Expression of spindle assembly checkpoint proteins BubR1 and Mad2 expression as potential biomarkers of malignant transformation of oral leukoplakia: an observational cohort study

xlomafota13 shared this article with you from Inoreader Expression of spindle assembly checkpoint proteins BubR1 and Mad2 expression as potential biomarkers of malignant transformation of oral leukoplakia: an observational cohort study Via pubmed: "med oral patol oral... Med Oral Patol Oral Cir Bucal. 2021 Oct 27:24511. doi: 10.4317/medoral.24511. Online ahead of print. ABSTRACT BACKGROUND: The Spindle Assembly Checkpoint (SAC) is a surveillance mechanism essential to ensure the accuracy of chromosome segregation during mitosis. Our aim was to evaluate the expression of SAC proteins in oral carcinogenesis, and to assess their potential in predicting malignant transformation of oral leukoplakia. MATERIAL AND METHODS: We analysed the immunoexpression of BubR1, Mad2, Bub3, and Spindly proteins in 64 oral biopsies from 52 oral leukoplakias and 12 normal tissues. Univariate and multivariate analysis were performed to evaluate predictive factors for malignant transformation (MT). RESULTS: We observed that BubR1 and Mad2 were more highly expressed in high dysplasia grade lesions than in low grade or normal tissues (P<0.05). High expression of Spindly was significantly correlated with a high Ki- 67 score (P=0.004). Six (11.5%) oral leukoplakias underwent malignant transformation. In univariate analysis, the binary dysplasia grade (high grade) (P<0.001) was associated with a higher risk of malignant transformation-free survival (MTFS) as well as high BubR1 (P<0.001) and high Mad2 (P=0.013) expression. In multivariate analysis, high expression of BubR1 and Mad2 when combined showed an increased risk for malignant transformation (P=0.013; HR of 4.6, 95% CI of 1.4-15.1). CONCLUSIONS: Our findings reveal that BubR1 and Mad2 were associated with an increased risk for malignant transformation independently of histological grade and could be potential and useful predictive risk markers of malignant transformation in oral leukoplakias. PMID:34704983 | DOI:10.4317/medoral.24511 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Counselling toward reducing alcohol use, knowledge about its morbidity and personal consumption among students of medical and dental courses in north-western Spain

xlomafota13 shared this article with you from Inoreader Counselling toward reducing alcohol use, knowledge about its morbidity and personal consumption among students of medical and dental courses in north-western Spain Via pubmed: "med oral patol oral... Med Oral Patol Oral Cir Bucal. 2021 Oct 27:24950. doi: 10.4317/medoral.24950. Online ahead of print. ABSTRACT BACKGROUND: Alcohol use disorder (AUD) is directly linked to high-risk consumption. Healthcare students have a crucial role to play in its prevention and management. The aim of this study is to analyse alcohol consumption, as well as to consider the knowledge and attitudes regarding morbidity, and the stage of change when providing assistance to quit AUD. MATERIAL AND METHODS: A cross-sectional study was conducted among Dentistry and Medical students using specific and validated questionnaires in an anonymous and voluntary way. Initially, 925 students were invited to participate, of them 500 were reached. RESULTS: Among them 85.9% suffered from AUD of whom 75% were women (p<0.001), and it was considered that the female gender constituted an independent risk factor (OR=2.63, CI 95% 1.55-4.45, p<0.001). The majo rity of the participants did not achieve the pass mark, nonetheless, the results showed improved levels of knowledge among participants in the latter years of their studies (p<0.001). Dental students demonstrated greater shortcomings in terms of their knowledge of general pathology, whereas the medical students' knowledge of oral pathologies proved worse (p<0.001). Most of students believed that identifying cases of AUD-affected patients falls within their competence, nonetheless, they believed that they do not have the necessary competencies. Among participants 58.2% were in a stage of change regarding AUD attitudes. CONCLUSIONS: The majority of respondents presented AUD. In general, the participants' knowledge about alcohol was low. Reviewing the syllabuses and evaluating the implementation of gender-differentiated training programmes in both degrees would be considered necessary. PMID:34704978 | DOI:10.4317/medoral.24950 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Thyroid function post laryngectomy and hemithyroidectomy ‐ do all laryngectomy patients need thyroid replacement?

xlomafota13 shared this article with you from Inoreader Thyroid function post laryngectomy and hemithyroidectomy ‐ do all laryngectomy patients need thyroid replacement? Via Clinical Otolaryngology Abstract Hypothyroidism is a common complication following laryngectomy with hemithyroidectomy, with a varying incidence between 44-89% amongst this group of patients 1–7. It is often underrecognized due to the insidious onset of thyroid dysfunction and relatively non-specific nature of hypothyroid symptoms. Hypothyroidism may be detrimental to wound healing and be associated with an increased risk of fistula formation, particularly in salvage cases 4,6 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Association between hypertension requiring medication and 30‐day outcomes in head and neck microvascular surgery

xlomafota13 shared this article with you from Inoreader Association between hypertension requiring medication and 30‐day outcomes in head and neck microvascular surgery Via Head & Neck Abstract Background Hypertension has been shown to be both a protective factor and a risk factor for complications in head and neck reconstructive surgery. Methods Retrospective analysis of microvascular free tissue transfer patients using the National Surgical Quality Improvement Program database. Results Hypertensive patients (n = 1598; 46.9%) had a significantly higher rate of complications, including pneumonia (p < 0.001), myocardial infarction (p = 0.003), and intra/post-operative transfusion (p < 0.001). In a multivariable model, hypertension was associated with returning to the operating room (OR = 1.45 [95% CI 1.20, 1.76], p < 0.001), post-operative medical complications (OR = 1.53 [95% CI 1.24, 1.90], p < 0.001), and surgical complications (OR = 1.17 [95% CI 1.00, 1.37], p = 0.047). However, no difference in 30-day readmission was found (p > 0.05). Conclusions Hypertension is a modifiable risk factor for post-operative complications in head and neck free tissue transfer, in which prospective studies are required to establish causation. This study may serve as an impetus for proactive recommendations to manage hypertension before undergoing head and neck microvascular surgery. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Lnc‐MEG3 inhibits invasion, migration, and epithelial– mesenchymal transition of nasopharyngeal carcinoma cells by regulating sequestosome 1

xlomafota13 shared this article with you from Inoreader Lnc‐MEG3 inhibits invasion, migration, and epithelial– mesenchymal transition of nasopharyngeal carcinoma cells by regulating sequestosome 1 Via Head & Neck Abstract Background Long non-coding RNAs regulate malignant behaviors of nasopharyngeal carcinoma (NPC). We aim to investigate the roles and mechanisms of long non-coding RNA maternally expressed gene 3 (lnc-MEG3) in NPC. Methods The expression levels of lnc-MEG3 and sequestosome 1 (SQSTM1) in NPC tissues and cell lines were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell invasion and migration abilities were evaluated using transwell and wound healing assays, respectively. Results Downregulated lnc-MEG3 expression and upregulated SQSTM1 expression were found in NPC tissues and cells. Overexpression of lnc-MEG3 inhibited invasion, migration, and epithelial–mesenchymal transition in NPC cells. Overexpression of lnc-MEG3 reduced the expression level of SQSTM1, and SQSTM1 expression was inversely correlated with lnc-MEG3 level in NPC tissues. Besides, overexpression of SQSTM1 reversed the effects of lnc-MEG3 overexpression. Moreover, knockdown of lnc-MEG3 enhanced NPC progression while its effects were eased by SQSTM1 silence. Conclusion Lnc-MEG3 inhibits malignant behaviors by regulating SQSTM1 expression. It may serve as a therapeutic target to treat NPC. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Effects of Age on Delayed Facial Palsy After Otologic Surgery: A Systematic Review and Meta-Analysis

xlomafota13 shared this article with you from Inoreader Effects of Age on Delayed Facial Palsy After Otologic Surgery: A Systematic Review and Meta-Analysis Via otol rhinol Ann Otol Rhinol Laryngol. 2021 Oct 27:34894211053966. doi: 10.1177/00034894211053966. Online ahead of print. ABSTRACT OBJECTIVES: To report 4 cases of delayed facial palsy (DFP) after pediatric middle ear (ME) surgery and systematically review and analyze the associated literature to evaluate the effects of age on DFP etiology, management, and prognosis. METHODS: Systematic review of PubMed, Cochrane Library, and Embase for articles related to DFP after cochlear implan tation (CI) was performed. These articles were assessed for level of evidence, methodological limitations, and number of cases. Meta-analysis was performed to assess the effects of age on DFP incidence. Furthermore, a comprehensive list of all pediatric DFP cases after otologic surgery was assembled through a multi-institutional retrospective review and systematic review of the literature. RESULTS: Twenty-nine articles fit the criteria for inclusion in the meta-analysis. The incidence of DFP after CI was 0.23% and 1.01% for pediatric and adult cases, respectively. This difference was statistically significant (P < .001, odds ratio 4.36). Twenty-three cases, adding to the 4 presented herein, were suitable for a comprehensive list. The mean age was 6.9 years. Average postoperative day of paresis onset was 5.4, with an average maximum House-Brackmann grade of 3.5. All patients obtained full facial recovery after an average of 23.5 days. CONCLUSIONS: The systematic review demonstrates that DFP after pediatric CI is rare and occurs at a significantly lower rate than in adults, further supporting the viral reactivation hypothesis of DFP. The prognosis for pediatric DFP after otologic surgery is excellent, with a high rate of full recovery in a short time frame. However, steroid administration can be considered. LEVEL OF EVIDENCE: IIa. PMID:34706584 | DOI:10.1177/00034894211053966 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Adenoid Cystic Carcinoma of the Gingiva: A Case Report and Literature Review

xlomafota13 shared this article with you from Inoreader Adenoid Cystic Carcinoma of the Gingiva: A Case Report and Literature Review Via otol rhinol Ann Otol Rhinol Laryngol. 2021 Oct 27:34894211055591. doi: 10.1177/00034894211055591. Online ahead of print. ABSTRACT OBJECTIVES: Adenoid cystic carcinoma (ACC) is a commonly encountered salivary gland malignancy. However, it rarely occurs in the gingiva, an area generally thought to be devoid of minor salivary glands. We present a case occurring in this unusual site and review other reported cases. METHODS: A 56 year-old male presented with a right-sided mandibular to othache for 1 year and underwent dental extraction. Due to persistent pain, follow up examination revealed a large gingival lesion. A biopsy was positive for adenoid cystic carcinoma. RESULTS: The patient underwent a complete right segmental mandibulectomy and was reconstructed with a fibular osteocutaneous free flap. Three months postoperatively, during the planning for adjuvant radiation therapy, the patient developed pain in the left mandible. Imaging revealed extensive involvement of the left native mandible. Deep bone biopsies in several areas of the left mandible revealed ACC. He then underwent a complete left hemi-mandibulectomy and reconstruction with a fibular osteocutaneous free flap. Tensor fascia lata suspension slings were placed due to concern for an open mouth deformity attributable to disruption of bilateral masticator slings. He will undergo adjuvant radiation therapy. Our review of the literature revealed 50 cases of gingival ACC published since 1972. Disease recurrence and distant metastases were noted in several patients, occurring at the latest after 30 years follow-up. CONCLUSIONS: Given its indolent behavior, high proclivity for late recurrence and metastasis, and overall infrequency, ACC represents a pathology that requires early diagnosis and comprehensive long-term surveillance. While ACC is well described in oral cavity sites with high densities of minor salivary glands, it is not commonly seen in the gingiva. As such, gingival ACC may display a unique biological and/or clinical character. We offer the first literature review of this rare entity. PMID:34706573 | DOI:10.1177/00034894211055591 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.