Αρχειοθήκη ιστολογίου

Αλέξανδρος Γ. Σφακιανάκης
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5
Άγιος Νικόλαος Κρήτη 72100
2841026182
6032607174

Τετάρτη 23 Νοεμβρίου 2022

Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats

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Abstract

Aim

The aim of this study was to evaluate the effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis (EP) in rats.

Methods

Thirty-two rats were divided into four groups: control, C-HD100 (B. bacteriovorus), EP, and EP-HD100. On day 0, EP was induced by placement of cotton ligatures around the mandibular first molars (MFMs) in the EP and EP-HD100 groups. In the C-HD100 and EP-HD100 groups, suspensions containing 1 × 109 PUF/mL of B. bacteriovorus HD100 were topically administered to the subgingival region of MFMs on days 0, 3, and 7. Animals were euthanized on day 14. Morphometrics analysis were performed in hemimandibles. The levels of Tumor necrosis factor alpha (TNF-α), Interleukin (IL)-6, Monocyte chemoattractant protein (MCP)-1, IL-10, IL-1β, Transforming growth factor beta (TGF-β), Macrophage colony-stimulating factor (M-CSF) and Regulated on activation and normal T cell expressed and secreted (RANTES) were determined by enzymatic immunoassays in gingival tissues. Beta Defensin (BD)-1, BD-2 and BD-3, Toll Like Receptors (TLR)-2 and TLR-4, and Cluster Differentiation ( CD)-4, CD-8 and CD-57 were analyzed by immunohistochemistry in hemimandibles. Data were statistically analyzed.

Results

The EP group showed greater alveolar bone loss than EP-HD100 (p < 0.05). The EP-HD100 group showed higher levels of MCP-1, RANTES, IL-10, and TGF-β, lower levels of TNF-α than the EP group (p < 0.05). No differences were observed in IL-1β, IL-6, and M-CSF levels between EP and EP-HD100 groups. The C-HD100 group had higher IL-6, TNF-α, RANTES and MCP-1 levels than the control group (p < 0.05). Regarding BD, the EP-HD100 group showed a larger immunolabeling pattern for BD-1, BD-2, and BD-3 than the EP group (p < 0.05). No significant differences in the immunolabeling pattern were observed for TLR-2, TLR-4, CD4, CD8, and CD57 between EP and EP-HD100 groups.

Conclusion

The topical use of B. bacteriovorus HD100 reduces alveolar bone loss, increases expression of beta defensin, and modulates the cytokines levels on periodontal tissues in rats with EP.

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