D high-resolution dynamic contrast-enhanced MRI sequences with multiplanar reconstructions were analysed by a consultant radiologist. The presence and calibre of lateral chest wall perforator vessels were assessed. Fifty patients were suitable for inclusion. A consistent pattern of lateral chest wall vasculature was observed. Forty-eight patients (96%) demonstrated a bilateral lateral thoracic artery (LTA) descending inferiorly along the chest wall with two-thirds of these communicating with perforating intercostal vessels. True independent LICAP vessels were identified in six patients. From our observations, lateral CWPFs are dependent on an intricate intercommunication between intercostal vessels and the LTA which in turn supply perforators to the lateral chest wall donor site. PMID:35063384 | DOI:10.1016/j.bjps.2021.11.092 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Intractable Bleeding Following an Awake Bedside Injection Laryngoplasty in a Patient on KVAD ECMO

xlomafota13 shared this article with you from Inoreader Intractable Bleeding Following an Awake Bedside Injection Laryngoplasty in a Patient on KVAD ECMO Via Journal of Voice Awake injection laryngoplasty (IL) is becoming increasingly utilized in the inpatient setting, especially as a therapeutic option for patients with vocal fold immobility immediately following cardiothoracic surgery. While prior studies consistently demonstrate complication rates below 3%, significant bleeding has not been reported as a major complication in any awake IL case series. The objective of this report is to highlight a case of intractable bleeding following awake inpatient bedside IL in a patient on KVAD (Koji Takeda Ventricular Assist Device) extracorporeal membrane oxygenation (ECMO). View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.