Source:Journal of Oral and Maxillofacial Surgery
Author(s): Danilo Siqueira Pino, Renato Correa Casarin, Suzana Peres Pimentel, Fabiano Ribeiro Cirano, Mônica Grazieli Corrêa, Fernanda Vieira Ribeiro
PurposeThis study aimed to determine the influence of resveratrol (RESV) on the repair of bone critical defects in calvaria of animals with induced diabetes mellitus (DM).Material and methodsOne hundred rats were divided into five groups: induced DM + RESV administration (DM+RESV; n = 20); induced DM + placebo solution administration (DM+PLAC; n = 20); induced DM + insulin therapy (DM+INS; n = 20); induced DM + administration of resveratrol and insulin (DM+RESV+INS; n = 20); and non-diabetic controls (NDM; n = 20). Diabetes was induced by intraperitoneal injection of 50 mg/kg streptozotocin, three days before surgical procedures. Two critical calvarial defects were created in each animal at the start of the study (day zero). Treatments were administered from day zero to day 30 of the experiment, when animals were euthanized. One of the defects was processed for histometric analysis to measure the closure of the bone defect. The tissue of the other defect was analyzed for quantification of BMP-2, OPN, OPG, RANKL, Runx2, Osx, β-catenin, Lrp-5, and Dkk-1 mRNA by quantitative PCR.ResultsHistometric results showed that the DM+RESV, DM+RESV+INS, and NDM groups exhibited greater closure of the bone defects as compared to the placebo- or insulin-treated groups (p < 0.05). Diabetic animals treated with RESV+INS showed higher levels of BMP-2 and Osx; Osx was also positively increased in animals treated with INS alone (p < 0.05).ConclusionsThe use of RESV, regardless of the presence of INS, positively influenced bone repair in DM-induced animals. Further, the combination of insulin with RESV is necessary for the modulation of BMP-2 gene expression.
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