Peripheral blood monocytes reveal an activated phenotype in pediatric uveitis

Publication date: Available online 17 September 2017
Source:Clinical Immunology
Author(s): Karoline Walscheid, Lisa Neekamp, Arnd Heiligenhaus, Toni Weinhage, Dirk Holzinger, Carsten Heinz, Maren Kasper, Dirk Foell
ObjectiveTo characterize peripheral blood monocytes in uveitis associated with juvenile idiopathic arthritis (JIAU).MethodsPeripheral blood monocytes from children with JIA (either with (n=18) or without uveitis (n=11)), idiopathic anterior uveitis (IAU; n=12) and healthy controls (n=11) were analyzed by flow cytometry.ResultsPercentage of CD14+CD86+ monocytes and CD86 expression on single cell level were significantly higher in all patient groups than in controls, whereas no major differences existed between patient groups. Frequency of CD39+ (p<0.05 all groups) and CD73+ monocytes (p=0.03 JIAU vs controls) was elevated in patients. Disease activity did not influence monocyte phenotypes, but in methotrexate-treated patients numbers of CCR2+ monocytes were reduced and numbers of CD86+ and CD39+ cells increased.ConclusionMonocytes from children with JIAU display a distinct phenotype when compared to cells from healthy children. Phenotypic changes seem to be neither arthritis- nor uveitis-dependent, but may be modified by treatment.



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