Influence of Platelet-Poor Plasma on angiogenesis and maintenance of volume in autogenous bone grafts

Publication date: Available online 27 June 2018
Source:Journal of Oral and Maxillofacial Surgery
Author(s): Jonas Dantas Batista, Pedro Henrique Justino Oliveira Limirio, Flaviana Soares Rocha, Camilla Christian Gomes Moura, Darceny Zanetta-Barbosa, Paula Dechichi
PurposeThe aim of this study was to evaluate the effect of different storage media on angiogenesis and maintaining autogenous bone graft volume in rabbits.Material and MethodsTwo grafts were removed bilaterally from calvaria of 18 rabbits. One graft was removed and immediately fixed in the right mandibular angle (Control group). The other graft was stored for 30 minutes in one of the following storage media (n=6): Saline solution (Saline group), Air exposure (Dry group), Platelet-poor plasma (PPP group), and then screw-retained in the right mandibular angle, in the same animal. Four weeks later the animals were euthanized, and the grafted areas were harvested and fixed in 10% phosphate buffered formaldehyde solution and embedded in paraffin. The 5μm semi-sections were stained in hematoxylin-eosin and mallory trichrome.ResultsThe histological analysis of all groups showed bone graft vascularized and well-incorporated into the recipient site. Blood vessels decreased in number in the Saline and Dry groups compared with Control groups (p<0.03); however, blood vessels increased in the PPP group (p<0.05). Osteoclasts were lower in number than in Saline group compared with Control group (p<0.05). Furthermore, the Saline group showed higher numbers of blood vessels than Dry group (p<0.01). PPP group showed higher bone graft volume values compared with Dry and Saline Group (p<0.01). In addition, Saline group showed higher bone graft volume values than Dry group (p<0.01).ConclusionsThe PPP improved angiogenesis, maintained volume of autogenous bone graft, and was a better storage media in the trans-surgical period than dry and saline solution.



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