Chromosome instability in tumor resection margins of primary OSCC is a predictor of local recurrence

Publication date: March 2017
Source:Oral Oncology, Volume 66
Author(s): Damiana D.C.G. Pierssens, Maarten C. Borgemeester, Stijn J.H. van der Heijden, Carine J. Peutz-Kootstra, Andrea M. Ruland, Annick M. Haesevoets, Peter A.W.H. Kessler, Bernd Kremer, Ernst-Jan M. Speel
BackgroundThe local recurrence rate in oral squamous cell cancer (OSCC) hardly decreases. This is partly due to the presence of (pre)malignant cells in the remaining tissue after resection, that may lead to the development of a new tumor in time. Detection of histologically (pre)malignant cells in the tumor resection margins should predict these patients at risk for recurrence, however this appears to be difficult in routine practice. Purpose of this study was to apply easy-to-use molecular tests for more accurate detection of (pre)malignant cells in histopathologically tumor-free margins, to improve diagnosis of patients at risk.Methods42 patients with firstly diagnosed, radically resected primary OSCC with histopathologically confirmed tumor-free resection margins (treated between 1994 and 2003) were included. Inclusion criteria comprised of follow-up ⩾5years, and radical surgery without postoperative treatment. Formalin-fixed paraffine-embedded tissue sections of 42 tumors, 290 resection margins, and 11 recurrences were subjected to fluorescence in situ hybridization (FISH) to examine chromosome 1 and 7 copy number variations (CNV), and to p53 immunohistochemistry (IHC).Results11 out of the 42 patients developed a local recurrence within 5years. FISH analysis showed that nine of eleven recurrences exhibited CI in at least one of the resection margins (p=0.008). P53 overexpression and routine histopathologic classification were not correlated with recurrent disease. The presence of CI in the resection margins revealed a significantly worse progression-free survival (log-rank p=0.012).ConclusionsCI in the resection margins of OSCC can reliably identify patients at risk for developing a local recurrence.



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