Cyclic-stretch induces apoptosis in human periodontal ligament cells by activation of caspase-5

Publication date: January 2017
Source:Archives of Oral Biology, Volume 73
Author(s): Dan Zhao, Yaqin Wu, Chun Xu, Fuqiang Zhang
Background and objectiveAs caspase-5 plays a role in apoptosis, the present study aimed to identify the expression and activation of caspase-5 in human periodontal ligament cells in response to cyclic stretch and the role of caspase-5 in stretch-induced apoptosis.MethodsHuman PDL cells were exposed to 10% or 20% stretch strain for 6 or 24h, and the mRNA and protein expressions of caspase-5 were analyzed with real-time PCR and Western blot, respectively. The caspase-5 activation was detected by colorimetric assay. Then the influence of the inhibition of caspase-5 on the stretch-induced apoptosis and caspase-3 activation were analyzed with flow cytometry and colorimetric assay, respectively.ResultsBoth 6 and 24h stretches increased mRNA, protein expression and activation of caspase-5 in human PDL cells. Inhibition of caspase-5 inhibited the stretch-induced apoptosis and caspase-3 activation in human PDL cells.ConclusionThis study for the first time identified the expression and activation of caspase-5 in cyclic stretched human PDL cells and found that the stretch-induced apoptosis and caspase-3 activation were caspase-5 dependent.



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