Effects of collagen membrane application and cortical bone perforation on de novo bone formation in periosteal distraction: an experimental study in a rabbit calvaria

Publication date: February 2017
Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, Volume 123, Issue 2
Author(s): Ken Nakahara, Maiko Haga-Tsujimura, Kosaku Sawada, Matthias Mottini, Benoit Schaller, Nikola Saulacic
ObjectivesThe aim of the present study was to assess the impact of collagen membrane application and cortical bone perforations in periosteal distraction osteogenesis.Study DesignA total of 32 New Zealand rabbits were randomized into four experimental groups, considering two treatment modalities. Calvarial bone was perforated or left intact (P+/−). In half the animals, the distraction mesh was covered with a collagen membrane (M+/−). All animals were subjected to a 7-day latency period and a 10-day distraction period. The samples were harvested after 4-week and 8-week consolidation periods and analyzed histologically and by means of micro-computed tomography.ResultsPrimary, woven bone observed at the 4-week consolidation period was gradually replaced by lamellar bone at the 8-week consolidation period. Significant increase in bone volume was found in all groups (P < .001) and in bone mineral density in groups I (P−/M−; P < .001), III (P+/M−; P < .001), and IV (P+/M+; P = .013). Group III (P+/M−) showed significantly more new bone at the 8-week consolidation period compared with the other three groups (P = .001), with no differences observed in bone mineral density between groups at a given time-point.ConclusionsIn the present model, cortical bone perforations have more impact on the osteogenic process compared with the application of a collagen membrane.



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