Low-level laser irradiation modifies the effect of hyperglycemia on adhesion molecule levels

Abstract

Endothelium plays a key role in maintaining vascular homeostasis by secreting active factors involved in many biological processes such as hemostasis, angiogenesis, and inflammation. Hyperglycemia in diabetic patients causes dysfunction of endothelial cells. Soluble fractions of adhesion molecules like sE-selectin and vascular cell adhesion molecule (sVCAM) are considered as markers of endothelial damage. The low-level laser therapy (LLLT) effectively supports the conventional treatment of vascular complications in diabetes, for example hard-to-heal wounds in patients with diabetic foot syndrome. The aim of our study was to evaluate the effect of low-energy laser at the wavelength of 635 nm (visible light) and 830 nm (infrared) on the concentration of adhesion molecules: sE-selectin and sVCAM in the supernatant of endothelial cell culture of HUVEC line. Cells were cultured under high-glucose conditions of 30 mM/L. We have found an increase in sE-selectin and sVCAM levels in the supernatant of cells cultured under hyperglycemic conditions. This fact confirms detrimental influence of hyperglycemia on vascular endothelial cell cultures. LLLT can modulate the inflammation process. It leads to a decrease in sE-selectin and sVCAM concentration in the supernatant and an increase in the number of endothelial cells cultured under hyperglycemic conditions. The influence of LLLT is greater at the wavelength of 830 nm.

https://ift.tt/2HPg3PO

Classification proposal for fractures of the processus condylaris mandibulae

Abstract

Objective

The current classification of mandibular condyle fractures as basal, low neck, and high neck as reported by Loukota et al. (Br J Oral Maxillofac Surg 43:72–73, 2005) and Neff et al. (Craniomaxillofac Trauma Reconstr 7:S44–S58, 2014) has a weakness. Nearly no high-neck fractures are reported (they are typically classified as type C head fractures) contrary to basal condylar fractures, which are overestimated (nearly all low-neck fractures are classified as basal). The aim of this study is to present a modified AO/SORG classification of mandibular condyle fractures.

Material and methods

A new arrangement of the reference lines is proposed because the fracture lines are mainly oblique in this region. The proposed classification was validated using a series of 84 cases that were treated surgically.

Results

The diagnoses using the proposed new classification system significantly differed from those based on the old system (p < 0.005). All basal fractures in the new classification system were also classified as basal in the old system. The same was true for type C head fractures. The differences were found for low-neck fractures (4 of 84 diagnoses differed between the old and new classifications, i.e., they were previously classified as basal fractures) and high-neck fractures (3 of 84 fractures were diagnosed as low-neck fractures or type C head fractures using the old classification).

Conclusion

The epidemiology of the condyle injury should be based on a classification, which reveals types of fractures which are represented by factually and frequently observed cases. That is why a relatively common AO/SORG classification can be modified for the benefit of assessing incidences of high-neck and low-neck fractures.

Clinical relevance

Considering that the treatment of the high-neck fractures is much technically complicated than the low-neck ones, this will have an influence on the management of trauma to the area.

https://ift.tt/2HQ4jg3

Involvement of acute neuroinflammation in postoperative delirium-like cognitive deficits in rats

Abstract

Purpose

The purpose of this study was to investigate the age-, time-, and brain region-dependent postoperative neuroinflammatory trajectory, and its association with neurocognitive outcomes in rats.

Methods

Adult and aged rats were randomly assigned to one of three groups: control, isoflurane anesthesia alone, and isoflurane anesthesia with abdominal surgery. On either postoperative day 2 (early phase) or 7 (late phase), all rats were tested for trace and context fear memory retention after acquisition of trace fear conditioning. Freezing behavior was used as an index of fear memory. Following the cognitive testing, the levels of pro-inflammatory cytokines in several brain regions were measured using enzyme-linked immunosorbent assay (n = 8 in each group).

Results

In the early postoperative period, surgery under isoflurane anesthesia induced acute neuroinflammation along with related trace and context memory dysfunction. Such acute neuroinflammatory responses were comparably observed in both adult and aged animals, whereas the aged rats were more likely to exhibit behavioral changes. On the other hand, in the late postoperative period, neither neuroinflammation in all tested brain regions nor concomitant memory decline were found in adult animals. Significant neuroinflammation was detected only in the hippocampus of aged rats, which was associated with context, but not trace memory dysfunction.

Conclusion

Our findings indicate that surgery-induced acute, transient, brain-wide neuroinflammation may be involved in the pathogenesis of the postoperative delirium-like cognitive deficits in rats. Furthermore, neuroinflammation may convert from acute to chronic in an age- and hippocampal-specific manner, likely resulting in the development of sustained cognitive dysfunction.

https://ift.tt/2HSYoCP

Optical coherence tomography to evaluate variance in the extent of carious lesions in depth

Abstract

Evaluation of variance in the extent of carious lesions in depth at smooth surfaces within the same ICDAS code group using optical coherence tomography (OCT) in vitro and in vivo. (1) Verification/validation of OCT to assess non-cavitated caries: 13 human molars with ICDAS code 2 at smooth surfaces were imaged using OCT and light microscopy. Regions of interest (ROI) were categorized according to the depth of carious lesions. Agreement between histology and OCT was determined by unweighted Cohen's Kappa and Wilcoxon test. (2) Assessment of 133 smooth surfaces using ICDAS and OCT in vitro, 49 surfaces in vivo. ROI were categorized according to the caries extent (ICDAS: codes 0–4, OCT: scoring based on lesion depth). A frequency distribution of the OCT scores for each ICDAS code was determined. (1) Histology and OCT agreed moderately (κ = 0.54, p ≤ 0.001) with no significant difference between both methods (p = 0.25). The lesions (76.9% (10 of 13)) _were equally scored. (2) In vitro, OCT revealed caries in 42% of ROI clinically assessed as sound. OCT detected dentin-caries in 40% of ROIs visually assessed as enamel-caries. In vivo, large differences between ICDAS and OCT were observed. Carious lesions of ICDAS codes 1 and 2 vary largely in their extent in depth.

https://ift.tt/2jruviG

“Non-invasive Follicular Thyroid Neoplasm with Papillary like Nuclear Features”: An Institutional Retrospective Study with Re-nomenclature and Re-classification

Abstract

The cumulative efforts of pioneers in field of Thyroid pathology have resulted in proposed reclassification of Encapsulated Follicular Variant of Papillary Thyroid Carcinoma (EFVPTC) to "Non-invasive follicular thyroid neoplasm with papillary-like nuclear features" (NIFTWP) due to indolent clinical behaviour. To study the spectrum of EFVPTCs in our institute and to reclassify them as NIFTWP using stringent morphological criteria. This is a retrospective study conducted from Jan 2014 to July 2017. Institutional records were accessed and out of 485 thyroidectomy cases, 35 cases of EFVPTC were taken into study and field's kappa was used as a means of statistical analysis. The study conducted on 35 cases of EFVPTCs using strict morphological criteria lead to re-nomenclature and re-classification of 30 out of these 35 cases, with a field's kappa value of 4.3. The present study, lead to re-nomenclature and re-classification of 30 cases of EFVPTC to NIFTWP and proved statistically that there is moderate level of Inter-observer agreement regarding the criteria used for this re-nomenclature and re-classification.

https://ift.tt/2HQSkem

The association with xerostomia from sialadenitis and the jaw osteonecrosis in head and neck cancer population: a nationwide cohort study

Abstract

Objectives

Determine the association between sialadenitis and osteonecrosis of the jaw (ONJ) in head and neck cancer (HNC) patients with varying severity of sialadenitis, treatment modalities, and cancer locations.

Materials and methods

A total of 40,168 HNC patients, including 1907 ONJ subjects and 7559 matched comparisons, were enrolled from a Longitudinal Health Insurance Database for Catastrophic Illness Patients of Taiwan between 2000 and 2006. The association with sialadenitis and ONJ was estimated by logic regression and presented as the odds ratio (OR) and 95% confidence intervals (CIs).

Results

The occurrence of sialadenitis increased the risk of ONJ by 2.55-fold in HNC patients (95% CI = 2.20–2.95). The ONJ incidence was proportion to sialadenitis severity (OR = 2.53 to 4.43). Irradiated HNC patients had a higher tendency to develop jaw necrosis (osteoradionecrosis, ORN) (OR = 5.05, 95% CI = 4.39–5.80). When combined with irradiation exposure, sialadenitis significantly induced the occurrence of ORN (OR = 8.94, 95% CI = 7.40–10.8), especially in oral cancer patients (OR = 15.9 95% CI = 12.5–20.3). The risk of ONJ increased with radiotherapy dosage and duration, except for nasopharyngeal cancer (NPC) patients.

Conclusions

There was a close association between sialadenitis and ONJ in the HNC population. The severity of sialadenitis was positive correlated to ONJ risk. Radiotherapy combined with sialadenitis significantly raised ORN incidence in HNC patients except for NPC patients.

Clinical relevance

HNC patients complained that xerostomia from sialadenitis might increase the risk to develop ONJ, especially among those who received radiotherapy.

https://ift.tt/2HOW8Aw

The impact of using a pneumatic contra-angle device on the lifespan of M-Wire- and Blue-treated instruments

Abstract

Objectives

To evaluate the cyclic fatigue resistance of Reciproc M-Wire R25 (VDW, Munich, Germany) and Reciproc R25 Blue instruments (VDW) driven by Direct® (VDW) contra-angle connected to an ordinary an air-driven motor or an electric motor and compare the results with those obtained by the Reciproc M-Wire R25 or Reciproc Blue R25 instruments driven by an electric torque-controlled motor using "RECIPROC ALL" preset program.

Materials and methods

Thirty Reciproc M-Wire R25 (25/0.08v) and 30 Reciproc Blue R25 (25/0.08v) instruments were used. Cyclic fatigue resistance was tested measuring the time to fracture and the number of cycles to fracture in an artificial stainless-steel canal with a 60° angle and a 5-mm radius of curvature. The Reciproc M-Wire and Reciproc Blue instruments were activated with a 6:1 reduction handpiece powered by a torque-controlled motor using "RECIPROC ALL" preset program, with Reciproc Direct® contra-angle powered by an ordinary air-driven motor or with Direct® contra-angle powered by an electric motor (n = 10). The fracture surface of all fragments was examined with a scanning electron microscope. The results were statistically analyzed using Student's t test and one-way ANOVA at a significance level of P < 0.05.

Results

Cyclic fatigue life and number of cycles to fracture were significantly higher for Reciproc Blue instruments than for Reciproc M-Wire instruments regardless of the activation mode (P < 0.05). Instruments driven by Direct® contra-angle powered by an electric or by an ordinary air-driven motor revealed significantly longer cyclic fatigue life and number of cycles to fracture than instruments driven by an electric torque-controlled motor using "RECIPROC ALL" preset program (P < 0.05).

Conclusions

Reciproc Blue instruments showed improved performance regarding fatigue resistance when compared to Reciproc M-Wire instruments. Instruments driven by Reciproc Direct® contra-angle showed higher cyclic fatigue life and number of cycles to fracture than instruments driven by an electric torque-controlled motor.

Clinical relevance

Recently, Reciproc Direct®, the world's first contra-angle handpiece with integrated reciprocating motion, has been launched in endodontic market. The present study showed improved cyclic fatigue life of endodontic instruments when activated by Reciproc Direct®.

https://ift.tt/2wfTrCG