Determinants for selective transport of exogenously expressed cargo proteins into regulated and constitutive secretory pathways

Publication date: Available online 24 January 2017
Source:Journal of Oral Biosciences
Author(s): Junko Fujita-Yoshigaki, Meguami Yokoyama, Osamu Katsumata-Kato
BackgroundNewly synthesized secretory proteins are transported in vesicles and are sorted towards their appropriate destinations at trans-Golgi networks. It is thought that secretory proteins have sorting signals in their amino acid sequences that determine their destination. Therefore, many researchers have made efforts to identify these signals, using expression of exogenous cargo secretory proteins in various cell types. In this review, we will discuss these results in light of what will be necessary for future analysis of the sorting mechanisms.HighlightEndocrine and exocrine cells have two secretory pathways: regulated and constitutive. Sorting exogenously expressed proteins into these two pathways is dependent not only on the proteins themselves but also on the cell type. The results from in vivo experiments were more complex, where the same protein was delivered to different pathways in different cells.ConclusionIn order to study the sorting mechanism for secretory proteins, some evaluation of the cells' ability to synthesize, transport, and store the proteins is required. The HaloTag method has proved promising for the quantification of transportation in the two pathways.



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