Investigation of CD40 gene rs4810485 and rs1883832 mutations in patients with recurrent aphthous stomatitis

Publication date: February 2017
Source:Archives of Oral Biology, Volume 74
Author(s): Serbulent Yigit, Akin Tekcan, Aydin Rustemoglu, Mehmet Kemal Tumer, Goknur Kalkan, Kaan Yerliyurt
ObjectivesRecurrent aphthous stomatitis (RAS) is a common painful disorder affecting oral health, mucosa and overall quality of life. The etiopathogenesis of RAS remains unclear. RAS shows a large genetic diversity among the patients. In present study, we investigated whether CD40 gene rs4810485 and rs1883832 are associated with RAS and its clinical findings in Turkish patients.Materials and methodsGenomic DNA obtained from 387 individuals (160 patients with RAS and 227 healthy controls) were used in the study. CD40 gene rs4810485 and rs1883832 mutations were determined by using polymerase chain reaction with the specific primers.ResultsThere was no statistically significant difference between the groups with respect to genotype and allele distribution (p>0.05, OR 0.94, 95% CI 0.70–1.28, OR 1.01 95% CI 0.75–1.37, respectively). Additionally, there was no statistically significant difference in the combined genotype analysis of CD40 gene rs4810485 and rs1883832 mutations (p>0.05).ConclusionsAccording to our results, we found that CD40 gene mutations are not associated with RAS. We are convinced that CD40 gene mutations do not predispose to develop RAS in Turkish population. To our knowledge, this is the first study regarding CD40 gene rs4810485 and rs1883832 mutations investigated in RAS patients.



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