Effect of calcium fluoride on the activity of dentin matrix-bound enzymes

Publication date: Available online 11 September 2018

Source: Archives of Oral Biology

Author(s): Pinar Altinci, Murat Mutluay, Leo Tjäderhane, Arzu Tezvergil-Mutluay

Abstract

Objective

Matrix metalloproteinases (MMPs) and cysteine cathepsins (CCs) are two distinct enzymatic pathways responsible for the degradation of collagen fibrils in demineralized dentin. NaF and KF have been shown to inhibit salivary MMP-2, -9 and CCs. This study investigated the inhibitory effect of calcium fluoride (CaF₂) on the dentin matrix-bound MMPs and CCs.

Design

Phosphoric acid (10%)-demineralized dentin beams (1 × 2×6 mm) were incubated at 37 °C in an 1 mL of artificial saliva (AS, control), or AS with 6, 12, 24, 48, 120. 179 and 238 mM F containing CaF₂ (n = 10/group) for 1, 7 and 21 days. All groups were further incubated in AS only for 6 months. Total MMP activity, dry mass loss, CTX and hydroxyproline (HYP) analyses were performed after each incubation. The beams were examined under scanning electron microscopy (SEM). MMP-2 and MMP-9 activities were screened with gelatin zymography.Data were analyzed by using ANOVA and Tukey HSD tests (p = .05).

Results

The total MMP activity was similar for all groups after 21 days and 6 months. After 21 days, the cumulative mass loss and CTX levels were lower compared to control for the CaF₂ ≥48 and CaF₂≥120 mM, respectively (p < .05). After 6 months, no significant difference was detected in the dry mass loss and CTX compared to the control (p > .05), whereas HYP level was higher with F 24 and 238 mM groups. CaF₂-like minerals were observed on the beams under SEM. There was no gelatinase inhibition in zymography.

Conclusion

CaF₂ does not prevent the degradation of demineralized dentin matrices due to the catalytic activity of MMPs and CCs.

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