Effect of technetium‐99 conjugated with methylene diphosphonate (99Tc‐MDP) on OPG/RANKL/RANK system in vitro

Abstract

Background

RANKL and RANK play an important role in jaw resorption during the development of the ameloblastomas. Therefore, the aim of this study was to explore the effect of ⁹⁹Tc‑MDP on OPG/RANKL/RANK system on RAW264.7 and MC3T3‐E1 cell lines in vitro, and provide the theoretical basis for the clinical treatment of the jaw ameloblastoma.

Methods

Different concentrations of ⁹⁹Tc‐MDP were used to treat RAW264.7 and MC3T3‐E1 cell lines. The cell proliferative inhibition rate was analyzed by CCK‐8. Cell apoptosis and cell cycle were detected by flow cytometry. Western blot were used to detect the expression of OPG, RANKL and RANK.

Results

Treatment of RAW264.7 cell lines with different concentrations of ⁹⁹Tc‐MDP had inhibitory effects and decreased the expression of RANK protein. The cell proliferation of ⁹⁹Tc‐MDP on MC3T3‐E1cell lines was stronger at 48h than at 24h except for 100μg/ml concentration group. Compared with the concentration of 0.01μg/ml, the treatment of MC3T3‐E1 cells with 100μg/ml ⁹⁹Tc‐MDP showed that the cell proliferative effect decreased at 24h and 48h (P<0.05). After treatment with 0.01μg/ml ⁹⁹Tc‐MDP, the expression of OPG in MC3T3‐E1 cells was significantly increased (P<0.05). Compared with 0.01μg/ml, the expression of RANKL was decreased after treatment with 100μg/ml ⁹⁹Tc‐MDP (P<0.05).

Conclusion

⁹⁹Tc‐MDP can induce apoptosis of RAW264.7 cells and inhibit the expression of RANK protein. The effect of 0.01μg/ml of low concentration of ⁹⁹Tc‐MDP can promote the proliferation of MC3T3‐E1 cells and increase the expression of OPG and RANKL protein. ⁹⁹Tc‐MDP may have adjuvant therapeutic effects on the treatment of jaw ameloblastoma.

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