Sex differences of human corpus callosum revealed by polar coordinate system: magnetic resonance imaging study
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 6 δευτερόλεπτα
Sex differences of human corpus callosum revealed by polar coordinate system: magnetic resonance imaging study: *Background:* Evaluation of morphological and size changes related to various pathological conditions of the corpus callosum (CC) requires the data about sex dimorphism of the CC. The purpose of our study is to define potential morphological sex differences of the CC by the use of polar coordinate system as a system of measurements. *Materials and methods:* After division of the CC into three equal segments by the use of polar coordinate system, we investigated the length... περισσότερα »
Immunophilin ligands in peripheral nerve regeneration
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 6 δευτερόλεπτα
Immunophilin ligands in peripheral nerve regeneration: Immunophilins are a family of receptors for immunosuppressive drugs like cyclosporin A, FK506, rapamycin and their non-immunosuppressive analogues, which are collectively referred to as immunophilin ligands. The present study aimed to review neuroprotective and neuroregenerative actions of immunophilin ligands on peripheral nerve injuries. The special emphasis was given to well-known immunosuppressive drug FK506. Its historical background, administration routes, dosages, neuroregenerative, neuroprotective actions, therapeutic ...περισσότερα »
Hematopoietic stem cell transplantation for cutaneous T-cell lymphoma
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 7 δευτερόλεπτα
Hematopoietic stem cell transplantation for cutaneous T-cell lymphoma: Summary of 11 cases from two facilities in Japan and Brazil: Abstract Some patients with cutaneous T-cell lymphoma (CTCL) show a miserable clinical course and the only option that can induce long-term remission for advanced CTCL may be hematopoietic stem cell transplantation (HSCT). So far, studies on HSCT for CTCL patients have been limited. In this study, we summarized 11 cases with CTCL treated with HSCT, including nine cases in Japan and two cases in Brazil. The patients were five cases with mycosis fungoide...περισσότερα »
Evaluation of melanocytic pigmented skin lesions: The 4-point checklist
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 7 δευτερόλεπτα
CASH algorithm versus 3-point checklist and its modified version in evaluation of melanocytic pigmented skin lesions: The 4-point checklist: Abstract Dermoscopy, in expert hands, increases accuracy, sensitivity and specificity in diagnosis of pigmented skin lesions of a single operator, compared with clinical examination. Simplified algorithmic methods have been developed to help less expert dermoscopists in diagnosis of melanocytic lesions. This study included 125 melanocytic skin lesions divided into melanocytic nevi, dysplastic nevi and thin melanomas (<1 mm). We compared the 3-... περισσότερα »
An Additional Option for Split-Thickness Skin Graft Donors: The Previous Free Flap Sites
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 7 δευτερόλεπτα
An Additional Option for Split-Thickness Skin Graft Donors: The Previous Free Flap Sites: Abstract: Free flap reconstruction is the best choice for soft-tissue defect. However, there are often accompanying problems such as partial flap loss, donor-site skin problems, and loss of previous skin grafts surrounding the flap site. This is especially true when dealing with multiple trauma, complex defects, and large skin flaps. Because of the simplicity of the procedure involved, split-thickness skin grafts are usually used for reconstructing skin and soft-tissue defects. These are also a... περισσότερα »
Blepharoptosis Reoperation With Combining Excision of Tarsus and Levator Muscle
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 9 δευτερόλεπτα
Blepharoptosis Reoperation With Combining Excision of Tarsus and Levator Muscle: Abstract: Although various techniques have been reported to correct blepharoptosis, undercorrection is still a commonly occurring complication and needs further revision procedure. Scar formation and distorted anatomy make reoperation more difficult than the primary surgery. However, tarsus is usually fully maintained in the first correction process, and this study combined tarsus resection with levator resection. The resected tarsus can offset the amount of levator excised, which makes this technique ap... περισσότερα »
Surgical Treatment of Angiosarcoma of the Scalp With Superficial Parotidectomy
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 11 δευτερόλεπτα
Surgical Treatment of Angiosarcoma of the Scalp With Superficial Parotidectomy
Prevention of Frey Syndrome With Temporal Fascia Flap in Parotidectomy
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 11 δευτερόλεπτα
Prevention of Frey Syndrome With Temporal Fascia Flap in Parotidectomy: Abstract: The objective of this study was to evaluate a total parotidectomy performed through a face-lift incision integrated with a temporal fascia flap. We have accomplished a group of 40 cases of total parotidectomy from July 2008 to May 2013. Twenty-two cases accepted a modified performance which combined rhytidectomy incision with temporal fascia flap. The other 18 cases were fulfilled by Blair incision and no reconstruction of parotid bed as control. The patients were followed up every 6 months. In the int...περισσότερα »
High-Volume Hydrodissection
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 13 δευτερόλεπτα
High-Volume Hydrodissection: Assessment of Deep Inferior Epigastric Perforator Flap Perfusion Using Laser Doppler: [image: image]Abstract: High-volume hydrodissection of intramuscular perforators of deep inferior epigastric perforator (DIEP) flaps in Sprague-Dawley rats has previously demonstrated a significant reduction in dissection time while simultaneously increasing the safety of dissection. However, increasing volumes of fluid injected into the closed rectus compartment may have adverse consequences on perfusion to the overlying flap. A prospective experimental animal study wa... περισσότερα »
Paramedian Forehead Flap in 2 Stages and 3 Stages : In nasal reconstruction, the paramedian forehead flap is traditionally performed in 2 stages. To minimize the risk of flap necrosis
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 14 δευτερόλεπτα
A Cohort Study of Paramedian Forehead Flap in 2 Stages (87 Flaps) and 3 Stages (100 Flaps): [image: image]Background: In nasal reconstruction, the paramedian forehead flap is traditionally performed in 2 stages. To minimize the risk of flap necrosis, Millard described a 3-stage technique in a series of 5 cases in 1974. In this technique, an intermediate step of flap thinning is performed after flap transfer and before pedicle division. In this article, we compare the 2- and 3-stage techniques of paramedian forehead flaps for nasal reconstruction to determine the type and prevalence ... περισσότερα »
Stromal Vascular Fraction Separation at the Point of Care
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 14 δευτερόλεπτα
Clinical Safety of Stromal Vascular Fraction Separation at the Point of Care: Introduction: Pluripotential cells in adipose tissue may be important in long-term volume retention and regenerative effects of fat grafting. Unfortunately, graft harvest with lipoaspiration significantly depletes the population of stromal vascular cells, which includes adipose stem cells. Stromal vascular fraction (SVF) cells may be isolated from excess lipoaspirate at the point of care and used to replenish fat grafts, a technique termed cell-assisted lipotransfer (CAL). Preclinical and clinical eviden... περισσότερα »
Intraoperative Core Temperature in Free Tissue Transfer
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 15 δευτερόλεπτα
The Clinical Role of Intraoperative Core Temperature in Free Tissue Transfer: [image: image] Background: Lengthy microvascular procedures carry hypothermia risk, yet limited published data evaluate the overall impact of core temperature on patient and flap morbidity. Although hypothermia may contribute to complications, warming measures are challenged by conflicting reports of intraoperative hypothermia improving anastomotic patency. Methods: A retrospective review included all free flaps performed by plastic surgeons at an academic medical center from December 2005 to December 201...περισσότερα »
Intraoperative Flap Evaluation
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 15 δευτερόλεπτα
An Analysis of Current Techniques Used for Intraoperative Flap Evaluation: Background: Over the last decade, microsurgeons have used a greater variety of more complex flaps. At the same time, microsurgeons have also become more interested in technology, such as indocyanine green (ICG) angiography, dynamic infrared thermography (DIRT), and photospectrometry, for preoperative planning and postoperative monitoring. These technologies are now migrating into the operating room, and are used to optimize flap design and to identify areas of hypoperfusion or problems with the anastomoses. A... περισσότερα »
The use of shampoos or soaps containing the anti-fungal agent miconazole nitrate (MCZ)
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 15 δευτερόλεπτα
A study of the suppression of body odour in elderly subjects by anti-fungal agents: Abstract Objective The suppression of body odour following the use of shampoos or soaps containing the anti-fungal agent miconazole nitrate (MCZ) has been recognised anecdotally. To determine whether MCZ could play a role in the suppression of body odour through inhibiting squalene oxidation. Methods A prospective study recruited 54 elderly subjects residing in a nursing facility who needed bathing assistance. Subjects bathed with three types of body soap over a six-week study period (regular soap, s...περισσότερα »
Water-soluble whitening ingredients and adenosine
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 20 δευτερόλεπτα
Simultaneous determination of water-soluble whitening ingredients and adenosine in different cosmetic formulations by high-performance liquid chromatography coupled with photodiode array detection: Abstract Objective The Korean Cosmetic Act regulates the use of functional cosmetics or cosmeceuticals) by the law. Four functional cosmetic groups, whitening, anti-wrinkle, UV protection, and combination of whitening and anti-wrinkle, were categorized according to the Korean Cosmetic Act and Functional Cosmetics Codex. In this study, high-performance liquid chromatography (HPLC) coupled...περισσότερα »
Lymph node density predicts lung metastases in oral squamous cell carcinoma
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 21 δευτερόλεπτα
Lymph node density predicts lung metastases in oral squamous cell carcinoma Abstract The association between lymph node density and survival free of lung metastases in oral squamous cell carcinoma (SCC), has not been investigated so far to our knowledge. Lymph node density ≧ 0.07 has been reported by a multicentre international study to be a significant predictor of shorter survival in patients with oral SCC who have invaded nodes. We investigated whether a lymph node density of ≧ 0.07 correlates with shorter overall survival, survival free of distant metastases, and survival free... περισσότερα »
The viability of microvascularised bone grafts after mandibular reconstruction : New 3-D SPECT images
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 21 δευτερόλεπτα
New 3-dimensional single photon emission computed tomographic images in the assessment of the viability of microvascularised bone grafts after mandibular reconstruction: Reconstruction of mandibular defects is one of the most difficult procedures in maxillofacial surgery. Scapular, iliac crest, and fibular grafts are preferred,1 but lack of viability is common and leads to necrosis, resorption of bone, and a poor outcome. The critical period for the diagnosis of a failed graft is the first two weeks after transplantation, and bone scintigraphy or single photon-emission computed tomo... περισσότερα »
Osteochondromas of the mandibular condyle
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 21 δευτερόλεπτα
TMJ Condylar Osteochondroma: Complete Condylectomy and Joint Replacement vs. Low Condylectomy and Joint Preservation via Oral and Maxillofacial Surgery Publication date: Available online 2 December 2015 *Source:*Journal of Oral and Maxillofacial Surgery Author(s): Pushkar Mehra, Varun Arya, Charles Henry PurposeRecommended treatment for patients with osteochondromas of the mandibular condyle is to perform complete condylectomy with joint replacement. Low condylectomy with joint preservation has been recently been proposed as a treatment option. This study compares the outcomes of both ... περισσότερα »
The dentoalveolar effects of slow and rapid maxillary expansion in complete bilateral cleft lip and palate patients
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 21 δευτερόλεπτα
Analysis of the dentoalveolar effects of slow and rapid maxillary expansion in complete bilateral cleft lip and palate patients: a randomized clinical trial: Abstract ObjectivesThe purpose of this study was to compare the dentoalveolar effects of slow (SME) and rapid (RME) maxillary expansions in patients with complete bilateral cleft lip and palate (BCLP). Material and methodsA sample of 50 patients with BCLP and maxillary arch constriction was randomly and equally allocated into two groups. Group SME comprised patients (mean age of 8.8 years) treated with quad-helix applianc... περισσότερα »
Dermoid Cyst of Submental Region Mimicking Pilomatricoma
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 24 δευτερόλεπτα
Dermoid Cyst of Submental Region Mimicking Pilomatricoma: AbstractDiagnosis of midline neck swellings may give a myriad of options, ranging from ranula, thyroglossal duct cyst, cystic hygroma, cystic lymphangioma, epidermoid, dermoid and teratoid cysts to benign or malignant neoplasms of the mucosa or salivary glands and also rare skin appendage tumours like pilomatricoma or pilomatrix carcinoma. Various pitfalls are encountered in diagnosing these midline swellings as most of the features are overlapping. Careful history and examination have to be complemented with other diagnosti...περισσότερα »
L’utilisation de l’acide désoxycholique (ATX-101) en médecine esthétique.ATX-101 is a synthetic derivative from deoxycholic acid, which leads to destruction of adipocytes and to a skin retraction
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 26 δευτερόλεπτα
: un traitement prometteur via Revue de Stomatologie, de Chirurgie Maxillo-faciale et de Chirurgie Orale Publication date: Available online 1 December 2015 *Source:*Revue de Stomatologie, de Chirurgie Maxillo-faciale et de Chirurgie Orale Author(s): B. Hersant, A. Calmon, J.P. Meningaud La technique dite de lipolyse par injection d’acide désoxycholique est une méthode non invasive, alternative à la lipoaspiration, sans anesthésie et sans cicatrice qui consiste en une injection directement dans le tissus adipeux sous-cutané. L’ATX-101 est un dérivé synthétique de l’acide désoxycholiqu...περισσότερα »
Bruxism Episode Index (BEI)
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 26 δευτερόλεπτα
Beyond Bruxism – Bruxism Episode Index (BEI): One of the most common dental diseases is bruxism, which research suggests may affect nearly one in every three patients. It’s time to start paying attention to bruxism for a number of reasons, especially because your treatment may be exacerbating other medical conditions. The most common treatment for bruxism is to create a bruxism [image: sleepy bruxism] appliance for the patient. Many dentists fall in love with one type of bruxism appliance and they use it for all of their bruxism/ temporomandibular disorder patients. The patient go... περισσότερα »
In-laboratory and home diagnosis of sleep apnea using a cordless portable acoustic device
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 26 δευτερόλεπτα
Comparison of via Sleep Medicine Publication date: Available online 2 December 2015 *Source:*Sleep Medicine Author(s): Hisham Alshaer, Geoff R. Fernie, Wen-Hou Tseng, T. Douglas Bradley Background and Objectives:Sleep Apnea (SA) is a common, serious, but underdiagnosed condition. There is a need for more accessible and economic means of diagnosing SA in the home. The objective of this study was to test the validity of a cordless acoustic portable device (BresoDx™) for home diagnosis of SA compared to standard polysomnography (PSG).Methods135 subjects underwent full overnight PSG and ... περισσότερα »
Oscillatory cardiopulmonary coupling in patients with obstructive sleep apnea hypopnea syndrome by impedance cardiogram
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 26 δευτερόλεπτα
Evaluating the clinical value of via Sleep Medicine Publication date: Available online 2 December 2015 *Source:*Sleep Medicine Author(s): Xue Chen, Ting Zhou, Dongfang Li, Cheng Zhang, Peng Jia, Jing Ma, Jue Zhang, Guangfa Wang, Jing Fang ObjectivesFor patients with obstructive sleep apnea hypopnea syndrome (OSAHS), chronic inflammation and hemodynamic oscillations caused by respiratory events contribute to cardiovascular disease (CVD). In this study, a physiological marker named oscillatory coupling factor (OCF) exacted from cardiac output (CO) was introduced. This study aimed to ev... περισσότερα »
Treatment of sleep apnea in chronic heart failure patients with auto-servo ventilation
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 26 δευτερόλεπτα
improves sleep fragmentation: a randomized controlled trial Publication date: January 2016 *Source:*Sleep Medicine, Volume 17 Author(s): Andrea Hetzenecker, Pierre Escourrou, Samuel T. Kuna, Frederic Series, Keir Lewis, Christoph Birner, Michael Pfeifer, Michael Arzt Background Impaired sleep efficiency is independently associated with worse prognosis in patients with chronic heart failure (CHF). Therefore, a test was conducted on whether auto-servo ventilation (ASV, biphasic positive airway pressure [BiPAP]–ASV, Philips Respironics) reduces sleep fragmentation and improves sleep ef... περισσότερα »
Pregnancy and estrogen enhance neural progenitor-cell proliferation in the vomeronasal sensory epithelium.
Alexandros G. SfakianakisστοΓεύση και ΌσφρησηΠριν από 28 δευτερόλεπτα
Pregnancy and estrogen enhance neural progenitor-cell proliferation in the vomeronasal sensory epithelium.: *Pregnancy and estrogen enhance neural progenitor-cell proliferation in the vomeronasal sensory epithelium.* BMC Biol. 2015;13:104 Authors: Oboti L, Ibarra-Soria X, Pérez-Gómez A, Schmid A, Pyrski M, Paschek N, Kircher S, Logan DW, Leinders-Zufall T, Zufall F, Chamero P Abstract BACKGROUND: The hormonal state during the estrus cycle or pregnancy produces alterations on female olfactory perception that are accompanied by specific maternal behaviors, but it is unclear how sex...περισσότερα »
Sleep Medicine
Alexandros G. SfakianakisστοΥπνική Άπνοια και Διαταραχές ΎπνουΠριν από 34 δευτερόλεπτα
Long-term adherence to continuous positive airway pressure therapy in non-sleepy sleep apnea patients Publication date: January 2016 *Source:*Sleep Medicine, Volume 17 Author(s): Francisco Campos-Rodriguez, Montserrat Martinez-Alonso, Manuel Sanchez-de-la-Torre, Ferran Barbe Objective The effectiveness of continuous positive airway pressure (CPAP) therapy greatly depends on consistent use. However, data regarding adherence in non-sleepy obstructive sleep apnea (OSA) patients are scarce. The aim of this study was to assess long-term adherence and predictors of CPAP compliance in a la... περισσότερα »
Evaluation of antimicrobial properties of cork
Alexandros G. SfakianakisστοΛοίμωξηΠριν από 37 δευτερόλεπτα
Cork presents a range of diverse and versatile properties making this material suitable for several and extremely diverse industrial applications. Despite the wide uses of cork, its antimicrobial properties and potential applications have deserved little attention from industry and the scientific community. Thus, the main purpose of this work was the evaluation of the antibacterial properties of cork, by comparison with commercially available antimicrobial materials (EVA copolymer and a currently used antimicrobial commercial additive (ACA)), following the previous development and... περισσότερα »
Restraining of Reactive Oxygen Species (ROS) Promotes Invasion of Listeria monocytogenes into Glia Cells
Alexandros G. SfakianakisστοΛοίμωξηΠριν από 37 δευτερόλεπτα
*Listeria monocytogenes* is a foodborne pathogen that could cause severe infection in the central nervous system of humans and animals. However, the molecular mechanism of the pathogenesis is not fundamentally assessed. This study aimed to analyze the role of reactive oxygen species (ROS) in *L. monocytogenes* during its invasion into glia cells. The ROS level in *L. monocytogenes* was manipulated using NAD(P)H oxidase inhibitor diphenyleneiodonium chloride (DPI) and ROS scavenger N-acetyl cysteine (NAC). Results showed that the invasiveness of *L. monocytogenes* was elevated when... περισσότερα »
Evaluation of antimicrobial properties of cork
Alexandros G. SfakianakisστοΛοίμωξηΠριν από 37 δευτερόλεπτα
Cork presents a range of diverse and versatile properties making this material suitable for several and extremely diverse industrial applications. Despite the wide uses of cork, its antimicrobial properties and potential applications have deserved little attention from industry and the scientific community. Thus, the main purpose of this work was the evaluation of the antibacterial properties of cork, by comparison with commercially available antimicrobial materials (EVA copolymer and a currently used antimicrobial commercial additive (ACA)), following the previous development and... περισσότερα »
Restraining of Reactive Oxygen Species (ROS) Promotes Invasion of Listeria monocytogenes into Glia Cells
Alexandros G. SfakianakisστοΛοίμωξηΠριν από 37 δευτερόλεπτα
*Listeria monocytogenes* is a foodborne pathogen that could cause severe infection in the central nervous system of humans and animals. However, the molecular mechanism of the pathogenesis is not fundamentally assessed. This study aimed to analyze the role of reactive oxygen species (ROS) in *L. monocytogenes* during its invasion into glia cells. The ROS level in *L. monocytogenes* was manipulated using NAD(P)H oxidase inhibitor diphenyleneiodonium chloride (DPI) and ROS scavenger N-acetyl cysteine (NAC). Results showed that the invasiveness of *L. monocytogenes* was elevated when... περισσότερα »
Obstructive sleep apnea cardiac complications and sleepiness in children
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 37 δευτερόλεπτα
Relationship between obstructive sleep apnea cardiac complications and sleepiness in children with Down syndrome Publication date: January 2016 *Source:*Sleep Medicine, Volume 17 Author(s): Sofia Konstantinopoulou, Ignacio E. Tapia, Ji Young Kim, Melissa S. Xanthopoulos, Jerilynn Radcliffe, Meryl S. Cohen, Brian D. Hanna, Mary Pipan, Christopher Cielo, Allison J. Thomas, Babette Zemel, Raouf Amin, Ruth Bradford, Joel Traylor, Justine Shults, Carole L. Marcus Objective/Background Children with Down syndrome (DS) have a high rate of pulmonary hypertension and sleepiness. They also have... περισσότερα »
Primary snoring in children: the effect of adenotonsillectomy
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 37 δευτερόλεπτα
The history of Publication date: January 2016 *Source:*Sleep Medicine, Volume 17 Author(s): Adi Borovich, Yakov Sivan, Michal Greenfeld, Riva Tauman Primary snoring (PS) is considered as the most benign form of sleep-disordered breathing (SDB), and treatment is usually not prescribed. Studies suggest that PS may not be as benign as had formerly been considered. We aimed to investigate the natural history of PS in children with adenotonsillar hypertrophy, and compare those who underwent adenotonsillectomy (AT) with those who did not. Material and methods Children diagnosed with PS b... περισσότερα »
Insomnia and daytime neuropsychological test performance
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 37 δευτερόλεπτα
in older adults Publication date: January 2016 *Source:*Sleep Medicine, Volume 17 Author(s): Audrey Ling, May Li Lim, Xinyi Gwee, Roger C.M. Ho, Simon L. Collinson, Tze-Pin Ng Objectives/Background There is good documentation of the impact of insomnia on daytime cognitive function based on self-reports, but not on neuropsychological test performance. The study investigated the association of difficulty initiating sleep (DIS), difficulty maintaining sleep (DMS), and early morning awakening (EMA) complaints with daytime domain-specific neuropsychological performance in older adults.... περισσότερα »
cpap therapy in non-sleepy sleep apnea patients
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 38 δευτερόλεπτα
Long-term adherence to continuous positive airway pressure therapy in non-sleepy sleep apnea patients Publication date: January 2016 *Source:*Sleep Medicine, Volume 17 Author(s): Francisco Campos-Rodriguez, Montserrat Martinez-Alonso, Manuel Sanchez-de-la-Torre, Ferran Barbe Objective The effectiveness of continuous positive airway pressure (CPAP) therapy greatly depends on consistent use. However, data regarding adherence in non-sleepy obstructive sleep apnea (OSA) patients are scarce. The aim of this study was to assess long-term adherence and predictors of CPAP compliance in a la... περισσότερα »
The Journal of the Acoustical Society of America
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 38 δευτερόλεπτα
Underdetermined reverberant acoustic source separation using weighted full-rank nonnegative tensor models K Effects of voice style, noise level, and acoustic feedback on objective and subjective voice evaluations room acoustic Enhancing cell kill in vitro from hyperthermia through pre-sensitizing with ultrasound-stimulated microbubbles microbubbles The ambient acoustic environment in Laguna San Ignacio, Baja California Sur, Mexico Eschrichtius robustus A two-way coupled mode formalism that satisfies energy conservation for impedance boundaries in underwater acoustics energy conservatio...περισσότερα »
Gait & Posture
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 38 δευτερόλεπτα
The use of force-plate posturography in the assessment of postural instability Publication date: February 2016 *Source:*Gait & Posture, Volume 44 Author(s): Janusz W. Błaszczyk Force-plate posturography is a simple method that is commonly used in the contemporary laboratory and clinic to assess postural control. Despite the obvious advantages and popularity of the method, universal standards for posturographic tests have not been developed thus far: most postural assessments are based on the standard spatiotemporal metrics of the center-of-foot pressure (COP) recorded during qui...περισσότερα »
Force-plate posturography in the assessment of postural instability
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 38 δευτερόλεπτα
The use of Publication date: February 2016 *Source:*Gait & Posture, Volume 44 Author(s): Janusz W. Błaszczyk Force-plate posturography is a simple method that is commonly used in the contemporary laboratory and clinic to assess postural control. Despite the obvious advantages and popularity of the method, universal standards for posturographic tests have not been developed thus far: most postural assessments are based on the standard spatiotemporal metrics of the center-of-foot pressure (COP) recorded during quiet stance. Unfortunately, the standard COP characteristics are stro...περισσότερα »
Motion sickness and sopite syndrome associated with parabolic flights: a case report
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 38 δευτερόλεπτα
Abstract Objective: The purpose of this paper was to provide an extensive description of a case of sopite syndrome, a manifestation of motion sickness scarcely described in the literature. Design: The following questionnaires were used to assess sopite syndrome symptoms pre, during and post parabolic flight; Misery Scale rate, Positive and Negative Affect Scale, the Motion Sickness Assessment Questionnaire and the Epworth Sleepiness Scale. Study sample: Case report of a 35-year old female compared to a control group (n = 26). Results: We describe of sopite syndrome during parabolic fl...περισσότερα »
Pediatric Facial Trauma from Bicycling
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 38 δευτερόλεπτα
The Vicious Cycle: Pediatric Facial Trauma from Bicycling: Objectives Although prevention of head injuries through helmet use is widespread, there has been a paucity of inquiry and publicity regarding the potential for facial injury stemming from cycling. Our objectives included estimating the incidence of emergency department (ED) visits for bicycle-related facial trauma among the pediatric population and detailing injury patterns. Study Design and Setting Analysis of a nationwide database. Methods The National Electronic Injury Surveillance System, offered by the Consumer Produc... περισσότερα »
Revisiting Place-Pitch Match in CI Recipients Using 3D Imaging Analysis
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 38 δευτερόλεπτα
Revisiting Place-Pitch Match in CI Recipients Using 3D Imaging Analysis: Objective: To improve the estimation of the perceived pitch in a single-sided deaf cochlear implant (CI) listener by using accurate 3-dimensional (3D) image analysis of the cochlear electrode positions together with the predicted tonotopical function for humans. Methods: An SSD CI user underwent a Cone-Beam computed tomography (CBCT) scan. Electrode contacts were marked in 3D space in relation to the nearest point on the cochlear lateral wall. Distance to the base of the lateral wall was calculated and plotte...περισσότερα »
Malleus to Stapes Bone Cement Rebridging Ossiculoplasty
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 46 δευτερόλεπτα
Malleus to Stapes Bone Cement Rebridging Ossiculoplasty: Why Dont We Perform Frequently?: Objective: To evaluate the effectiveness of malleus to stapes bone cement rebridging (MS-BCR) for Austin Kartush group A ossicular defects and compare the audiological results with incus interposition (IP) and incus to stapes bone cement rebridging (IS-BCR). Methods: Patients for whom type 2 tympanoplasty had been performed in a tertiary referral center were examined. Revision cases and those with graft failure were excluded. Three treatment groups were IS-BCR, IP, and MS-BCR. Preoperative an...περισσότερα »
Incidence of cutaneous malignant melanoma by socioeconomic status
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 46 δευτερόλεπτα
Incidence of cutaneous malignant melanoma by socioeconomic status in Canada: 1992–2006: Background: There are no nationwide studies documenting changes in cutaneous malignant melanoma incidence or association of incidence with socioeconomic status (SES) in Canada. We sought to determine whether melanoma incidence increased from 1992 to 2006 and if there was an association between SES and melanoma incidence. Additionally, we studied whether there was a correlation between province of residence and melanoma incidence. Methods: Cases from the Canadian Cancer Registry were reviewed. Demog... περισσότερα »
The Neutrophil-Lymphocyte Ratio in Patients with Idiopathic Peripheral Facial Palsy
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 46 δευτερόλεπτα
The Importance of the Neutrophil-Lymphocyte Ratio in Patients with Idiopathic Peripheral Facial Palsy: Objective. The purpose of this study was to investigate whether or not there was a correlation between the neutrophil-to-lymphocyte ratio (NLR) value and the severity of idiopathic peripheral facial palsy (IPFP) and to determine whether or not NLR could be used as an early predictive parameter in the prognosis of IPFP patients. Material and Method. This retrospective study was conducted on 146 patients who were diagnosed with IPFP. The control group comprised 140 patients. Patients...περισσότερα »
IoN- Is Ablative Radio-iodine Necessary for Low Risk Differentiated Thyroid Cancer Patients
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 47 δευτερόλεπτα
IoN- Is Ablative Radio-iodine Necessary for Low Risk Differentiated Thyroid Cancer Patients: *Condition*: Thyroid Cancer *Intervention*: Radiation: I131 1.1 GBq *Sponsors*: University College, London; Cancer Research UK ArmsAssigned InterventionsActive Comparator: Radioactive iodine (RAI) ablation Arm Patients will be randomised to receive Radioactive iodine (RAI) ablation I131 1.1 GBq Radiation: I131 1.1 GBq Radio-iodine Other Name: Sodium iodide capsule No Intervention: No Radioactive iodine (No-RAI) ablation Patients will be randomised to receive No Radioactive iodine (No...περισσότερα »
Dose-finding Study of Metformin With Chemoradiation in Locally Advanced Head and Neck Squamous Cell Carcinoma
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 47 δευτερόλεπτα
Dose-finding Study of Metformin With Chemoradiation in Locally Advanced Head and Neck Squamous Cell Carcinoma: *Condition*: Head and Neck Cancer *Interventions*: Drug: Metformin; Drug: Cisplatin; Radiation: Radiation Therapy *Sponsor*: University of Cincinnati ArmsAssigned InterventionsExperimental: Metformin with Chemoradiation Metformin administered orally daily to start one week prior to Cisplatin and Radiation Therapy. Metformin dose is escalating. Drug: Metformin Escalating doses of 2000mg, 2550 mg and 3000mg. Starting 1 week prior to the initiation of chemoradiation...περισσότερα »
TAA-Specific CTLS for Solid Tumors (TACTASOM)
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 48 δευτερόλεπτα
TAA-Specific CTLS for Solid Tumors (TACTASOM): *Conditions*: Bladder Cancer; Breast Cancer; Colorectal Cancer; Endometrial Cancer; Gastric Cancer; Head and Neck Cancer; HCC; Lung Cancer; Melanoma; Neuroblastoma; Sarcoma (Osteo-, Synovial, Rhabdomyo-, Etc); Pancreatic Cancer; Prostate Cancer; Renal Cell Carcinoma; Thyroid Cancer *Intervention*: Biological: TAA-Specific CTLs *Sponsors*: Baylor College of Medicine; The Methodist Hospital System; Center for Cell and Gene Therapy, Baylor College of Medicine; Texas Children's Hospital This is a ... περισσότερα »
I-124 PET/CT Based Remnant Radioiodine Ablation Decision Concept in Differentiated Thyroid Cancer
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 48 δευτερόλεπτα
I-124 PET/CT Based Remnant Radioiodine Ablation Decision Concept in Differentiated Thyroid Cancer: *Condition*: Differentiated Thyroid Carcinoma *Interventions*: Radiation: Radioiodine I-131; Radiation: I-124 *Sponsors*: University of Wuerzburg; Deutsche Krebshilfe e.V., Bonn (Germany) Thyroid nodules are a common clinical problem. Epidemiologic studies have shown the prevalence of palpable thyroid nodules to be approximately 5% in women and 1% in men living in iodine-sufficient parts of the world and up to 30% in iodine deficient regions, such as Germany. The clinical ... περισσότερα »
MLN8237 and Pazopanib in Combination for Solid Tumors
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 49 δευτερόλεπτα
MLN8237 and Pazopanib in Combination for Solid Tumors: *Conditions*: Malignant Neoplasm of Breast; CNS Malignancy; Malignant Neoplasm of Gastrointestinal Tract; Genitourinary Neoplasms Malignancy and Gender Unspecified; Head and Neck Neoplasms; Melanoma; Malignant Neoplasm of Thorax *Interventions*: Drug: Alisertib; Drug: Pazopanib *Sponsors*: University of Illinois at Chicago; Millennium Pharmaceuticals, Inc. ArmsAssigned InterventionsExperimental: Treated Patients with Solid Tumor Alisertib at the assigned dose (10, 20, 40 or 50 mg) by mouth PO twice a day... περισσότερα »
IoN- Is Ablative Radio-iodine Necessary for Low Risk Differentiated Thyroid Cancer Patients
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 49 δευτερόλεπτα
IoN- Is Ablative Radio-iodine Necessary for Low Risk Differentiated Thyroid Cancer Patients: *Condition*: Thyroid Cancer *Intervention*: Radiation: I131 1.1 GBq *Sponsors*: University College, London; Cancer Research UK ArmsAssigned InterventionsActive Comparator: Radioactive iodine (RAI) ablation Arm Patients will be randomised to receive Radioactive iodine (RAI) ablation I131 1.1 GBq Radiation: I131 1.1 GBq Radio-iodine Other Name: Sodium iodide capsule No Intervention: No Radioactive iodine (No-RAI) ablation Patients will be randomised to receive No Radioactive iodine (No...περισσότερα »
Drug-Induced Sleep Endoscopy for Upper Airway Evaluation in Obstructive Sleep Apnea
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 49 δευτερόλεπτα
Drug-Induced Sleep Endoscopy for Upper Airway Evaluation in Obstructive Sleep Apnea: *Condition*: Sleep Apnea, Obstructive *Intervention*: Drug: Propofol sedation *Sponsors*: University of Southern California; National Institutes of Health (NIH); American Academy of Otolaryngology-Head and Neck Surgery Foundation Prospective, interventional cohort study of drug-induced sleep endoscopy (DISE) to evaluate the upper airway in a cohort of obstructive sleep apnea (OSA) surgical patients. This study has investigated the reliability of this technique, demonstrating moderate-su... περισσότερα »
Outcomes After Esophagectomy With a Focus on Minimally Invasive Esophagectomy and Quality of Life
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 49 δευτερόλεπτα
Outcomes After Esophagectomy With a Focus on Minimally Invasive Esophagectomy and Quality of Life: *Conditions*: Esophagectomy; Esophageal Cancer *Intervention*: Procedure: Questionnaires *Sponsor*: University of Pittsburgh Assess short and long term outcomes after minimally invasive esophagectomy(MIE) compared to open esophagectomy. Measure standard observer derived outcomes such as morbidity, mortality, tumor recurrence and also patient derived outcomes, in particular quality of life (QOL) using the MOS SF36 questionnaire. Evaluate whether the SF36 will accurately refl...περισσότερα »
Esophageal Cancer Risk Registry
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 49 δευτερόλεπτα
Esophageal Cancer Risk Registry: *Conditions*: Esophageal Cancer; Gastroesophageal Reflux Disease; Esophageal Diseases; Hiatal Hernia; Esophageal Achalasia *Interventions*: Procedure: Survey of client health, personal habits, family history; Procedure: Blood specimen for non-DNA and DNA parts; Procedure: Biopsy of esophageal/stomach tissue, lymph nodes, tumor; Procedure: Biopsies from routine clinical surveillance endoscopies *Sponsors*: University of Pittsburgh; National Institutes of Health (NIH) The purpose of this study is to identify markers in the bloo... περισσότερα »
Colorectal Adenocarcinoma Metastasis to the Tongue
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 49 δευτερόλεπτα
Colorectal Adenocarcinoma Metastasis to the Tongue: This case presentation examines a rare clinical entity: colorectal adenocarcinoma (CRC) metastasis to the tongue. CRC is among the least common tumors to metastasize to the oral cavity. Objectives for this case report are to (1) maintain a high index of suspicion for oral cavity tumors representing metastatic disease, (2) consider appropriate surgical and adjunctive interventions, and (3) recognize the significance of identifying the primary tumor via immunohistochemical staining. We present a case of a 57-year-old male with a hist...περισσότερα »
Management of Pediatric Cholesteatoma
Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 49 δευτερόλεπτα
based on presentations, complications, and outcomes via Pediatric Otorhinolaryngology Publication date: Available online 2 December 2015 *Source:*International Journal of Pediatric Otorhinolaryngology Author(s): J.K. McGuire, H. Wasl, T. Harris, G.J. Copley, J.J. Fagan ObjectivesTo highlight important aspects and paradigms in the management of paediatric cholesteatoma in a developing world settingMethodsA retrospective audit was conducted of paediatric cholesteatomas that underwent tympanomastoid surgery between 2008 and 2012 at the Red Cross War Memorial Children's Hospital in Cape To... περισσότερα »
Soil bacterial community responses to warming and grazing in a Tibetan alpine meadow
Alexandros G. SfakianakisστοΛοίμωξηΠριν από 1 ώρα
Warming and grazing significantly affect the structure and function of an alpine meadow ecosystem. Yet, the responses of soil microbes to these disturbances are not well understood. Controlled asymmetrical warming (+1.2/1.7°C during daytime/nighttime) with grazing experiments were conducted to study microbial response to warming, grazing, and their interactions. Significant interactive effects of warming and grazing were observed on soil bacterial α-diversity and composition. Warming only caused significant increase in bacterial α-diversity under no-grazing conditions. Grazing ind...περισσότερα »
Diversification of Broad Host Range Plasmids Correlates with the Presence of Antibiotic Resistance Genes
Alexandros G. SfakianakisστοΛοίμωξηΠριν από 1 ώρα
The IncP-1 subgroup is a recently identified phylogenetic clade within IncP-1 plasmids, which plays an important role in the spread of antibiotic resistance and degradation of xenobiotic pollutants. Here, four IncP-1 plasmids were exogenously captured from a petroleum-contaminated habitat in China and compared phylogenetically and genomically with previously reported IncP-1 and other IncP-1 plasmids. The IncP-1 plasmids can be clearly subdivided into two subclades, designated as -I and -II, based on phylogenetic analysis of backbone proteins TraI and TrfA. This was further support... περισσότερα »
Impact of different diagnostic technologies for MRSA admission screening in hospitals – a decision tree analysis
Alexandros G. SfakianakisστοΛοίμωξηΠριν από 1 ώρα
Background: Hospital infections with multiresistant bacteria, e.g., Methicillin-resistant Staphylococcus aureus (MRSA), cause heavy financial burden worldwide. Rapid and precise identification of MRSA carriage in combination with targeted hygienic management are proven to be effective but incur relevant extra costs. Therefore, health care providers have to decide which MRSA screening strategy and which diagnostic technology should be applied according to economic criteria.AimThe aim of this study was to determine which MRSA admission screening and infection control management stra... περισσότερα »
ASM Santa Hat/Happy Holidays
Alexandros G. SfakianakisστοΛοίμωξηΠριν από 1 ώρα
ASM Santa Hat....Bacteria used: Red portion of hat and letters: Serratia marcescens White hat trim: Staph saprophyticus Large TSA plate grown for 24 hrs at 37 degree's C from Infection via ola Kala on Inoreader http://ift.tt/1O5abcx via IFTTT
Holiday Time, smile!
Alexandros G. SfakianakisστοΛοίμωξηΠριν από 1 ώρα
Holiday time......Bacteria/Yeast used: Hat and letters, Serratia marcescens Hat trim: Staph epidermidis Smile: Environmental yeast isolate Mix of Serratia and Staph for a bit of "snow" from Infection via ola Kala on Inoreader http://ift.tt/1SxXric via IFTTT
TWiM 116: Chewates and coconuts
Alexandros G. SfakianakisστοΛοίμωξηΠριν από 1 ώρα
Hosts: Vincent Racaniello, from Infection via ola Kala on Inoreader http://ift.tt/1SxXr1T via IFTTT
Mismatch repair defects and Lynch syndrome: the role of the basic scientist in the battle against cancer
Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 1 ώρα
Publication date: Available online 2 December 2015 *Source:*DNA Repair Author(s): Christopher D. Heinen We have currently entered a genomic era of cancer research which may soon lead to a genomic era of cancer treatment. Patient DNA sequencing information may lead to a personalized approach to managing an individual's cancer as well as future cancer risk. The success of this approach, however, begins not necessarily in the clinician's office, but rather at the laboratory bench of the basic scientist. The basic scientist plays a critical role since the DNA sequencing information is o...περισσότερα »
Antibody diversification caused by disrupted mismatch repair and promiscuous DNA polymerases
Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 1 ώρα
Publication date: Available online 2 December 2015 *Source:*DNA Repair Author(s): Kimberly J. Zanotti, Patricia J. Gearhart The enzyme activation-induced deaminase (AID) targets the immunoglobulin loci in activated B cells and creates DNA mutations in the antigen-binding variable region and DNA breaks in the switch region through processes known, respectively, as somatic hypermutation and class switch recombination. AID deaminates cytosine to uracil in DNA to create a U:G mismatch. During somatic hypermutation, the MutSα complex binds to the mismatch, and the error-prone DNA polymer... περισσότερα »
The conserved molecular machinery in DNA mismatch repair enzyme structures
Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 1 ώρα
Publication date: Available online 2 December 2015 *Source:*DNA Repair Author(s): Flora S. Groothuizen, Titia K. Sixma The machinery of DNA mismatch repair enzymes is highly conserved in evolution. The process is initiated by recognition of a DNA mismatch, and validated by ATP and the presence of a processivity clamp or a methylation mark. Several events in MMR promote conformational changes that lead to progression of the repair process. Here we discuss functional conformational changes in the MMR proteins and we compare the enzymes to paralogs in other systems. from #Medicine v... περισσότερα »
DNA[NdW1] Mismatch Repair and the DNA Damage Response[NdW2]
Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 1 ώρα
Publication date: Available online 2 December 2015 *Source:*DNA Repair Author(s): Zhongdao Li, Alexander H. Pearlman, Peggy Hsieh This review discusses the role of DNA mismatch repair (MMR) in the DNA damage response (DDR) that triggers cell cycle arrest and, in some cases, apoptosis. Although the focus is on findings from mammalian cells, much has been learned from studies in other organisms including bacteria and yeast [1,2]. MMR promotes a DDR mediated by a key signaling kinase, ATM and Rad3-related (ATR), in response to various types of DNA damage including some encountered in w... περισσότερα »
Mismatch binding, ADP-ATP exchange and intramolecular signaling during mismatch repair
Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 1 ώρα
Publication date: Available online 2 December 2015 *Source:*DNA Repair Author(s): Manju M. Hingorani The focus of this article is on the DNA binding and ATPase activities of the mismatch repair (MMR) protein, MutS—our current understanding of how this protein uses ATP to fuel its actions on DNA and initiate repair via interactions with MutL, the next protein in the pathway. Structure-function and kinetic studies have yielded detailed views of the MutS mechanism of action in MMR. How MutS and MutL work together after mismatch recognition to enable strand-specific nicking, which leads... περισσότερα »
Roles for mismatch repair family proteins in promoting meiotic crossing over
Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 1 ώρα
Publication date: Available online 2 December 2015 *Source:*DNA Repair Author(s): Carol M. Manhart, Eric Alani The mismatch repair (MMR) family complexes Msh4-Msh5 and Mlh1-Mlh3 act with Exo1 and Sgs1-Top3-Rmi1 in a meiotic double strand break repair pathway that results in the asymmetric cleavage of double Holliday junctions (dHJ) to form crossovers. This review discusses how meiotic roles for Msh4-Msh5 and Mlh1-Mlh3 do not fit paradigms established for post-replicative MMR. We also outline models used to explain how these factors promote the formation of meiotic crossovers require... περισσότερα »
Evolution of the methyl directed mismatch repair system in Escherichia coli
Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 1 ώρα
Publication date: Available online 2 December 2015 *Source:*DNA Repair Author(s): Christopher D. Putnam DNA mismatch repair (MMR) repairs mispaired bases in DNA generated by replication errors. MutS or MutS homologs recognize mispairs and coordinate with MutL or MutL homologs to direct excision of the newly synthesized DNA strand. In most organisms, the signal that discriminates between the newly synthesized and template DNA strands has not been definitively identified. In contrast, Escherichia coli and some related gammaproteobacteria use a highly elaborated methyl-directed MMR sys...περισσότερα »
DNA microarray-based solid-phase PCR on copoly (DMA–NAS–MAPS) silicon coated slides: An example of relevant clinical application
Alexandros G. SfakianakisστοΝοσολογίαΠριν από 1 ώρα
[image: S09565663.gif] Publication date: 15 April 2016 *Source:*Biosensors and Bioelectronics, Volume 78 Author(s): Francesco Damin, Silvia Galbiati, Maurizio Ferrari, Marcella Chiari In a previous study we developed a highly sensitive DNA microarray for the detection of common KRAS oncogenic mutations, which has been proven to be highly specific in assigning the correct genotype without any enrichment strategy even in the presence of minority mutated alleles. However, in this approach, the need of a spotter for the deposition of the purified PCR products on the substrates and the p...περισσότερα »
Sensitive detection of Campylobacter jejuni using nanoparticles enhanced QCM sensor
Alexandros G. SfakianakisστοΝοσολογίαΠριν από 1 ώρα
[image: S09565663.gif] Publication date: 15 April 2016 *Source:*Biosensors and Bioelectronics, Volume 78 Author(s): Noor Azlina Masdor, Zeynep Altintas, Ibtisam E. Tothill A quartz crystal microbalance (QCM) sensor platform was used to develop an immunosensor for the detection of food pathogen Campylobacter jejuni. Rabbit polyclonal antibodies and commercially available mouse monoclonal antibodies against C. jejuni were investigated to construct direct, sandwich and gold-nanoparticles (AuNPs) amplified sandwich assays. The performance of the QCM immunosensor developed using sandwich ... περισσότερα »
HIV-1 Tat biosensor: Current development and trends for early detection strategies
Alexandros G. SfakianakisστοΝοσολογίαΠριν από 1 ώρα
[image: S09565663.gif] Publication date: 15 April 2016 *Source:*Biosensors and Bioelectronics, Volume 78 Author(s): M.F. Fatin, A.R. Ruslinda, M.K. Md Arshad, K.K. Tee, R.M. Ayub, U. Hashim, A. Kamarulzaman, Subash C.B. Gopinath Human immunodeficiency virus (HIV) has infected almost 35 million people worldwide. Various tests have been developed to detect the presence of HIV during the early stages of the disease in order to reduce the risk of transmission to other humans. The HIV-1 Tat protein is one of the proteins present in HIV that are released abundantly approximately 2–4 weeks ...περισσότερα »
In-situ assembly of biocompatible core–shell hierarchical nanostructures sensitized immunosensor for microcystin-LR detection
Alexandros G. SfakianakisστοΝοσολογίαΠριν από 1 ώρα
[image: S09565663.gif] Publication date: 15 April 2016 *Source:*Biosensors and Bioelectronics, Volume 78 Author(s): Cuifen Gan, Li Ling, Zuyu He, Hongtao Lei, Yingju Liu Microcystin-LR (MC-LR) is a kind of hepatotoxin which can cause functional and structural disturbances of the liver, accumulate in aquatic organisms and transfer to higher trophic levels, a biocompatible electrochemical immunosensor was constructed to detect MC-LR sensitively and selectively. The three-dimensional villiform-like carbon nanotube/cobalt silicate (CNT@Co silicate) core–shell nanocomposites were synthesi...περισσότερα »
Guanine nanowire based amplification strategy: Enzyme-free biosensing of nucleic acids and proteins
Alexandros G. SfakianakisστοΝοσολογίαΠριν από 1 ώρα
Publication date: 15 April 2016 *Source:*Biosensors and Bioelectronics, Volume 78 Author(s): Zhong Feng Gao, Yan Li Huang, Wang Ren, Hong Qun Luo, Nian Bing Li Sensitive and specific detection of nucleic acids and proteins plays a vital role in food, forensic screening, clinical and environmental monitoring. There remains a great challenge in the development of signal amplification method for biomolecules detection. Herein, we describe a novel signal amplification strategy based on the formation of guanine nanowire for quantitative detection of nucleic acids and proteins (thrombin)... περισσότερα »
A Prospective Study of Mortality and Trauma-Related Risk Factors Among a Nationally Representative Sample of Vietnam Veterans
Alexandros G. SfakianakisστοΟγκολογίαΠριν από 1 ώρα
Because Vietnam veterans comprise the majority of all living veterans and most are now older adults, the urgency and potential value of studying the long-term health effects of service in the Vietnam War, including effects on mortality, is increasing. The present study is the first prospective mortality assessment of a representative sample of Vietnam veterans. We used one of the longest follow-up periods to date (spanning older adulthood) and conducted one of the most comprehensive assessments of potential risk factors. Vital status and cause of death were ascertained for the 1,6...περισσότερα »
Targeting Mismatch Repair defects: a novel strategy for personalized cancer treatment
Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 1 ώρα
Publication date: Available online 2 December 2015 *Source:*DNA Repair Author(s): Rumena Begum, Sarah A. Martin The DNA Mismatch Repair (MMR) pathway is a fundamental cellular process required to repair mispaired bases introduced routinely during DNA replication. Given this critical role in the maintenance of genome stability, it is not surprising that underlying defects in the MMR pathway occur in both hereditary and sporadic cancers. Furthermore, the MMR status greatly influences the sensitivity of cells to many common chemotherapeutic agents. Therefore, novel strategies are being...περισσότερα »
Non-canonical actions of mismatch repair
Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 1 ώρα
Publication date: Available online 2 December 2015 *Source:*DNA Repair Author(s): Gray F. Crouse At the heart of the mismatch repair (MMR) system are proteins that recognize mismatches in DNA. Such mismatches can be mispairs involving normal or damaged bases or insertion/deletion loops due to strand misalignment. When such mispairs are generated during replication or recombination, MMR will direct removal of an incorrectly paired base or block recombination between nonidentical sequences. However, when mispairs are recognized outside the context of replication, proper strand discri... περισσότερα »
Endonuclease activities of MutLα and its homologs in DNA mismatch repair
Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 1 ώρα
Publication date: Available online 2 December 2015 *Source:*DNA Repair Author(s): Lyudmila Y. Kadyrova, Farid A. Kadyrov MutLα is a key component of the DNA mismatch repair system in eukaryotes. The DNA mismatch repair system has several genetic stabilization functions. Of these functions, DNA mismatch repair is a major one. The loss of MutLα abolishes DNA mismatch repair, thereby predisposing humans to cancer. MutLα has an endonuclease activity that is required for DNA mismatch repair. The endonuclease activity of MutLα depends on the DQHA(X)2E(X)4E motif which is a part of the act... περισσότερα »
7,12-Dimethylbenz(a)Anthracene-Induced Genotoxicity on Bone Marrow Cells from Mice Phenotypically Selected for Low Acute Inflammatory Response
Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 1 ώρα
Publication date: Available online 2 December 2015 *Source:*DNA Repair Author(s): Iana Suly Santos Katz, Layra Lucy Albuquerque, Alessandra Paes Suppa, Graziela Batista da Silva, José Ricardo Jensen, Andrea Borrego, Solange Massa, Nancy Starobinas, Wafa Hanna Koury Cabrera, Marcelo De Franco, Primavera Borelli, Olga Martinez Ibañez, Orlando Garcia Ribeiro Exposure to Polycyclic aromatic hydrocarbon (PAH) environmental contaminants has been associated with the development of mutations and cancer. 7,12-Dimethylbenz(a)Anthracene ( DMBA), a genotoxic agent, reacts with DNA directly, ind... περισσότερα »
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