Bone regeneration of canine peri-implant defects using cell sheets of adipose-derived mesenchymal stem cells and platelet-rich fibrin membranes

Publication date: Available online 1 November 2018

Source: Journal of Oral and Maxillofacial Surgery

Author(s): Lidan Ding, Shijun Tang, Panpan Liang, Chao Wang, Peng fei Zhou, Leilei Zheng

Abstract

Purpose

Insufficient bone volume compromises the success rate and osseointegration of immediate implantation. The objective of the present study was to engineer bone tissue by using adipose-derived stem cell (ASC) sheets and autologous platelet-rich fibrin (PRF) to enhance new bone formation and osseointegration around dental implants.

Material and Methods

The proliferation and osteogenic potential of ASCs treated with autologous PRF were evaluated with CCK-8 assays, alkaline phosphatase (ALP) staining, and real-time quantitative PCR. A three-wall bone defect around each immediate implant was generated in the mandible and randomly treated with one of the following four groups: A, ASC sheets/PRF complex; B, ASC sheets only; C, PRF only; and D, no treatment. Micro-CT, biomechanical tests, fluorescent bone labeling and histological assessments were performed to evaluate bone regeneration capacity.

Results

The proliferation and osteogenic potential of canine ASCs were significantly enhanced by PRF. Group A exhibited significantly more new bone formation and a higher reosseointegration percentage (41.17+ 1.44%, 55.06+ 0.06%) than that of the other three groups. Fluorescent labeling revealed that the most rapid bone remodeling activity occurred in group A. (P<0.05).

Conclusion

These results suggest that sheets of ASC combined with autologous PRF may be a promising tissue-engineering strategy for bone formation in immediate implantation.

https://ift.tt/2SDeYwp