Impact of autologous bone marrow-derived stem cells on degenerative changes of articulating surfaces associated with arthritic TMJ; an experimental study in rabbits

Publication date: Available online 10 May 2017
Source:Journal of Oral and Maxillofacial Surgery
Author(s): Ahmed Abdelfatah ZaKi, Mohamed Zaghloul, Mohamed E. Helal, Noha Ahmed Mansour, Mohammed E. Grawish
PurposeThis study evaluated the effect of bone marrow-derived stem cells (BMSCs) on degenerative changes of TMJ associated with bovine collagen type II (BCII) induced-arthritis.Materials and MethodsFifty rabbits were divided into three groups as follows: group I (n=10); received no treatment. Group II (n=20); assigned into two subgroups according to the treatment type. A subgroup of 10 rabbits was injected intraarticular (IA) with phosphate buffered saline (PBS) while the other (n=10) was injected IA with combined therapy of PBS & BMSCs. Group III (n=20); handled as group II and instead before treatment with PBS or combined therapy of PBS & BMSCs, they were injected with oil emulsion of BCII. Rabbits were euthanized after the 3^rd week and the joints were processed for histology.ResultsCompared to TMJs of subgroup III treated with PBS, TMJs of subgroup III treated with combined therapy showed improvements in all parameters tested. Mean values for this subgroup regarding fibrous condylar covering, cartilaginous condylar plate, subchondral bone, articular eminence, upper joint cavity, lower joint cavity and disc thickness were 4.04±0.13, 5.09±0.08, 25.29±0.36, 10.54±0.36, 4.53±0.44, 3.21±0.27, and 24.52±0.41, respectively. Significant differences were found between subgroups mean values of group II and group IIII.Conclusions: BMSCs safely and effectively repair degenerative changes of rabbits' TMJ associated with BCII induced-arthritis.



http://ift.tt/2q1mcOE