MicroRNA-449a inhibits cell proliferation and migration by regulating mutant p53 in MDA-MB-468 cells

xlomafota13 shared this article with you from Inoreader MicroRNA-449a inhibits cell proliferation and migration by regulating mutant p53 in MDA-MB-468 cells Via Experimental and therapeutic medicine Exp Ther Med. 2021 Sep;22(3):1020. doi: 10.3892/etm.2021.10452. Epub 2021 Jul 15. ABSTRACT The present study aimed to investigate the role of microRNA (miR)-449a in the proliferation, migration and apoptosis of MDA-MB-468 breast cancer cells and examine the association between miR-449a and mutant p53 in these cells. Cell proliferation, migration and invasion were examined using a crystal violet staining assay, wound healing scratch assay and Transwell assay, respectively. The expression level of miR-449a and p53 was detected by reverse transcription-quantitative PCR or western blotting. The results indicated that knockdown of mutant p53 suppressed the proliferation and migration of MDA-MB-468 cells by inhibiting the PI3K/AKT/mTOR signaling pathway. In addition, miR-449a suppressed proliferation and migration via downregulation of mutant p53 expression in MDA-MB-468 cells. Therefore, miR-449a may function as a tumor suppressor by regula ting p53 expression in breast cancer cells, which may have potential implications in the treatment of patients with triple-negative breast cancer carrying mutant p53. PMID:34373706 | PMC:PMC8343910 | DOI:10.3892/etm.2021.10452 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.