Αρχειοθήκη ιστολογίου

Αλέξανδρος Γ. Σφακιανάκης
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5
Άγιος Νικόλαος Κρήτη 72100
2841026182
6032607174

Τρίτη 22 Ιανουαρίου 2019

Azithromycin and Erythromycin susceptibility and macrolide resistance genes in Prevotella from patients with periodontal disease

Abstract

Objectives

To study oral Prevotella spp. isolated from patients with chronic periodontitis, to determine their susceptibility to azithromycin and erythromycin and to screen the presence of macrolide resistance genes therein.

Material and Methods

Isolates with a Prevotella‐like morphology were obtained from subgingival samples of 52 patients with chronic periodontitis. Each isolate was identified to the species level by sequencing of the 16S rRNA gene. In 100 Prevotella spp. isolates, azithromycin and erythromycin susceptibility was determined using the E‐test method, and the screening of erm(A), erm(B), erm(C), erm(F), erm(G), erm(Q) and mef(A) genes was done by PCR.

Results

Prevotella intermedia and Prevotella nigrescens were the most identified species (33% each). MIC ranges for both antibiotics were 0.016/0.032 ‐ >256 μg/mL. MIC50 values for azithromycin and erythromycin were 1.5 and 1 μg/mL, respectively, and MIC90 values were >256 μg/mL for both antibiotics. Nineteen percent of the isolates carried erm(B) and 51% carried erm(F).

Conclusions

The MIC values found were high compared to previous studies. erm(F) was greatly prevalent and we describe for the first time the erm(B) gene in Prevotella spp. The presence of either of the genes seems to be associated with a higher degree of resistance to azithromycin and erythromycin.

This article is protected by copyright. All rights reserved.



http://bit.ly/2RJL111

Δεν υπάρχουν σχόλια:

Δημοσίευση σχολίου