The proteomic profile of the acquired enamel pellicle according to its location in the dental arches

Publication date: Available online 3 March 2017
Source:Archives of Oral Biology
Author(s): Talita Mendes da Silva Ventura, Luiza de Paula Silva Cassiano, Cíntia Maria de Souza e Silva, Even Akemi Taira, Aline de Lima Leite, Daniela Rios, Marília Afonso Rabelo Buzalaf
ObjectiveThis study evaluated the variation in the protein profile of the acquired enamel pellicle (AEP) formed in vivo according to its location in the dental arches.DesignThe AEP was formed for 120minutes in 9 volunteers. Pellicle formed at upper+lower anterior facial (ULAFa; teeth 13-23 and 33-43), upper anterior palatal (UAPa; teeth 13-23), lower anterior lingual (LALi; teeth 33-43), upper+lower posterior facial (ULPFa; teeth 14-17 24 to 27, 34 to 37 and 44 to 47), upper posterior palatal (UPPa; teeth 14 to 17 and 24 to 27) and lower posterior lingual (LPLi; teeth 34 to 37 and 44 to 47) regions was collected separately and processed for analysis by label-free LC-ESI-MS/MS.ResultsThree-hundred sixty three proteins were identified in total, twenty-five being common to all the locations, such as Protein S100-A8, Lysozyme C, Lactoferrin, Statherin, Ig alpha-2, ALB protein, Myeloperoxidase and SMR3B. Many proteins were found exclusively in the AEP collected from one of the regions (46-UAPa, 33-LALi, 59-ULAFa, 31-ULPFa, 44-LPLi and 39-UPPa).ConclusionsThe protein composition of the AEP varied according to its location in the dental arches. These results provide important insights for understanding the differential protective roles of the AEP as a function of its location in the dental arches.



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