Structural characterization of endogenous peroxidase activity in human, rat, hamster, and Suncus murinus salivary glands

Publication date: Available online 8 July 2017
Source:Journal of Oral Biosciences
Author(s): Keiichi Moriguchi, Michiya Utsumi, Takamichi Jogahara, Senichi Oda, Masaki Honda
Background3,3'-diaminobenzidine tetrazolium (DAB)-based methods have been developed to detect the localization of peroxidase (PO) activity. Histochemically, endogenous PO activity has been localized to acini of human and rodent salivary glands. This suggests that the enzyme may be used as a convenient marker of differentiated acinar cells to study factors that influence the development of the rat submandibular gland. We also investigated endogenous PO localization differences between rodent and insectivora (Suncus murinus) major salivary glands by light and electron microscopy. Moreover, ectopic sublingual gland-like tissue was found in the resin-embedded semi-thin sections of DAB-reacted hamster submandibular gland by confocal laser scanning microscopy.HighlightReflectance-mode confocal laser scanning microscopy was employed to visualize sites of PO activity in ectopic sublingual gland-like tissue of the hamster submandibular gland.ConclusionThe technique proposed here may result in novel applications for DAB-based histochemistry.



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