Αρχειοθήκη ιστολογίου

Αλέξανδρος Γ. Σφακιανάκης
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5
Άγιος Νικόλαος Κρήτη 72100
2841026182
6032607174

Σάββατο 30 Δεκεμβρίου 2017

Sex-based differences in CD103+ dendritic cells promote female-predominant Th2 cytokine production during allergic asthma

Abstract

Background

Gender disparities in adult patients with asthma regarding its prevalence and severity are mainly due to enhanced type 2 T-helper (Th2) cytokine production in female patients compared to that in male patients. However, the pathways mediating this effect remain unclear.

Objective

We aimed to determine the roles of two major subsets of dendritic cells (DCs) in females, specifically those displaying CD11b or CD103, during enhanced Th2-priming after allergen exposure, using an ovalbumin-induced asthma mouse model.

Methods

Sex-based differences in the number of DCs at inflamed sites, co-stimulatory molecule expression on DCs, and the ability of DCs to differentiate naïve CD4+ T cells into Th2 population were evaluated after allergen exposure in asthmatic mice. In addition, we assessed the role of 17β-estradiol in CD103+ DC function during Th2-priming in vitro.

Results

The number of CD11bhigh DCs and CD103+ DCs in the lung and bronchial lymph node (BLN) were increased to a greater extent in female mice than in male mice at 16 to 20 hours after ovalbumin (OVA) inhalation. In BLNs, CD86 and I-A/I-E expression levels and antigen uptake ability in CD103+ DCs, but not in CD11bhigh DCs, were greater in female mice than in male mice. Furthermore, CD4+ T cells cultured with CD103+ DCs from female mice produced higher levels of interleukin (IL)-4, IL-5, and IL-13, compared with CD4+ T cells cultured with CD103+ DCs from male mice. The 17β-estradiol oriented-enhancement of CD86 expression on CD103+ DCs after allergen exposure induced the enhanced IL-5 production from CD4+ T cells.

Conclusions & Clinical Relevance

These findings suggest that with regards to asthma, enhanced Th2 cytokine production in females might be attributed to 17β-estradiol-mediated Th2-oriented CD103+ DCs in the BLN.

This article is protected by copyright. All rights reserved.



http://ift.tt/2CqaUrG

Δεν υπάρχουν σχόλια:

Δημοσίευση σχολίου