Effect of Tempol on the prevention of irradiation-induced mucositisin miniature pigs

Abstract

Objective

The goals of this study were to (i) establish a useful miniature pig (minipig) model for irradiation-induced oral mucositis and (ii) evaluate the effect of Tempol to prevent its development.

Methods and Materials

Minipigs were irradiated with 6Gy for 5 consecutive days targeting the entire oral cavity. To prevent radiation damage minipigs were treated with 30 mg/kg Tempol 10 minutes before irradiation (n=4), while, the radiation-alone group was similarly injected with saline (n=4). Lesions were graded using an oral mucositis score and visual inspection every three days, and biopsy of multiple sites was performed at day 18. Weight, chest and abdomen circumference were measured every three days.

Results

Lesions began about 12 days post first irradiation fraction and healed about 30 days after irradiation. Epithelial thickness was calculated on the lingual and buccal mucosa on the 18^th day post first irradiation fraction. Tempol provided modest protection from ulceration after irradiation using this treatment strategy.

Conclusions

This study established a useful large animal model for irradiation-induced oral mucositis, and showed modest beneficial effects of Tempol in limiting tissue damage. The latter finding may be potentially valuable in preventing oral mucositis in patients receiving irradiation for head and neck cancers.

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