Understanding orthopedic infections through a different perspective: Microcalorimetry growth curves

xlomafota13 shared this article with you from Inoreader Understanding orthopedic infections through a different perspective: Microcalorimetry growth curves Via Experimental and therapeutic medicine Exp Ther Med. 2022 Apr;23(4):263. doi: 10.3892/etm.2022.11189. Epub 2022 Feb 4. ABSTRACT Infectious disease is a serious healthcare problem in orthopedics, as well as other surgical specialties. Accurate and prompt diagnosis, as well as proper care, is critical, as infection of a surgical wound, particularly in the case of arthroplasties or the use of orthopedic implants, can have a catastrophic effect in most cases, necessitating the removal of foreign material. Lyophilized bacteria samples were obtained from Cantacuzino National Institute of Research and Development for Microbiology and Immunology and investigated microcalorimetrically. Isothermal microcalorimetry measures the temperature generated by the multiplication of microorganisms; using an adapted program, it describes the growth curve in real-time according to the received electrical signal. The thermograms of Escherichia coli and Klebsiella pneumonie were anal yzed, and similarities were observed for both the time required for the bacteria to grow and the heat flow generated by their growth. Bacterial microcalorimetry has a variety of benefits, and should be regarded as a means of rapid and accurate diagnosis. Sensitivity is a valuable attribute for a diagnostic technique; when only a few microorganisms are present, microcalorimetric signs of bacterial multiplication can be observed. Microcalorimetry has potential as a simple diagnostic tool in a variety of infections, but further research is needed to ensure that it is used correctly. A thorough investigation (including kinetic analysis) of a reproducible thermal signal of bacterial growth could lead to the creation of new methods for quickly identifying bacteria. PMID:35251329 | PMC:PMC8892613 | DOI:10.3892/etm.2022.11189 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.