! ORL via Alexandros G.Sfakianakis on Inoreader: Expression of Surfactant Protein-A during LPS-Induced Otitis Media with Effusion in Mice

! ORL via Alexandros G.Sfakianakis on Inoreader

Expression of Surfactant Protein-A during LPS-Induced Otitis Media with Effusion in Mice Sep 2nd 2015, 00:32, by Li, L., Guo, X., Olszewski, E., Fan, Z., Ai, Y., Han, Y., Xu, L., Li, J., Wang, H. Objective The objective of this study was to investigate the expression and role of surfactant protein (SP) in the middle ear throughout lipopolysaccharide (LPS)-induced otitis media with effusion (OME). Study Design Randomized case-controlled animal model. Setting Shandong University, Jinan, China. Subjects and Methods SP expression was monitored using reverse transcription polymerase chain reaction (PCR) in normal mice (n = 5). Two groups, control phosphate-buffered saline–injected mice (n = 5) and LPS-injected mice (n = 5), were euthanized 5 days following injection. RNA was extracted for reverse transcription PCR and real-time PCR, and temporal bone samples were used for hematoxylin and eosin staining. LPS was injected into mice, and 5 mice per test were euthanized at 0, 12, 24, 48, 72, and 96 hours following injection. For mRNA expression quantification, reverse transcription PCR and real-time PCR were performed, and proinflammatory cytokine levels were measured by enzyme-linked immunosorbent assay. Results SP-A and SP-D expression was detected in normal murine Eustachian tubes. SP-A expression was up-regulated after LPS-induced OME (P = .01). At various time points after LPS injection, concentrations of proinflammatory cytokines (tumor necrosis factor–α [TNF-α], interleukin (IL)–1β, and IL-6) in the middle ear increased significantly (P < .05) and correlated with changes in SP-A expression. Conclusion SP-A and SP-D exist in the murine middle ear. The expression of SP-A and TNF-α, IL-1β, and IL-6 was up-regulated in the middle ear of the LPS-induced OME animal model.

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