Direct monitoring of basophil degranulation by using avidin-based probes

Publication date: Available online 20 April 2017
Source:Journal of Allergy and Clinical Immunology
Author(s): Régis Joulia, Claire Mailhol, Salvatore Valitutti, Alain Didier, Eric Espinosa

Teaser

We show that fluorescent avidin binds to basophil cell surface upon degranulation and can be used to set up a new basophil activation test (BAT). This new assay provides results comparable to those provided by the CD63 exposure-based BAT, but has the advantage to directly monitor granule exteriorization.


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