Susceptibility of partially-desalivated rats to erosive tooth wear by calcium-supplemented beverages

Abstract

Objectives

To investigate the susceptibility of partially-desalivated rats to erosive tooth wear (ETW); the anti-erosive effect of a calcium-supplemented beverage; and the quantification of ETW by micro-computed tomography (micro-CT).

Methods

The study population consisted of thirty-eight rats, divided into partially-desalivated (n=19) and normal salivary flow (n=19). They were randomly allocated into 3 subgroups (n=6-7): A-diet soda, B-diet soda+calcium, C-water (control). Solutions were provided ad-libitum for 28 days, and the rats were euthanized afterwards. Each left hemi-mandible was scanned using micro-CT for enamel volume (3 molars) calculation. Visual analysis of photographs of the lingual surface of 1^st molars was performed independently by 3 blinded examiners. Data were statistically analysed (α=0.05).

Results

Micro-CT revealed no significant differences between partially-desalivated or normal groups. Rats consuming A had more enamel loss than those consuming B or C, which did not differ from each other. For visual analysis, desalivation did not affect ETW. Rats consuming C showed the lowest ETW, followed by B and then A, for both partially-desalivated and normal rats. Spearman correlation between the two ETW quantification methods was -0.65.

Conclusions

Partial desalivation did not increase ETW. Ca-containing beverage prevented ETW. Micro-CT quantified ETW, although it was not as sensitive as visual analysis.

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