Enhanced regeneration potential of mobilized dental pulp stem cells from immature teeth

Abstract

Objectives

We have previously demonstrated that dental pulp stem cell (DPSCs) isolated from mature teeth by granulocyte-colony stimulating factor (G-CSF)-induced mobilization method can enhance angiogenesis/vasculogenesis and improve pulp regeneration when compared with colony-derived DPSCs. However, the efficacy of this method in immature teeth with root-formative stage has never been investigated. Therefore, the aim of this study is to examine the stemness, biological characteristics and regeneration potential in mobilized-DPSCs compared with colony-derived DPSCs from immature teeth.

Materials And Methods

Mobilized-DPSCs isolated from immature teeth were compared to colony-derived DPSCs using methods including flow cytometry, migration assays, mRNA expression of angiogenic/neurotrophic factor, and induced differentiation assays. They were also compared in trophic effects of the secretome. Regeneration potential was further compared in an ectopic tooth transplantation model.

Results

Mobilized-DPSCs had higher migration ability and expressed more angiogenic/neurotrophic factors than DPSCs. The mobilized-DPSC secretome produced a higher stimulatory effect on migration, immunomodulation, anti-apoptosis, endothelial differentiation and neurite extension. In addition, vascularization and pulp regeneration potential were higher in mobilized-DPSCs than in DPSCs.

Conclusions

G-CSF-induced mobilization method enhances regeneration potential of colony-derived DPSCs from immature teeth.

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