Αρχειοθήκη ιστολογίου

Αλέξανδρος Γ. Σφακιανάκης
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5
Άγιος Νικόλαος Κρήτη 72100
2841026182
6032607174

Τετάρτη 17 Οκτωβρίου 2018

The effect of compressive force combined with mechanical vibration on human alveolar bone osteoblasts

Publication date: Available online 16 October 2018

Source: Journal of Oral Biology and Craniofacial Research

Author(s): Chatchai Chatmahamongkol, Anute Pravitharangul, Srisurang Suttapreyasri, Chidchanok Leethanakul

Abstract
Objective

This study aimed to investigate the effects of compressive force combined with mechanical vibration on the expression of pro-inflammatory cytokines that promote osteoclastogenesis and related to orthodontic tooth movement acceleration in human alveolar bone osteoblasts in vitro.

Methods

Osteoblasts were subjected to compressive force (C), mechanical vibration (V), compressive force combined with mechanical vibration (CV), or no force as a control for 12, 24 and 48 h. Interleukin-1 beta (IL-1β), interleukin-6 (IL-6), receptor activator of nuclear factor kappa-Β ligand (RANKL) and osteoprotegerin (OPG) mRNA and protein expression were assessed using quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assays.

Results

In C and CV groups, IL-1β and IL-6 mRNA and protein expression were significantly higher and OPG mRNA and protein expression were significantly lower than control and V groups. However, the expressions were not different between C and CV groups. RANKL mRNA and protein expression were not different between any groups. While, OPG mRNA and protein expression in V group were significantly higher than control group.

Conclusions

Vibration neither enhanced nor inhibited the expression of IL-1β, IL-6, RANKL and OPG in compressed human alveolar bone osteoblasts.



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