Herpesviruses viral loads and levels of proinflammatory cytokines in apical periodontitis

Abstract

Objectives

This study aimed to analyse Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) viral loads in symptomatic and asymptomatic apical periodontitis lesions, to determine levels of TNF-α, IL-1β, and IL-6 in these lesions and to investigate a possible correlation between herpesviral copy numbers and levels of proinflammatory cytokines .

Materials and methods

100 samples of apical periodontitis were subjected to HCMV and EBV copy numbers analysis by nested polymerase chain reaction (PCR) and TaqMan real-time PCR. The concentrations of TNF-α, IL-1β and IL-6 were determined by ELISA method. SPSS software was used for statistical analysis.

Results

There were no significant differences in the occurrence of EBV and HCMV between symptomatic and asymptomatic periapical lesions (P=.686, P=.879, respectively). Only 12 out of 74 EBV (16.2%) and 4 out of 54 HCMV (13.5%) nested PCR positive samples showed increased viral copy numbers above the limit of 125 copies/ml. There was no significant correlation between the levels of analysed proinflammatory cytokines and herpesviral copy numbers in our sample.

Conclusion

The observed low viral loads point to a relatively rare occurrence of active EBV and HCMV infection in our sample. Latent herpesviral infection does not enhance the production of investigated proinflammatory cytokines.

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