Αρχειοθήκη ιστολογίου

Αλέξανδρος Γ. Σφακιανάκης
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5
Άγιος Νικόλαος Κρήτη 72100
2841026182
6032607174

Σάββατο 17 Μαρτίου 2018

Mycoplasma pneumoniae induces allergy by producing p1-specific IgE

Publication date: Available online 16 March 2018
Source:Annals of Allergy, Asthma & Immunology
Author(s): Qing Ye, Jian-hua Mao, Qiang Shu, Shi-qiang Shang
BackgroundOur previous study found that most of MPP patients had elevated serum total IgE levels.ObjectiveThe purpose of this paper is to determine components of mycoplasma pneumoniae that can cause IgE increase in children, and to clarify its specific mechanism.MethodsThe components of MP cells were isolated by serum IgE from patients with MP pneumonia (MPP). These components obtained through the prokaryotic expression were used as allergens to detect the proportion of allergen-specific IgE produced in MPP patients, and the clinical characteristics and related immune parameters of these patients who produced this allergen-specific IgE were also analyzed. In addition, cell experiment was used to verify the biological effect of these components in vitro.ResultsP1-specific IgE was detected in serum of MPP children. An about 24-kDa polypeptide of P1 protein was obtained through prokaryotic expression purified by nickel agarose affinity chromatography. Approximately 9.2% of MPP patients produced IgE against this polypeptide of P1 protein, which was more likely to be produced in MPP patients with no history of allergies or family history of allergy-related diseases. P1-specific IgE-positive MPP patients had more severe clinical symptoms, with excessive secretion of IL-4 and IL-5 and over-differentiation of Th0 cells into Th2 cells. Tests also demonstrated that the P1 protein stimulated excessive secretion of IL-4 and IL-5 in PBMCs from the peripheral blood of healthy donors.ConclusionMP is not only an infectious agent but also an allergen for certain individuals. The P1 protein of MP can induce the production of P1-specific IgE.



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