Morphometric characteristics of neutrophils stimulated by adhesion and hypochlorite

Publication date: July 2017
Source:Molecular Immunology, Volume 87
Author(s): Tatsiana Kuznetsova, Tatsiana Kulahava, Ivan Zholnerevich, Nadezda Amaegberi, Galina Semenkova, Oleg Shadyro, Juergen Arnhold
The aim of this work was to compare cell form, size and volume as well as the locomotor activity of polymorphonuclear leukocytes (PMNLs) stimulated by adhesion to glass and exposed to hypochlorous acid at non-toxic dose. After 20min of adhesion to a glass surface, volume, cell surface area and projection area of PMNLs were equaled to 143.1±21.4μm³, 288.8±28.8μm² and 248.3±32.3μm², respectively. Projection area of PMNLs exposed to NaOCl was noticeably enlarged as compared with control samples. The cell volume of 20min adherent cells exposed to NaOCl was enlarged in comparison with both control cells and 5min adhered exposed to NaOCl cells. NaOCl exposure induced a degranulation of PMNLs as measured by lysozyme release. Granules could be found both above the cell surface and on the substratum near the cell. The S/V ratio for PMNLs increased (from 1.52 to 2.02μm⁻¹) with the increasing of cell activation time. But at NaOCl addition the reverse tendency was observed (from 2.10 to 1.87μm⁻¹). In cells exposed to NaOCl the redistribution and decrease of concentration of F-actin took place. This observation supports the hypothesis that the priming of PMNLs with hypochlorous acid modifies cell motility and morphology and reflects also on other functions.



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