An unexpected protective role of low-affinity allergen-specific IgG through the inhibitory receptor FcγRIIb

Publication date: November 2018

Source: Journal of Allergy and Clinical Immunology, Volume 142, Issue 5

Author(s): Lisha Zha, Fabiana M.S. Leoratti, Lichun He, Mona O. Mohsen, Mark Cragg, Federico Storni, Monique Vogel, Martin F. Bachmann

Background

Induction of allergen-specific IgG antibodies is a critical parameter for successful allergen-specific immunotherapy. IgG antibodies can inhibit IgE-mediated mast cell activation through direct allergen neutralization or through the inhibitory receptor FcγRIIb. The affinity of IgE antibodies to the allergen has been shown to be critical for cellular activation.

Objective

Here we addressed the question of affinity thresholds of allergen-specific IgG antibodies for inhibition of mast cell activation using 2 different mAbs against the major cat allergen Fel d 1 both in vitro and in vivo in mice.

Methods

Sequences of the 2 high-affinity mAbs were back-mutated to germline, resulting in low-affinity (10⁻⁷ mol/L) antibodies of the exact same specificity.

Results

Using these newly generated recombinant antibodies, we demonstrate that low-affinity antibodies are still able to inhibit mast cell activation through FcγRIIb but do not neutralize the allergen.

Conclusion

Antibody affinity dictates the mechanism of mast cell inhibition, and IgG antibodies triggering the inhibitory FcγRIIb pathway can show a broader cross-reactivity pattern than previously thought. This indicates that allergen-specific immunotherapy generates a larger protective umbrella of inhibitory IgG antibodies than previously appreciated.

Graphical abstract

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