Optimal, Large-Scale Propagation of Mouse Mammary Tumor Organoids

alexandrossfakianakis shared this article with you from Inoreader Optimal, Large-Scale Propagation of Mouse Mammary Tumor Organoids Via Journal of Mammary Gland Biology and Neoplasia AbstractTumor organoids mimic the architecture and heterogeneity of in vivo tumors and enable studies of collective interactions between tumor cells as well as with their surrounding microenvironment. Although tumor organoids hold significant promise as cancer models, they are also more costly and labor-intensive to cultivate than traditional 2D cell culture. We sought to identify critical factors regulating organoid growth ex vivo, and to use these observations to develop a more efficient organoid expansion method. Using time-lapse imaging of mouse mammary tumor organoids in 3D culture, we observed that outgrowth potential varies non-linearly with initial organoid size. Maximal outgrowth occurred in organoids with a starting size between ~10 to 1000 cells. Based on these observations, we ... View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.