The enteric nervous system and the musculature of the colon are altered in patients with spina bifida and spinal cord injury

Abstract

Neurogenic bowel dysfunction occurs in a large percentage of adult patients with spina bifida (SB) and spinal cord injury (SCI), significantly affecting their quality of life. Although bowel motility is autonomously regulated by the enteric nervous system (ENS), disruption of the modulation of the ENS by extrinsic innervation as present in many patients with SB and SCI might lead to motility disorders. In order to gain insight in the pathophysiology, we studied histological changes of the neuromuscular structures in the colon of SB and SCI patients. Archival colon tissue blocks from SB (n = 13) and SCI (n = 34) patients were collected nationwide in The Netherlands and compared with control samples (n = 16). Histological (semiquantitative) evaluation of the ENS, the network of interstitial cells of Cajal (ICC), and the muscularis propria was performed using hematoxylin and eosin, periodic acid Schiff, and elastic von Gieson staining, and immunohistochemistry with antibodies against HuC/D, calretinin, S100, CD117, α-smooth muscle actin, and desmin. Compared to controls, SB and SCI patients showed neuronal loss and decreased nerve fiber density in the myenteric plexus. Lower nerve fiber density was significantly more often found in patients with severe bowel dysfunction. Other major findings were loss of ICCs around the myenteric plexus and fibrosis in the longitudinal muscle layer. Altered histology of the ENS may explain abnormal intestinal motility in SB and SCI patients. Furthermore, loss of myenteric nerve fibers (including enteric glial cells) may play a major role in the development of severe motility complaints.

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Analysis of the variation in low-level laser energy density on the crushed sciatic nerves of rats: a morphological, quantitative, and morphometric study

Abstract

The objective of this study was to evaluate three energy densities of low-level laser therapy (LLLT, GaAlAs, 780 nm, 40 mW, 0.04 cm²) for the treatment of lesions to peripheral nerves using the sciatic nerve of rats injured via crushing model (15 kgf, 5.2 MPa). Thirty Wistar rats (♂, 200–250 g) were divided into five groups (n = 6): C—control, not injured, and irradiated; L0—injured nerve without irradiation; L4—injured nerve irradiated with LLLT 4 J/cm² (0.16 J); L10—injured nerve irradiated with LLLT 10 J/cm² (0.4 J); and L50—injured nerve irradiated with LLLT 50 J/cm² (2 J). The animals were sacrificed 2 weeks after the injury via perfusion with glutaraldehyde (2.5%, 0.1 M sodium cacodylate buffer). The nerve tissue was embedded in historesin, cut (3 μm), mounted on slides, and stained (Sudan black and neutral red). The morphological and quantitative analysis (myelin and blood capillary densities) and morphometric parameters (maximum and minimum diameters of nerve fibers, axon diameter, G-ratio, myelin sheath thickness) were assessed using the ImageJ software. ANOVA (parametric) or Kruskal-Wallis (nonparametric) tests were used for the statistical analysis. Groups L0, L4, L10, and L50 exhibited diminished values of all the quantitative and morphometric parameters in comparison to the control group. The morphological, quantitative, and morphometric data revealed improvement after injury in groups L4, L10, and L50 (irradiated groups) compared to the injured-only group (L0); the best results, in general, were observed for the L10 group after 15 days of nerve injury.

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The photobiomodulation effect of higher-fluence 808-nm laser therapy with a flat-top handpiece on the wound healing of the earthworm Dendrobaena veneta : a brief report

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Volumetric assessment of alveolar clefts: a literature review

Abstract

Objective

To conduct a review of the literature on methods for volumetric assessment of alveolar clefts and ascertain which methods are most precise to guide future research.

Methods

The PubMed and MEDLINE databases were searched for English-language reports of clinical and in vitro studies with detailed descriptions of imaging modality, sample characteristics, and method for measurement of alveolar cleft volume.

Results

The search strategy yielded 34 articles, of which 14 were selected for in-depth analysis. According to the findings of computed tomography (CT)-based studies, the CT modalities and software employed for assessment were deemed reliable and satisfactory.

Conclusions

Measurement of alveolar cleft volume by the proposed methods and using the equipment and software employed in the analyzed studies has high efficacy and efficiency, and can be safely used for cleft lip and palate treatment planning.

This article is protected by copyright. All rights reserved.

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Maxillary Expansion and Mandibular Setback Surgery With and Without Mandibular Anterior Segment Osteotomy to Correct Mandibular Prognathism With Obstructive Sleep Apnea.

Mandibular prognathism is usually treated with mandibular setback surgery. However, this approach reduces the pharyngeal airway space, and can aggravate obstructive phenomena in patients with obstructive sleep apnea (OSA). While maxillary expansion is known to lead to an increase in the pharyngeal airway volume (PAS), its effect on the PAS in mandibular setback surgery has not yet been reported. The authors report a surgical approach with maxillary expansion in 2 patients with mandibular prognathism that was accompanied by OSA: maxillary midsagittal expansion with minimum maxillary advancement and minor mandibular setback without mandibular anterior segmental osteotomy (ASO) or major mandibular setback with mandibular ASO. Preoperative and postoperative computed tomography and polysomnography indicated that OSA was improved and pharyngeal airway space was increased or sustained, and the prognathic profile could be corrected to an acceptable facial esthetic profile. In summary, maxillary transversal expansion and mandibular setback with or without mandibular ASO can be successfully applied to treat mandibular prognathism with OSA. (C) 2017 by Mutaz B. Habal, MD.

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Prevalence and Associated Factors of Mandibular Asymmetry in an Adult Population.

Background: This study aimed to estimate the prevalence of mandibular asymmetry and investigate some associated factors. Methods: Tomographic images of 952 individuals aged from 18 to 75 years old were analyzed. The investigated outcome was mandibular asymmetry. The explanatory variables included gender, age, absence of posterior teeth, and sagittal jaw relationship of the individuals. Statistical analyses included the chi-squared and the Poisson regression with robust variance. Results: Mandibular asymmetry was present in 17.4% of the sample (95% confidence interval 15.2-20.0). In the bivariate analysis, there was an association between the variables age and sagittal jaw relationship with mandibular asymmetry (P = 0.026 and P = 0.018, respectively). However, in the adjusted regression model, the association with age was not maintained, occurring only an association between sagittal jaw relationship and mandibular asymmetry (P = 0.045), with significant difference between individuals with skeletal Class II and skeletal Class III (P = 0.013). Conclusion: Mandibular asymmetry was not independently associated with sex, age, or absence of posterior teeth. The only verified independent association was between mandibular asymmetry and sagittal jaw relationship. (C) 2017 by Mutaz B. Habal, MD.

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Morphology of the Levator Veli Palatini Muscle in Adults With Repaired Cleft Palate.

The purpose of this study was to examine differences in levator veli palatini (levator) morphology between adults with repaired cleft palate and adults with noncleft anatomy. Fifteen adult participants (10 with noncleft anatomy, 5 with repaired cleft palate) underwent 3-dimensional (3D) static magnetic resonance imaging (MRI). Image analyses included measures of total muscle volume and the circumference and diameter at 6 points along the length of the muscle. Differences between groups were analyzed using independent sample Mann-Whitney U tests ([alpha]

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