Αρχειοθήκη ιστολογίου

Αλέξανδρος Γ. Σφακιανάκης
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5
Άγιος Νικόλαος Κρήτη 72100
2841026182
6032607174

Δευτέρα 9 Ιουλίου 2018

Epithelial IL-15 Is a Critical Regulator of {gamma}{delta} Intraepithelial Lymphocyte Motility within the Intestinal Mucosa [MUCOSAL IMMUNOLOGY]

Intraepithelial lymphocytes (IELs) expressing the TCR ( IELs) provide continuous surveillance of the intestinal epithelium. However, the mechanisms regulating the basal motility of these cells within the epithelial compartment have not been well defined. We investigated whether IL-15 contributes to IEL localization and migratory behavior in addition to its role in IEL differentiation and survival. Using advanced live cell imaging techniques in mice, we find that compartmentalized overexpression of IL-15 in the lamina propria shifts the distribution of T cells from the epithelial compartment to the lamina propria. This mislocalization could be rescued by epithelial IL-15 overexpression, indicating that epithelial IL-15 is essential for IEL migration into the epithelium. Furthermore, in vitro analyses demonstrated that exogenous IL-15 stimulates IEL migration into cultured epithelial monolayers, and inhibition of IL-2Rβ significantly attenuates the basal motility of these cells. Intravital microscopy showed that impaired IL-2Rβ signaling induced IEL idling within the lateral intercellular space, which resulted in increased early pathogen invasion. Similarly, the redistribution of T cells to the lamina propria due to local IL-15 overproduction also enhanced bacterial translocation. These findings thus reveal a novel role for IL-15 in mediating T cell localization within the intestinal mucosa and regulating IEL motility and patrolling behavior as a critical component of host defense.



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