Effects of Gynostemma pentaphyllum on spinal cord motor neurons and microglial cells in vitro

xlomafota13 shared this article with you from Inoreader Effects of Gynostemma pentaphyllum on spinal cord motor neurons and microglial cells in vitro Via histochem Acta Histochem. 2021 Aug 20;123(6):151759. doi: 10.1016/j.acthis.2021.151759. Online ahead of print. ABSTRACT The regenerative capability of spinal cord neurons is limited to impossible. Thus, experimental approaches supporting reconstruction/regeneration are in process. This study focused on the evaluation of the protective potency of an extract from Gynostemma pentaphyllum (GP), a plant used in traditional medicine with anti-oxidative and neuroprotective activities, in vit ro on organotypic spinal cord cultures, the motor-neuron-like NSC-34 cell line and the microglial cell line BV-2. Organotypic cultures were mechanically stressed by the slicing procedure and the effect of GP on motor neuron survival and neurite sprouting was tested by immunohistochemistry. NSC-34 cells were neuronal differentiated by using special medium. Afterwards, cell survival (propidium iodide/fluorescein diacetate labeling), proliferation (BrdU-incorporation), and neurite sprouting were evaluated. BV-2 cells were stimulated with LPS/interferon γ and subjected to migration assay and nanoparticle uptake. Cell survival, proliferation and the expression pattern of different microglial activation markers (cFOS, iNOS) as well as transcription factors (PPARγ, YB1) were analyzed. In organotypic cultures, high-dose GP supported survival of motor neurons and especially of the neuronal fiber network. Despite reduced neurodegeneration, however, there was a GP-mediated activation of astr o- and microglia. In NSC-34 cells, high-dosed GP had degenerative and anti-proliferative effects, but only in normal medium. Moreover, GP supported the neuro-differentiation ability. In BV-2 cells, high-dosed GP was toxic. In lower dosages, GP affected cell survival and proliferation when combined with LPS/interferon γ. Nanoparticle uptake, migration ability, and the transcription factor PPARγ, however, GP affected directly. The data suggest positive effects of GP on injured spinal motor neurons. Moreover, GP activated microglial cells. The dual role of microglia (protective/detrimental) in neurodegenerative processes required further experiments to enhance the knowledge about GP effects. Therefore, a possible clinical use of GP in spinal cord injuries is still a long way off. PMID:34425524 | DOI:10.1016/j.acthis.2021.151759 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Reliability and Correlations Between Overall Severity, Roughness and Breathiness in the Perception of Dysphonic Voices: Investigating Cognitive Aspects

xlomafota13 shared this article with you from Inoreader Reliability and Correlations Between Overall Severity, Roughness and Breathiness in the Perception of Dysphonic Voices: Investigating Cognitive Aspects Via Journal of Voice this study concerns the subjective perception of the quality of the voice, more particularly in the case of dysphonia. Our general objective is to study the perceptual mechanisms, which constitute Hirano's GRBAS multidimensional perceptual rating scale. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

92. Jahresversammlung der DGHNO-KHT 2021 erstmals im Online-Format – eine kritische Bewertung auf Basis der Teilnehmerevaluation

xlomafota13 shared this article with you from Inoreader 92. Jahresversammlung der DGHNO-KHT 2021 erstmals im Online-Format – eine kritische Bewertung auf Basis der Teilnehmerevaluation Via Laryngo-Rhino-Otologie Laryngorhinootologie DOI: 10.1055/a-1579-8096 Die 92. Jahresversammlung der DGHNO-KHC wurde im Mai 2021 erstmals aufgrund der Corona-bedingten neuen Rahmenbedingungen komplett in einem Online-Format durchgeführt. Den Teilnehmern aus den Reihen der Mitglieder, Gäste und Industriepartner wurde im Anschluss ein Evaluationsfragebogen der zuständigen Landesärztekammer Nordrhein zugesandt, der inhaltlich um Fragen zu dem neuen virtuellen Format ergänzt wurde. Die Publikation fasst die Auswertung der 187 ärztlichen Rückläufer (10 % der Gesamtteilnehmerzahl) und der 25 Industrieaussteller (60 % der Industrieaussteller) zusammen und stellt ein erstes Fazit auf. In der groben Betrachtung wurde der Kongress von den ärzt lichen und wissenschaftlichen Teilnehmern trotz komplett fehlender sozialer und sehr eingeschränkter fachlicher Interaktion überwiegend positiv bewertet. Auf Seiten der Industrie bot sich ein gegenteiliges Bild. Auf die Frage an die ärztlich/wissenschaftlichen Teilnehmenden nach dem zukünftigen Format der nächsten HNO-Jahreskongresse sprachen sich 16 % für einen reinen Online-Kongress, 67 % für einen Präsenzkongress mit Online-Elementen und lediglich 17 % für einen reinen Präsenzkongress aus. Auf die Frage an die Industrie, welche Art der Ausstellung in Zukunft bevorzugt würde, sprachen sich 68 % für die reine Präsenzausstellung im Rahmen eines Präsenzkongresses aus. 32 % konnten sich für Präsenz mit Online-Elementen erwärmen. Eine zukünftig komplette Online-Industrieausstellung wurde mit 0 % Zustimmung abgewählt. [...] Georg Thieme Verlag KG Rüdigerstraße 14, 70469 Stuttgart, Germany Article in Thieme eJournals: Table of contents  |  Abstract  |  Full text View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Abdomen and thorax clinical anatomy: from viscera morphology and vascularization to epidural and caudal anesthetic techniques

xlomafota13 shared this article with you from Inoreader Abdomen and thorax clinical anatomy: from viscera morphology and vascularization to epidural and caudal anesthetic techniques Via radiol anat Surg Radiol Anat. 2021 Aug 24. doi: 10.1007/s00276-021-02823-5. Online ahead of print. NO ABSTRACT PMID:34427734 | DOI:10.1007/s00276-021-02823-5 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Identification of Cellular Voids in the Human Otic Capsule

xlomafota13 shared this article with you from Inoreader Identification of Cellular Voids in the Human Otic Capsule Via Journal of the Association for Research in Otolaryngology Abstract The otic capsule consists of dense highly mineralized compact bone. Inner ear osteoprotegerin (OPG) effectively inhibits perilabyrinthine remodeling and otic capsular bone turnover is very low compared to other bone. Consequently, degenerative changes like dead osteocytes and microcracks accumulate around the inner ear. Osteocytes are connected via canaliculi and need a certain connectivity to sustain life. Consequently, stochastic osteocyte apoptosis may disrupt the osteocytic network in unsustainable patterns leading to widespread cell death. When studying bulk-stained undecalcified human temporal bone, large clusters of dead osteocytes have been observed. Such "cellular voids" may disrupt the perilabyrinthine OPG mediated remodeling inhibition possibly leading to local remodeling. In the common ear disease otosclerosis pathological bone remodeling foci are found exclusively in the otic capsule. We believe the pathogenesis of otosclerosis is linked to the u nique bony dynamics of perilabyrinthine bone and cellular voids may represent a starting point for otosclerotic remodeling. This study aims to identify and characterize cellular voids of the human otic capsule. This would allow future cellular void quantification and comparison of void and otosclerotic distribution to further elucidate the yet unknown pathogenesis of otosclerosis. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Clinical efficacy and safety of MP-AzeFlu for the treatment of allergic rhinitis: a meta-analysis

xlomafota13 shared this article with you from Inoreader Clinical efficacy and safety of MP-AzeFlu for the treatment of allergic rhinitis: a meta-analysis Via http://link.springer.com/journal/405,European archives of oto-rhino-laryngology,The European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino Eur Arch Otorhinolaryngol. 2021 Aug 20. doi: 10.1007/s00405-021-07048-1. Online ahead of print. ABSTRACT OBJECTIVE: MP-AzeFlu is a novel option for therapy of allergic rhinitis (AR). The purpose of our study was to assess the safety and efficacy of MP-AzeFlu for the treatment of allergic rhinitis, compared to placebo and azelastine monotherapy. METHODS: The PubMed, MEDLINE, EMBASE and Cochrane databases were comprehensively searched for all published randomized controlled trials (RCTs) of using MP-AzeFlu nasal spray on July 26, 2019. In these studies, we selected patients with clinical symptom scores. The heterogeneity of the included studies was assessed by I2. RESULTS: Among the 336 citations retrieved, 6 articles with over 6000 patients were finally included in the meta-analysis. The results of meta-analysis revealed that MP-AzeFlu was superior to placebo ( - 2.43 [95%CI, - 2.73 to - 2.14], P < 0.00001) and azelastine ( - 1.27 [95% CI, - 1.57 to - 0.97], P < 0.00001) in reflective total nasal symptom score. In the MP-AzeFlu group, the instantaneous total nasal symptom score ( - 2.56 [95% CI, - 3.02 to - 2.10], P < 0.00001) and the reflective total ocular symptom score ( - 1.22 [95% CI, - 1.57 to - 0.87], P < 0.00001) were significantly reduced compared to the placebo group. CONCLUSION: MP-AzeFlu is as safe and mild as placebo and azelastine, which also is associated with symptom relief and the improvement of quality of life in AR patients. MP-AzeFlu can provide better clinical benefits than two currently available first-line intranasal therapies. It is an ideal therapy for AR patients. PMID:34415405 | DOI:10.1007/s00405-021-07048-1 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Diagnostic performance of nasal cytology

xlomafota13 shared this article with you from Inoreader Diagnostic performance of nasal cytology Via http://link.springer.com/journal/405,European archives of oto-rhino-laryngology,The European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino Eur Arch Otorhinolaryngol. 2021 Aug 19. doi: 10.1007/s00405-021-07044-5. Online ahead of print. ABSTRACT PURPOSE: Nasal pathologies are characterized by a symptomatology that hardly allows to distinguish allergic rhinitis (AR), non-allergic rhinitis (NAR), and chronic rhinosinusitis (CRS). Nasal cytology (NC) has shown increasing importance in helping the clinician to differentiate the various phenotypes of rhinitis. NC allows us to evaluate nasal cellularity by distinguishing AR and various types of NAR. The objective of the study is to assess the diagnostic performance of the NC by evaluating its sensitivity, specificity, and predictive value. METHODS: We recruited 387 patients with persistent rhinitis symptoms, and nasal cytology was performed. The rhinocytogram was obtained by reading for fields and the cellular count was made using quantitative and semi-quantitative grading together. RESULTS: Two hundred and fifteen pa tients (55.5%; 38 had acute rhinitis, 24 acute sinusitis, 153 chronic rhinosinusitis) out of 387 referred nasal symptoms. Cytological specimen showed a mean of 94 ± 4% ciliated cells, 29 ± 0.2% mucinous cells, 16 ± 0.1% neutrophils, 11 ± 0.08% eosinophils, 4 ± 0.03 lymphocytes, 4 ± 0.03% mast cells, and 4 ± 0.01% other cells. NC was positive in 271 cases (70%). After revision of medical history, 153 patients (39%) were considered positive for NAR. Test sensibility was 100% (95% CI 97-100), specificity was 49.6% (95% CI 43-56%). Positive predictive value (PPV) was 56% (95% CI 50-62%), and negative predictive value (NPV) was 100% (95% CI 96-100%). The positive likelihood ratio was 1.98 (95% CI 1.75-2.25). Accuracy of the test was 69.5% (95% CI 64.6-74.0%). CONCLUSION: Our data showed ability to identify the true-positive patients with NAR but a low ability to identify the true-negative patients, with a global accuracy of 69.5%. PMID:34414469 | DOI:10.1007/s00405-021-07044-5 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.