Effect of Choukroun’s platelet-rich fibrin combined with autologous micromorselized bone on the repair of mandibular defects in rabbits

Publication date: Available online 2 June 2017
Source:Journal of Oral and Maxillofacial Surgery
Author(s): Tian Zhou, Hua-Wei Yang, Zhuo-Wei Tian, Yang Wang, Xiao-Shan Tang, Jing-Zhou Hu
ObjectiveThe aim of this study was to explore the effect of Choukroun's platelet-rich fibrin (PRF) combined with autologous micromorselized bone on the repair of mandibular defects in rabbits.MethodsA total of 36 healthy New Zealand rabbits were selected in the present study. After the models of mandibular defects were established, rabbits were randomly divided into Choukroun's PRF, autologous micromorselized bone (autologous group), Choukroun's PRF combined with autologous bone (combined group) and model groups. After the rabbits were sacrificed at 2, 8 and 12 weeks postoperatively, their bone formation was assessed by X-ray and scanning electron microscopy (SEM), and the histological changes of the mandibular defect area were detected by hematoxylin and eosin (HE) staining. Cone-beam CT (CBCT) imaging was used to observe the size change of the mandibular defect area. The bone mineral density (BMD) was analyzed by the dual-energy X-ray absorptiometry.ResultsThe bone defect in the combined group showed better repair, increased bone mineral content (BMC) and denser callus than the other groups, and the defect area was filled with mature trabecular bone. In the Choukroun's PRF and autologous groups, the defect area was reduced and filled with osteoporotic trabecular bone. Clear mandibular defect area was still observed in the model group. Compared with the other groups, the combined group showed more bone regeneration, and more fibrous tissue regeneration, and higher bone maturity at all time points. The combined group had the highest BMD among the four groups, while there was no significant difference in the BMD between the Choukroun's PRF and autologous groups, and the BMD in the model group was the lowest. The BMD in four groups all increased with time. Conclusion: These findings indicate that Choukroun's PRF combined with autologous micromorselized bone can significantly improve the repair of mandibular defects in rabbits, and the effect is superior to Choukroun's PRF or autologous micromorselized bone alone.



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