P14 methylation - an epigenetic signature of salivary gland mucoepidermoid carcinoma in serbian population

Publication date: Available online 28 September 2017
Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Author(s): Nadja Nikolic, Jelena Carkic, Ivana Ilic Dimitrijevic, Najib Eljabo, Milena Radunovic, Boban Anicic, Nasta Tanic, Markus Falk, Jelena Milasin
ObjectivesTo investigate the prevalence of p16^INK4a, p14^ARF, TP53 and hTERT promoter hypermethylation in mucoepiermoid carcinomas (MECs), and search for a possible association between methylation status and clinicopathological parameters.Study designDNA extracted from 35 formalin fixed and paraffin embedded MEC samples and 10 normal salivary glands (NSGs) tissue was analyzed for the presence of promoter hypermethylation using methylation specific PCR.ResultsThe percentages of gene hypermethylation in MECs versus NSGs were the following- p14:100% vs. 20% (P<0.001), p16: 60% vs. 20% (P=0.035), hTERT: 54.3% vs. 20% (P=0.078) and TP53: 31.4% vs. 30% (P=0.981). Multiple sites were found to be methylated in 86% of MECs compared to 10% in NSGs (P<0.001). TP53 and hTERT were more frequently methylated in lower clinical stages (P=0.033 and P=0.005, respectively).ConclusionsHypermethylation of p14 appears to be an important event in the development of mucoepidermoid carcinoma. High frequency of gene hypermethylation and high incidence of methylation at multiple sites point to the importance of epigenetic phenomena in the pathogenesis of MECs, although with modest impact on clinical parameters.



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