The effects of intermittent low-dose PTH treatment on rapid mandibular osteodistraction in rabbits

Publication date: Available online 19 April 2017
Source:Journal of Oral and Maxillofacial Surgery
Author(s): Bin Ye, Yunfeng Li, Songsong Zhu, Shengjun Sun, Jing Hu, Shujuan Zou
PurposeTo determine whether low-dose recombinant human parathyroid hormone (rhPTH) would have a beneficial effect on regenerate healing and surrounding bone in a rabbit rapid mandibular osteodistraction model.Materials and MethodsTwenty-eight rabbits underwent unilateral mandiblular lengthening at a rapid rate of 2mm/day for 5 days. From the first day of distraction, the experimental group received daily subcutaneous injections of 10μg/kg of rhPTH for 30 days and the control group received the same volume of saline( n=14 in each group). At 6 weeks after completion of distraction, the distracted callus was examined by micro-computed tomography (micro-CT), histology and three-point bending test. Moreover, the bone mineral density of anterior pin region was evaluated by dual energy X-ray absorptiometry (DXA).ResultsUnder a rapid distraction, poor bone healing was observed in the distracted callus from control group. However, more mature and abundant bone formation was found in the distracted callus from experimental group by histological and micro-CT examinations. Quantitatively, the PTH-treated animals had the greater parameters in bone volume fraction, trabecular number and trabecular thickness as well as mechanical properties when compared with controls (p < 0.05). Bone mineral density of anterior pin region was higher in the experimental than that in the control group (p < 0.05).ConclusionNot only can low-dose intermittent rhPTH administration enhance new bone formation but also it can prevent fixitor-related osteoporosis of surrounding segments following rapid mandibular osteodistraction in rabbits.



http://ift.tt/2pE4ui3