Actinomyces israelii in Radicular Cysts: A Molecular Study

Publication date: Available online 21 February 2017
Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Author(s): Nathália Rodrigues Gomes, Marina Gonçalves Diniz, Thais dos Santos Fontes Pereira, Carlos Estrela, Luiz de Macedo Farias, Bruno Augusto Benevenuto de Andrade, Carolina Cavaliéri Gomes, Ricardo Santiago Gomez
ObjectivesTo investigate whether the microscopic filamentous aggregates observed in radicular cysts are associated with the molecular identification of Actinomyces israelli. Moreover, to verify whether this bacterium can be detected in radicular cyst specimens not presenting aggregates.Study DesignMicroscopic colonies suggestive of Actinomyces were found in eight out of 279 radicular cyst samples (case group). The case and control groups (n=12) (samples without filamentous colonies) were submitted to the semi-nested PCR to test the presence of A. israelii. DNA sequencing was performed to validate PCR results.ResultsTwo and three samples of the case and control groups, respectively, did not present a functional gDNA template and were excluded from the study. A. israelii was identified in all samples of the case group and in three out of nine samples of the control group.ConclusionAlthough A. israelli is more commonly identified in radicular cysts presenting filamentous aggregates, it also appears to be detected in radicular cysts without this microscopic finding.



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